Detection of Encephalitozoon spp. from Human Diarrheal Stool and Farm Soil Samples in Korea

다국어 초록 (Multilingual Abstract)

Microsporidia are eukaryotic organisms that cause zoonosis and are major opportunisticpathogens in HIV-positive patients. However, there is increasing evidence that theseorganisms can also cause gastrointestinal and ocular infections in immunocompetentindividuals. In Korea, there have been no reports on human infections with microsporidiato date. In the present study, we used real-time PCR and nucleotide sequencing to detectEncephalitozoon intestinalis infection in seven of 139 human diarrheal stool specimens(5%) and Encephalitozoon hellem in three of 34 farm soil samples (8.8%). Genotypeanalysis of the E. hellem isolates based on the internal transcribed spacer 1 and polar tubeprotein genes showed that all isolates were genotype 1B. To our knowledge, this is the firstreport on human E. intestinalis infection in Korea and the first report revealing farm soilsamples as a source of E. hellem infection. Because microsporidia are an important publichealth issue, further large-scale epidemiological studies are warranted.

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