Preparation of Bacteria Microarray Using Selective Patterning of Polyelectrolyte Multilayer and Poly(ethylene glycol)-Poly(lactide) Diblock Copolymer

다국어 초록 (Multilingual Abstract)

This study reports a simple but efficient method for create bacteria microarrays on a predesigned functional surface consisting of two distinctive regions; a bacterial immobilizing area and a nonimmobilizing region. The functionalized surface was fabricated by a combination of self-assembled polyelectrolyte multilayers (PEL) and micromolding in the capillaries (MIMIC) of poly(ethylene glycol)-poly(D,L-lactide) diblock copolymer (PEGPLA).
The PEL region provides bacterial immobilization, and the nonspecific binding of bacteria was prevented using PEG-PLA as passivation molecules. The topological change in the functionalized surface was characterized by atomic force microscopy (AFM), which suggested the occurrence of a laterally homogeneous and well defined surface modification. An analysis of the fluorescence signals from the bacteria microarray indicates that the bacteria are viable and grow after immobilization on patterned surface features. The rapid fabrication of two different functionalities on the surface results in a uniform bacteria pattern with a high signal to noise ratio and shape flexibility,such as lines, squares, and circles. Moreover, the pattern size could be controlled from 20 to 100 μm.

참고문헌 (Reference)

1 I. Barbulovic-Nad, 26 : 237-, 2006

2 X. C. Zhou, 6 : 1415-, 2006

3 H. Zhu, 5 : 40-, 2001

4 W. J. Lee, 129 : 841-, 2008

5 C. S. Lee, 22 : 891-, 2007

6 D. S. Ginger, 43 : 30-, 2004

7 J. F. Millau, 8 : 1713-, 2008

8 T. G. Fernandes, 80 : 6633-, 2008

9 N. Bahlis, 110 : 138-, 2007

10 S. Rozhok, 22 : 11251-, 2006

11 N. R. Thirumalapura, 309 : 48-, 2006

12 L. P. Xu, 7 : 2068-, 2007

13 H. W. Shim, 4 : 325-, 2006

14 G. Moskovits, 88 : 1815-, 1964

15 D. B. Weibel, 21 : 6436-, 2005

16 J. Cho, 13 : 1076-, 2001

17 J. Cho, 22 : 1356-, 2006

18 J. H. Lee, 64 : 126-, 2008

19 D. L. Elbert, 5 : 177-, 1998

20 S. Krishnan, 18 : 3405-, 2008

21 H. W. Shim, 22 : 3188-, 2007

22 H. W. Shim, 9 : 1204-, 2009

23 D. T. Haynie, 6 : 2895-, 2005

24 K. Bucior, 126 : 9-, 2007

25 S. Rauf, 1721-, 2006

26 E. J. Calvo, 116 : 47-, 2000

27 H. Abbasnezhad, 62 : 36-, 2008

28 A. M. Gallardo-Moreno, 17 : 1877-, 2003

29 김성규, "Nanopatterning of Proteins Using Composite Nanomold and Self-Assembled Polyelectrolyte Multilayers" 한국고분자학회 17 (17): 232-239, 2009