Cycloheximide가 膵臟腺房細胞에 미치는 影響에 關한 微細形態學的 硏究 = Effects of Cycloheximide on the Fine Structures of Pancreatic Acinar Cells

국문 초록 (Abstract)

蛋白合成障碍가 膵臟腺房細胞의 微細構造 및 自家貪食力에 미치는 影響을 알아보기 爲해 흰쥐에 cycloheximide와 自家貪食亢進劑인 neutral red를 單獨 또 倂合 投與하여 惹起된 微細形態學的 變化를 電子顯微鏡으로 觀察하였던 바를 要約하면 다음과 같다.
Cycloheximide는 膵臟腺房細胞에 比較的 初期에서부터 Golgi complex의 腫脹, ER膜에 附着된 ribosome의 脫落을 主로 한 輕한 變化를 일으켰다. 60分이 經過하면 ER 및 mitochondria의 輕한 腫脹, 脫落된 ribosome의 破壞 및 消失等을 볼 수 있었으나 그 程度는 輕微하였다.
Autophagic vacuole의 計數는 對照群이 한 腺房當 10.1±2.2個, neutral red投與群이 54.4±4.9個, cycloheximide와 neutral red를 同時에 投與한 群에서는 21.5±4.1개, cycloheximide投與 30分 後 neutral red 投與群에서는 9.7±2.7個이었다. 이와 같은 事實은 cycloheximide가 autophagic vacuole形成에 抑制的 效果가 있다는 것을 意味하며 autophagic vacuole의 形成에는 어떤 形態로든 새로운 蛋白合成이 必要하다는 것을 示唆하는 것이라 생각된다.

다국어 초록 (Multilingual Abstract)

This study was carried out to investigate the effects of cycloheximide, known as an inhibitor of protein synthesis at the level of polypetide elongation, on the fine structures of pancreatic acinar cells. Also studied was the effects of protein synthesis inhibition by cycloheximide on the neutral red-induced autophagocytosis.
Twenty-one male albino rats, maintained on water only for 12 hours, weighing 200㎎. in average, were divided into 5 groups, 3 rats for each groups except Group 2.
Group 1: This is to serve as a control group. Two consecutive doses of 0.4㎖. of saline per 100 gm. of body weight were given intraperitoneally to 3 rats at interval of 30 minutes.
Group 2: A dose of 2 mg. of cycloheximide per 100gm. of body weight was given to 9 rats intraperitoneally and 3 animals each were sacrificed 30, 60 and 90 minutes after injection, respectively.
Group 3: A dose of 2mg. of neutral red per 100gm. of body weight was given to 3 rats intraperitoneally, 30 minutes after intraperitoneal injection of 0.4 ㎖ of saline per gm. of body weight.
Group 4: A dose of 2㎎. of neutral red per 100gm. of body weight was given to 3 rats intraperitoneally, 30 minutes after intraperitioneal injection of 2㎎. of cycloheximide per 100gm. of body weight.
Group 5: Simultaneous intraperitoneal injection of 2 ㎎. of neutral red and 2㎎. of cycloheximide per 100gm. of body weight was given to 3 rats.
All animals were sacrificed 60 minutes after the the last injection except Group 2. Pancreas extirpated for both light and electron microscopic studies.
The results were summarized as follow:
Cycloheximide induced moderate dilatation of Golgi complex aad detachment of ribosomes from RER membrane from early stage (sacrificed 30 minute after injection). Mild swelling of RER and mitochondria along with destruction and disappearance of detached ribosomes were noted in the animals sacrificed 60 and 90 minutes after injection (Group 2).
The number of autophagic vacules per acinus was 10.1±2.2 in the control group (Group 1); 54.4±4.9 in neutral red only group (Group 3); 21.5±4.1 in neutral red simultaneous with cycloheximide group(Group 5); 9.7±2.7 in the group with neutural red administered 30 minutes after cycloheximide injection(Group 4). The results showed marked inhibitory effects of cycloheximide on the neutral red-induced autophagocytosis, suggesting need for do novo synthesis of protein in the formation of the autophagic vacuoles.