To study the signaling effect of insulin-like growth factor- I(IGF-1), transgenic mice containing IGF-1 Receptor(IGF-1R) cDNA fused to metallothionein promoter were produced by DNA microinjection into the pronucleus of mouse zygote. Three founders wer...
To study the signaling effect of insulin-like growth factor- I(IGF-1), transgenic mice containing IGF-1 Receptor(IGF-1R) cDNA fused to metallothionein promoter were produced by DNA microinjection into the pronucleus of mouse zygote. Three founders were produced with transgenic mice containing IGF-1R gene. Transgenic mice lines contained approximately 4∼20 copies of transgenes per cell and transmission of this gene into the progeny with Mendelian manner were determined. The founder mice were mated with normal mice to produce F₁mice and then F₂mice. Transmission rates of IGF-1R transgene in the progeny mice were 25∼60% in F₁generation and 40∼50% in F₂generation. The mRNA expression of IGF-1R transgene in liver was analyzed using RT-PCR for IGF-1R gene in liver. When body weights of transgenic pups were measured during 4, 10 and 14 weeks after birth, IGF-1R transgenic mice grew faster than non transgenic littermates. This study indicated that growth regulation by IGF-1 signaling through IGF-1R can be elucidated using IGF-1R transgenic mice.