Aspergillus niger를 이용한 당 합성 폐수 처리와 산업 배지에서 Bacillus licheniformis의 세포 성장에 따른 α-amylase 생성에 관한 연구 = Studies on the Glucose Synthetic Wastewater Treatment by Aspergillus niger and Production of α-amylase on Cell Growth by Bacillus licheniformis in Industrial Medium

다국어 초록 (Multilingual Abstract)

The present studies were performed to investigate the glucose synthetic wastewater treatment by Aspergillus niger and production of α -amlyase on cell growth by Bacillus licheniformis in industrial medium. The optimal temperature and pH on the cell growth by Aspergillus niger were 25℃ and 6.0, respectively. The exponential growth during the incubation time of Aspergillus niger was up to 120 hrs and the cell productivity was 150 ㎎/L. According to increase the glucose concentration, the cell growth was gradually increased up to 3 g/L, but it was found that the cell growth was decreased with respect to increase the glucose concentration at more than 3 g/L. In the case of the glucose synthetic wastewater, the 95% of COD and 96% of glucose were removed at 900 ㎎/L in average concentration of COD from influent wastewater at HRT for 24 hrs.
A study on production of α-amlyase on cell growth by Bacillus lichenifomis expressed in E. coli at the industrial medium was obtained the following results. Cell growth increased according to increase of initial concentration of carbon sources containing rice, potato and sweet potato powder. It was showed that the maximum productivity for cell growth was 6.16 g/L at the rice powder of 30 g/L, and the maximum production of α -amylase on cell growth was 347.20 unit/㎖ at the potato powder of 30 g/L. By-product production for cell growth were as following, lactic acid > acetic acid > ethanol > propionic acid > methanol

목차 (Table of Contents)

• 목차 = ⅰ
• LIST OF TABLES = ⅲ
• LIST OF FIGURES = ⅳ
• ABSTRACT = ⅵ
• 제1장. Aspergillus niger를 이용한 당 합성 폐수 처리 = 1
• 1.1. 서론 = 2
• 1.2. 재료 및 방법 = 4
• 1.2.1. 실험 균주 = 4
• 1.2.2. 사용 배지 = 4
• 1.2.3. Glucose 합성 배지 = 4
• 1.2.4. Glucose 정량 방법 = 4
• 1.2.4.1. Alkaline CuSO_(4) 용액 = 4
• 1.2.4.2. Arsenomolybdate 용액 = 7
• 1.2.4.3. 실험 방법 = 7
• 1.2.5. COD 분석 방법 = 7
• 1.2.5.1. 사용 시약 = 7
• 1.2.5.2. 실험 방법 = 9
• 1.2.6. 건조 균체량 측정 방법 = 9
• 1.2.7. 실험 장치 및 반응 방법 = 9
• 1.3. 결과 및 고찰 = 12
• 1.3.1. 세포 성장에 있어 배양 온도의 영향 = 12
• 1.3.2. 세포 성장에 있어 pH의 영향 = 14
• 1.3.3. 세포 성장에 있어 성장 곡선 = 16
• 1.3.4. Glucose 농도에 따른 세포 성장 = 20
• 1.3.5. Aspergillus를 이용한 Glucose 합성 모델 폐수 처리 = 22
• 1.4. 결론 = 27
• 참고문헌 = 29
• 제2장. 산업 배지에서 Bacillus licheniformis의 세포 성장과 α-amylase 생성에 관한 연구 = 31
• 2.1. 서론 = 32
• 2.1.1. 연구 배경 = 32
• 2.1.2. Amylase의 종류와 특성 = 35
• 2.2. 재료 및 실험 방법 = 38
• 2.2.1. 시약 및 기기 = 38
• 2.2.1.1. 시약 = 38
• 2.2.1.2. 사용 기기 = 38
• 2.2.2. 재료 = 38
• 2.2.2.1. 사용 균주 = 38
• 2.2.2.2. 배지 = 39
• 2.2.3. 세포 배양 = 41
• 2.2.4. HPLC 측정용 표준 시약 및 측정 방법 = 41
• 2.2.5. 회분식 배양 = 43
• 2.2.6. 세포 밀도 측정 = 43
• 2.2.7. α-amylase 활성 측정 = 46
• 2.3. 결과 및 고찰 = 47
• 2.3.1. 쌀가루에서의 세포 성장과 α-amylase 생성 = 47
• 2.3.2. 감자가루에서의 세포 성장과 α-amylase 생성 = 52
• 2.3.3. 고구마가루에서의 세포 성장과 α-amylase 생성 = 57
• 2.4. 결론 = 62
• 참고문헌 = 63