산란계에 편백나무 정유 살포가 닭진드기에 미치는 영향 = Effect of Spraying Chamaecyparis obtusa Essential Oil on the Elimination of Red Mite (Dermanyssus gallinae) in Laying Hens

국문 초록 (Abstract)

본 연구는 편백나무 정유를 70% 함유한 FC-S 살포액을 배율별로 희석하여 살포시에 닭진드기 제거와 안전성에 미치는 영향을 구명하고자 실행하였다. 닭진드기에 감염된 산란계 300수를 5개 처리구와 5반복 반복당 12수씩 18일간 배치하였다. 각 처리구의 살포액은 FC-S를 0배(원액, FC-S 0), 50배(FC-S 50) 및 100배(FC-S 100)로 희석한 시료와 증류수(NC) 및 시중에서 판매되는 천연살충제(PC)를 이용하였다. 닭진드기 숫자, 산란율, 난중은 매일 확인하였고, 계란품질과 혈액성상은 사양실험 종료 후 측정하였다. 안전성 검사를 위하여 산란계의 사료섭취, 호흡기증상, 침울, 설사 및 폐사 증상을 확인하였다. 본 연구결과, FC-S 0을 살포시에 80% 이상의 닭진드기가 제거되었으며(P<0.01), FC-S 50 처리구도 PC 처리구보다 개선되었다. FC-S 100 처리구는 PC 처리구보다 진드기 제거에 미치는 영향이 적었지만, NC 처리구에 비하여 닭진드기 제거는 매우 개선되었다(P<0.01). FC-S를 살포한 후 산란계의 생산성, 계란품질, 혈액성상 및 안전성에 미치는 영향은 처리구간에 유사하였으며, 통계적 차이가 없었다. 본 연구결과, FC-S의 살포는 산란계에 미치는 영향은 없었으므로, 닭진드기에만 선택적으로 작용하였다고 사료되며, FC-S 0∼50배 희석배율의 살포는 상용화되어 판매되는 살포액보다 효과적으로 닭진드기를 제거하였다.

다국어 초록 (Multilingual Abstract)

The purpose of this study was to evaluate the influence and safety of spraying Farm Clean-S (FC-S) containing 70% Chamaecyparis obtusa essential oil on the elimination of red mite in laying hens. In total, 300 laying hens (ISA brown) aged 72 weeks and infected with red mite were assigned to individual cages for five treatments with five replicates for 18 days. Treatments consisted of distilled water (negative control; NC), commercial spray product (positive control; PC), FC-S 0 (original concentration), FC-S 50 (50 times diluted) and FC-S 100 (100 times diluted). The number of red mites, egg production and weight of each group were evaluated daily throughout the whole experimental period. Egg quality and blood biochemical parameters were measured at the end of the experiment. Safety tests were confirmed by feed intake, respiration, lethargy, diarrhea and mortality symptoms after the feeding trial. As the result, more than 80% of red mites were eliminated by the FC-S 0 spray treatment, and FC-S 50 also showed a higher elimination percentage than did PC (P<0.01). However, the elimination effect of the FC-S 100 spray was lower than that of the PC, but higher than that of the NC (P<0.01). These results confirmed that the optimum FC-S dilution rate ranged from 0∼50 times with distilled water to eliminate the red mite effectively. The performance, egg quality, blood parameter and safety tests were not significantly different among treatments. Thus, FC-S 0 and FC-S 50 spray treatments can effectively and sustainably control red mite infestations without detrimental effects on the performance, egg quality and plasma biochemical properties of laying hens.

목차 (Table of Contents)

• ABSTRACT
• 서론
• 재료 및 방법
• 결과
• 고찰
• 적요
• REFERENCES

참고문헌 (Reference)

1 남기연, "향나무 심재 추출물로 염색된 직물의 방미성과 집먼지 진드기 기피효과" 한국생활과학회 22 (22): 687-699, 2013

2 김해성, "편백나무 정유의 소취효과 및 항균력 평가" 한국냄새환경학회 8 (8): 101-107, 2009

3 George DR, "Toxicity of plant essential oils to different life stages of the poultry red mite, Dermanyssus gallinae, and non target invertebrates" 24 (24): 9-15, 2010

4 SAS, "SAS/STAT Software for PC"

5 George DR, "Repellence of plant essential oils to Dermanyssus gallinae and toxicity to the non-target invertebrate Tenebrio molitor" 162 (162): 129-134, 2009

6 Flochlay AS, "Poultry red mite (Dermanyssus gallinae) infestation: A broad impact parasitological disease that still remains a significant challenge for the egg-laying industry in Europe" 10 (10): 357-362, 2017

7 Dalton JP, "Parasite vaccines-A reality?" 98 (98): 149-167, 2001

8 Duncan DB, "Multiple range and multiple F tests" 11 (11): 1-42, 1955

9 George DR, "Mode of action and variability in efficacy of plant essential oils showing toxicity against the poultry red mite, Dermanyssus gallinae" 161 (161): 276-282, 2009

10 Kilpinen O, "Influence of Dermanyssus gallinae and Ascaridia galli infections on behaviour and health of laying hens (Gallus gallus domesticus)" 46 (46): 26-34, 2005

11 Kim EH, "Extraction, finishing technology, and status of phytoncide" 5 (5): 71-82, 2010

12 Yamauchi K, "Exterminating effect of wood vinegar to red mites and its safety to chickens" 51 (51): 327-332, 2014

13 Abdelfattah EM, "Essential oils: Effects of application rate and modality on potential for combating northern fowl mite infestations" 32 (32): 1-7, 2018

14 Camarda A, "Efficacy of a novel neem oil formulation ($RP03^{TM}$) to control the poultry red mite Dermanyssus gallinae" 32 (32): 1-8, 2018

15 Ding X, "Effects of essential oils on performance, egg quality, nutrient digestibility and yolk fatty acid profile in laying hens" 3 (3): 127-131, 2017

16 Hiramatsu Y, "Effect of volatile matter from wood chips on the activity of house dust mites and on the sensory evaluation of humans" 47 (47): 13-17, 2001

17 Mori T, "Effect of softwood thin veneers in tatami on the activity of the house dust mite Dermatophagoides pteronyssinus" 48 (48): 163-164, 2002

18 Kim SI, "Contact and fumigant toxicity of oriental medicinal plant extracts against Dermanyssus gallinae (Acari: Dermanyssidae)" 145 (145): 377-382, 2007

19 Valiente Moro C, "Colonization and organ invasion in chicks experimentally infected with Dermanyssus gallinae contaminated by Salmonella Enteritidis" 36 (36): 307-311, 2007

20 Mullens BA, "Beak condition and cage density determine abundance and spatial distribution of northern fowl mites, Ornithonyssus sylviarum, and chicken body lice, Menacanthus stramineus, on caged laying hens" 89 (89): 2565-2572, 2010

21 Kim SI, "Acaricidal activity of plant essential oils against Dermanyssus gallinae (Acari: Dermanyssidae)" 120 (120): 297-304, 2004

22 Jang YS, "Acaricidal activity of active constituent isolated in Chamaecyparis obtusa leaves against Dermatophagoides spp." 53 (53): 1934-1937, 2005

23 Di Palma A, "A gallery of the key characters to ease identification of Dermanyssus gallinae (acari: gamasida: dermanyssidae) and allow differentiation from Ornithonyssus sylviarum (acari: gamasida: macronyssidae)" 5 (5): 104-113, 2012