Larvicidal activity of acetone extract and green synthesized silver nanoparticles from Allium sativum L. (Amaryllidaceae) against the dengue vector Aedes aegypti L. (Diptera: Culicidae)

다국어 초록 (Multilingual Abstract)

Mosquito vectors of major human diseases are currently controlled using chemical and biological products.
Extensive insecticide use has led to resistance development and human/environmental health risks, and alter native sustainable control options are needed; in this study, activity of an extract of garlic (Allium sativum; Amaryllidaceae), and silver nanoparticles (AgNPs) synthesized from the extract, were evaluated against 2nd and 3rd instar larvae of the yellow fever mosquito, Ae. aegypti (Diptera: Culicidae). Synthesis of AgNPs was confirmed using UV–Vis spectroscopy, and characterised using powdered X-ray diffraction, Fourier transform infrared spectroscopy, and scanning electron microscopy. Larvae were exposed to five concentrations (50, 100, 150, 200, 250 ppm) of garlic extract or synthesized AgNPs, with distilled water and silver nitrate solution (1 mM) as controls. The mortality of larvae was recorded after 6, 12, 24, 36, and 48 h following addition of the respective extracts.
Dose- and time-dependent toxicity were recorded in both treatment groups with no mortality in control groups. Exposure to AgNPs at 250 ppm for 48 h yielded 100% mortality for both larval instars, with corre sponding LC 50 values of 44.77 (2nd) and 62.82 ppm (3rd). Exposure to garlic extract resulted in similar 48-hour mortality (99 ± 0.77% (2nd) and 98 ± 1.10% (3rd), but consistently higher LC 50 values after all exposure times compared to AgNPs (e.g. 48-hour exposure: 108.42 ppm (2nd), 129.11 ppm (3rd), suggesting that AgNPs may potentially be used at lower concentrations for Ae. aegypti control.

참고문헌 (Reference)

1 Who, "World Health Organization: Guidelines for laboratory and field testing of mosquito larvicides"

2 Tunçsoy, B.S., "Toxicity of nanoparticles: A review" 1 (1): 2018

3 Santoro, C., "Threedimensional graphene nanosheets as cathode catalysts in standard and supercapacitive microbial fuel cell" 356 : 371-380, 2017

4 Mikaili, P., "Therapeutic uses and pharmacological properties of garlic, shallot, and their biologically active compounds, Iran" 16 : 1031-1048, 2013

5 Bhatt, S., "The global distribution and burden of dengue" 496 (496): 504-507, 2013

6 Vogel, A. I., "Textbook of practical organic chemistry, including qualitative organic analysis" The English Language Book Society and Longman 1368-, 1978

7 Ga’al, H., "Synthesis, characterization and efficacy of silver nanoparticles against Aedes albopictus larvae and pupae" 144 : 49-56, 2018

8 Bouqellah, N. A., "Synthesis of eco-friendly silver nanoparticles using Allium sp. And their antimicrobial potential on selected vaginal bacteria" 26 (26): 1789-1794, 2019

9 Singh, A., "Studies on larvicidal activity of some plant extracts against Filarial vector Culex quinquefasciatus" 6 : 1-6, 2016

10 Malabadi, R. B., "Silver nanoparticles synthesized by in-vitro derived plants and callus cultures of Clitoria ternatea; evaluation of antimicrobial activity" 3 : 26-38, 2012

11 Mao, B. H., "Silver nanoparticles have lethal and sublethal adverse effects on development and longevity by inducing ROS-mediated stress responses" 8 (8): 2445-, 2018

12 Waseem Akram ; Faisal HAFEEZ ; Unsar Naeem ULLAH ; 김연국 ; Aftab HUSSAIN ; 이종진, "Seasonal distribution and species composition of daytime biting mosquitoes" 한국곤충학회 39 (39): 107-113, 2009

13 Brady, O. J., "Refining the global spatial limits of dengue virus transmission by evidence-based consensus" 6 (6): e1760-, 2012

14 Shankar, S. S., "Rapid synthesis of Au, Ag, and bimetallic Au core-Ag shell nanoparticles using Neem(Azadirachta indica)leaf broth" 275 (275): 496-502, 2004

15 Jinu, U., "Potential larvicidal activity of silver nanohybrids synthesized using leaf extracts of Cleistanthus collinus (Roxb.) Benth. ex Hook.f. and Strychnosnux-vomica L. nux-vomica against dengue, Chikungunya and Zika vectors" 101 : 163-171, 2017

16 Benelli, G., "Plant-mediated biosynthesis of nanoparticles as an emerging tool against mosquitoes of medical and veterinary importance : a review" 115 (115): 23-34, 2016

17 Satyavani, K., "Plant mediated synthesis of biomedical silver nanoparticles by leaf extract of Citrullus colosynthis" 1 : 95-101, 2011

18 Raut, R. W., "Phyto synthesis of silver nanoparticles using Gliricidia sepium(Jaeq)" 5 : 117-122, 2009

19 Rauf, M., "Outbreak of chikungunya in Pakistan" 17 : 258-, 2017

20 Ferreira-de-Lima, V. H., "Natural vertical transmission of dengue virus in Aedes aegypti andAedes albopictus: a systematic review" 11 : 77-, 2018

21 Athanassiou, C. G., "Nanoparticles for pest control : current status and future perspectives" 91 : 1-15, 2018

22 Benelli, G., "Nanoparticles for mosquito control : challenges and constraints" 29 (29): 424-435, 2017

23 Shahzad, K., "Nanoformulations and their mode of action in insects : a review of biological interactions" 44 : 1-11, 2019

24 Schneider, C. A., "NIH Image to ImageJ : 25 years of image analysis"" 9 (9): 671-675, 2012

25 Becker, N., "Mosquitoes and Their Control" Springer Berlin Heidelberg 2010

26 Mathivanan, T., "Mosquito larvicidal and phytochemical properties of Ervantania coronaria(Family Apocynaceae)" 47 : 178-180, 2000

27 Subramaniam, J., "Mosquito larvicidal activity of Aloe vera (Family: Liliaceae) leaf extract and Bacillus sphaericus, against Chikungunya vector. Aedes aegypti" 19 (19): 503-509, 2012

28 Qasim, M., "Mosquito (Diptera: Culicidae) of Murree Hills. Punjab. Pakistan" 46 : 523-529, 2014

29 Bar, A., "Morphology and Morphometry of Aedes aegypti Larvae" 3 : 1-21, 2013

30 Nair, P. M. G., "Modulation in the mRNA expression of ecdysone receptor gene in aquatic midge, Chironomus riparius upon exposure to nonylphenol and silver nanoparticles" 33 (33): 98-106, 2012

31 Yasur, J., "Lepidopteran insect susceptibility to silver nanoparticles and measurement of changes in their growth, development and physiology" 124 : 92-102, 2015

32 Arivoli, S., "Larvicidal activity of botanicals against the filarial vector Culex quinquefasciatus Say(Diptera : Culicidae)" 2 : 13-17, 2011

33 Jawale, C., "Larvicidal activity of Cestrum nocturnum on Aedes aegypti" 5 : 39-40, 2010

34 Tennyson, S., "Larvicidal activity of Ageratum houstonianum Mill. (Asteraceae) leaf extracts against Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae)." 5 : S73-S76, 2015

35 Kalu, I. G., "Larvicidal activities of ethanol extract of Allium sativum(garlic bulb)against the filarial vector, Culex quinquefasciatus" 4 : 496-498, 2010

36 Collins, T. J., "ImageJ for microscopy" 43 (43): 25-30, 2007

37 Nair, P. M. G., "Identification, characterization and expression profiles of Chironomus riparius glutathione S-transferase(GST)genes in response to cadmium and silver nanoparticles exposure" 101 (101): 550-560, 2011

38 Pavela, R., "History presence and perspective of using plant extracts as commercial botanical insecticides and farm products for protection against insects - a review" 52 (52): 229-241, 2016

39 Priya, S., "Green synthesis of silver nanoparticles using Calothropis gigantea and their potential mosquito larvicidal property" 2 : 128-137, 2014

40 Saha, M., "Green biosynthesis of silver nanoparticle using garlic, Allium sativum with reference to its antimicrobial activity against the pathogenic strain of Bacillus sp. And Pseudomonas sp. infecting goldfish. Carassius auratus" 72 (72): 180-186, 2019

41 Regoli, F., "Glutathione, glutathione–dependent and antioxidant enzymes in mussel, Mytilus galloprovincialis, exposed to metals under field and laboratory conditions : implications for the use of biochemical biomarkers" 31 (31): 143-164, 1995

42 Jones, K. E., "Global trends in emerging infectious diseases" 451 (451): 990-993, 2008

43 Gomes, T., "Genotoxicity of copper oxide and silver nanoparticles in the mussel Mytilus galloprovincialis" 84 : 51-59, 2012

44 Ahamed, M., "Genotoxic potential of copper oxide nanoparticles in human lung epithelial cells" 396 (396): 578-583, 2010

45 Vijayakumar, S., "Garlic clove extract assisted silver nanoparticle –Antibacterial, antibiofilm, antihelminthic, anti-inflammatory, anticancer and ecotoxicity assessment" 198 : 2019-, 2019

46 Iqbal, J., "Evaluation of larvicidal efficacy of indigenous plant extracts against Culex quinquefasciatus(Say)under laboratory conditions" 42 : 207-215, 2018

47 Waris, M., "Evaluation of larvicidal efficacy of Ricinus communis(Castor)and synthesized green silver nanoparticles against Aedes aegypti L" 27 (27): 2403-2409, 2020

48 Habeeb, S. M., "Efficiency of Allium cepa and Commiphora molmol as a Larvicidal agent against fourth stage larvae of C. pipiens (Diptera: Culicidae)" 5 : 196-203, 2009

49 Rahuman, A. A., "Efficacy of larvicidal botanical extracts against Culex quinquefasciatus Say(Diptera : Culicidae)" 104 (104): 1365-1372, 2009

50 Gagn´e, F., "Ecotoxicity of CdTe quantum dots to freshwater mussels : Impacts on immune system, oxidative stress and genotoxicity" 86 (86): 333-340, 2008

51 Subramaniam, J., "Eco-friendly control of malaria and arbovirus vectors using the mosquito fish Gambusia affinis and ultra-low dosages of Mimuso pselengi synthesized silver nanoparticles : towards an integrative approach" 22 (22): 20067-20083, 2015

52 WHO, "Dengue and severe dengue"

53 Pattnaik, A., "Current Status of Zika Virus Vaccines : Successes and Challenges" 8 : 266-285, 2020

54 Fouad, H., "Controlling Aedes albopictus and Culex pipiens pallens using silver nanoparticles synthesized from aqueous extract of Cassia fistula fruit pulp and its mode of action" 46 (46): 558-567, 2018

55 Kalimuthu, K., "Control of dengue and Zika virus vector Aedes aegypti using the predatory copepod Megacyclops formosanus : synergy with Hedychium coronarium-synthesized silver nanoparticles and related histological changes in targeted mosquitoes" 109 : 82-96, 2017

56 Tiwary, M., "Chemical composition and larvicidal activities of the essential oil of Zanthoxylum armatum DC(Rutaceae)against three mosquito vectors" 44 : 198-204, 2007

57 Rai, M., "Broad-spectrum bioactivities of silver nanoparticles : the emerging trends and future prospects" 98 (98): 1951-1961, 2014

58 Agalya Priyadarshini, K., "Biolarvicidal and pupicidal potential of silver nanoparticles synthesized using Euphorbia hirta against Anopheles stephensi Liston(Diptera : Culicidae)Parasitol" 111 (111): 997-1006, 2012

59 Vivek, M., "Biogenic silver nanoparticles by Gelidiella acerosa extract and their antifungal effects" 3 : 143-148, 2011

60 Parthiban, E., "Biocompatible green synthesized silver nanoparticles impact on insecticides resistant developing enzymes of dengue transmitted mosquito vector" 1 : 2019

61 Ahmed, S., "A review on plant extract mediated synthesis of silver nanoparticles for antimicrobial applications : a green expertise" 7 : 17-28, 2016

62 Shaalan, E. -S., "A review of botanical phytochemicals with mosquitocidal potential" 31 (31): 1149-1166, 2005

63 Surendran, S. N., "A note on the larvicidal efficacy of saponin constituted crude extracts of plant and animal origin against Aedes aegypti" 37 : 215-217, 2009

64 Sardar, T., "A mathematical model of dengue transmission with memory" 22 (22): 511-525, 2015