Overexpression of the rice basic leucine zipper transcription factor OsbZIP12 confers drought tolerance to rice and makes seedlings hypersensitive to ABA

다국어 초록 (Multilingual Abstract)

Abiotic stresses such as drought, high salinity,and cold are key factors in determining crop yield. Rice is anotoriously drought-susceptible crop due in part to its smallroot system. Abscisic acid (ABA) involves in abiotic stressresistance in plants. The basic leucine zipper (bZIP) transcriptionfactors play important roles in the ABA signalingpathway in plants. OsbZIP12 is a member of the group EbZIP transcription factor family in rice (Oryza sativa).
Expression of OsbZIP12 is induced by abiotic stresses andABA as well as sugar in rice. It is rapidly and stronglyinduced by drought stress. Corresponding with expressionpatterns, several stress and sugar-associated cis-actingelements were found in the OsbZIP12 promoter region.
Transgenic rice overexpressing OsbZIP12 showed significantlyimproved drought tolerance and hypersensitivity toABA. The elevated levels of expression of the ABAresponding LEA3 and Rab16 genes in transgenic rice mayenhance drought tolerance. These results indicate thatOsbZIP12 is a positive regulator of ABA signaling anddrought stress tolerance in rice and that OsbZIP12 could beinvolved in crosstalk among stress, hormone, and sugarsignaling. We propose that OsbZIP12 is an importantplayer in rice for conferring ABA-dependent drought toleranceand has high potential for use in the geneticimprovement of stress tolerance.

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