Ginsenoside-Rp1-induced apolipoprotein A-1 expression in the LoVo human colon cancer cell line

다국어 초록 (Multilingual Abstract)

Background: Ginsenoside Rp1 (G-Rp1) is a novel ginsenoside derived from ginsenoside Rk1. This compoundwas reported to have anticancer, anti-platelet, and anti-inflammatory activities. In this study, weexamined the molecular target of the antiproliferative and proapoptotic activities of G-Rp1.
Methods: To examine the effects of G-Rp1, cell proliferation assays, propidium iodine staining, proteomicanalysis by two-dimensional gel electrophoresis, immunoblotting analysis, and a knockdown strategywere used.
Results: G-Rp1 dose-dependently suppressed the proliferation of colorectal cancer LoVo cells andincreased their apoptosis. G-Rp1 markedly upregulated the protein level of apolipoprotein (Apo)-A1 inLoVo, SNU-407, DLD-1, SNU-638, AGS, KPL-4, and SK-BR-3 cells. The knockdown of Apo-A1 by its smallinterferingRNA increased the levels of cleaved poly(ADP-ribose) polymerase and p53 and diminished theproliferation of LoVo cells.
Conclusion: These results suggest that G-Rp1 may act as an anticancer agent by strongly inhibiting cellproliferation and enhancing apoptosis through upregulation of Apo-A1.

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