Synergistic effect of peroxiredoxin II antisense on cisplatininduced cell death

다국어 초록 (Multilingual Abstract)

Peroxiredoxin II (Prx II) is known not only to protectcells from oxidative damage caused by hydrogen per-oxide (H2O2), but also to endow cancer cells with resis-tance to both H 2O2 and cisplatin and to grant themradioresistance. In this study, we examined whetherPrx II antisense could enhance cisplatin-induced celldeath. When gastric cancer cells were transfected withvarious concentrations of Prx II antisense plasmid,pPrxII/AS, and then treated with the same concentra-tions of cisplatin, Prx II antisense enhanced cisplatin-induced cell death. The combination index (CI) at alldoses of the combination was below 1, indicating thatPrx II antisense sensitized cisplatin-induced cell death.This synergism was also observed in the cells trans-fected with a Prx II antisense oligomer. Our presentresults, therefore, suggest that Prx II antisense wouldbe a very good sensitizer for cisplatin, and that Prx II asa target for chemosensitizers constitutes a promisingavenue for future research.