Cr(VI) removal from aqueous solution by thermophilic denitrifying bacterium Chelatococcus daeguensis TAD1 in the presence of single and multiple heavy metals

다국어 초록 (Multilingual Abstract)

Cr(VI) pollution is increasing continuously as a result of ongoing industrialization. In this study, we investigated the thermophilic denitrifying bacterium Chelatococcus daeguensis TAD1, isolated from the biofilm of a biotrickling filter used in nitrogen oxides (NOX) removal, with respect to its ability to remove Cr(VI) from an aqueous solution. TAD1 was capable of reducing Cr(VI) from an initial concentration of 10 mg/L to non-detectable levels over a pH range of 7–9 and at a temperature range of 30–50°C. TAD1 simultaneously removed both Cr(VI) and NO3 −-N at 50°C, when the pH was 7 and the initial Cr(VI) concentration was 15 mg/L.
The reduction of Cr(VI) to Cr(III) correlated with the growth metabolic activity of TAD1. The presence of other heavy metals (Cu, Zn, and Ni) inhibited the ability of TAD1 to remove Cr(VI). The metals each individually inhibited Cr(VI) removal, and the extent of inhibition increased in a cooperative manner in the presence of a combination of the metals.
The addition of biodegradable cellulose acetate microspheres (an adsorption material) weakened the toxicity of the heavy metals; in their presence, the Cr(VI) removal efficiency returned to a high level. The feasibility and applicability of simultaneous nitrate removal and Cr(VI) reduction by strain TAD1 is promising, and may be an effective biological method for the clean-up of wastewater.

참고문헌 (Reference)

1 APHA, "standard methods for the examination of water and waste water"

2 Ghalib, A. K., "and metal detoxification potential of moderately thermophilic Bacillus cereus from geothermal springs of Himalaya" 57 : 554-560, 2014

3 Ibrahim, M.H.A., "Zobellella denitrificans strain MW1, a newly isolated bacterium suitable for poly(3-hydroxybutyrate)production from glycerol" 108 : 214-225, 2010

4 Arthanareeswaran, G., "Transport of copper, nickel and zinc ions across ultrafiltration membrane based on modified polysulfone and cellulose acetate" 7 : 131-139, 2012

5 Fan, J., "The synergism of temperature, pH and growth phases on heavy metal biosorption by two environmental isolates" 279 : 236-243, 2014

6 Chu, K.W., "Synergistic toxicity of multiple heavy metals is revealed by a biological assay using a nematode and its transgenic derivative" 61 : 53-64, 2002

7 Zhao, X. X., "Study on the production of NiR by Bacillus cereus Cr2 and the correlation between the enzyme and removal rate of Cr(VI)" 4 : 75-78, 2013

8 Saha, R., "Sources and toxicity of hexavalent chromium" 64 : 1782-1806, 2011

9 Sultan, S., "Reduction of toxic hexavalent chromium by Ochrobactrum intermedium strain SDCr-5 stimulated by heavy metals" 98 : 340-344, 2007

10 Slobodkina, G. B., "Reduction of chromate, selenite, tellurite, and iron(III)by the moderately thermophilic bacterium Bacillus thermoamylovorans SKC1" 76 : 530-534, 2007

11 Romanenko, V.I., "Pure culture of bacteria using chromates and bichromates as hydrogen acceptors during development under anaerobic conditions" 46 : 414-417, 1977

12 Kilic, N. K., "Proteomic changes in response to chromium(VI) toxicity in Pseudomonas aeruginosa" 101 : 2134-2140, 2010

13 Soni, S. K., "Pretreatment of Cr(VI)-amended soil with chromateeducing rhizobacteria decreases plant toxicity and increases the yield of Pisum sativum" 66 : 616-627, 2014

14 Kurniawan, T. A., "Physico-chemical treatment techniques for wastewater laden with heavy metals" 118 : 83-98, 2006

15 Linos, A., "Oral ingestion of hexavalent chromium through drinking water and cancer mortality in an industrial area of Greece - an ecological study" 10 : 50-, 2011

16 Ryan, R. P., "Multiple metal resistant transferable phenotypes in bacteria as indicators of soil contamination with heavy metals" 5 : 95-100, 2005

17 Yang, Y., "Microbial removal of NOX at high temperature by a novel aerobic strain Chelatococcus daeguensis TAD1 in a biotrickling filter" 203-204 : 326-332, 2012

18 Lall, R., "Metal reduction kinetics in Shewanella" 23 : 2754-2759, 2007

19 Cheung, K.H., "Mechanism of hexavalent chromium detoxification by microorganisms and bioremediation application potential: a review" 59 : 8-15, 2007

20 Murugavelh, S., "Isolation, identification and characterization of Cr(VI) reducing Bacillus cereus from chromium contaminated soil" 230 : 1-9, 2013

21 Zahoor, A., "Isolation of Cr(VI) reducing bacteria from industrial effluents and their potential use in bioremediation of chromium containing wastewater" 21 : 814-820, 2009

22 Ilias, M., "Isolation and characterization of chromium(VI)-reducing bacteria from tannery effluents" 51 : 76-81, 2011

23 Wang, P. C., "Isolation and characterization of an Enterobacter cloacae strain that reduces hexavalent chromium under anaerobic conditions" 55 : 1665-1669, 1989

24 Xiao, X., "Identification and Hg2+-adaptation characteristics of an aerobic denitrifying bacterium" 32 : 584-589, 2012

25 Masood, F., "Hexavalent chromium reduction by Bacillus sp. strain FM1 isolated from heavy-metal contaminated soil" 86 : 114-119, 2011

26 Liang, W., "Experimental and modeling study on nitric oxide removal in a biotrickling filter using Chelatococcus daeguensis under thermophilic condition" 125 : 82-87, 2012

27 Bhattacharya, A., "Evaluation of Acinetobacter sp. B9 for Cr (VI) resistance and detoxification with potential application in bioremediation of heavy-metals-rich industrial wastewater" 20 : 6628-6637, 2013

28 Peng, L., "Evaluating simultaneous chromate and nitrate reduction during microbial denitrification processes" 89 : 1-8, 2016

29 Xu, W. H., "Enhancing effect of iron on chromate reduction by Cellulomonas flavigena" 126 : 17-22, 2005

30 Aston, J. E., "Effects of ferrous sulfate, inoculum history, and anionic form on lead, zinc, and copper toxicity to Acidithiobacillus caldus strain BC13" 29 : 2669-2675, 2010

31 Sayel, H., "Cr(VI) reduction by Enterococcus gallinarum isolated from tannery waste-contaminated soil" 62 : 1269-1277, 2012

32 Philip, L., "Cr(VI) reduction by Bacillus coagulans isolated from contaminated soils" 124 : 1165-1170, 1998

33 Jiang, W., "Cr(VI) adsorption and reduction by humic acid coated on magnetite" 48 : 8078-8085, 2014

34 Narayani, M., "Chromium-resistant bacteria and their environmental condition for hexavalent chromium removal:a review" 43 : 955-1009, 2013

35 Myers, C. R., "Chromium(VI) reductase activity is associated with the cytoplasmic membrane of anaerobically grown Shewanella putrefaciens MR-1" 88 : 98-106, 2000

36 Dogan, N. M., "Chromium(VI) Bioremoval by Pseudomonas bacteria: role of microbial exudates for natural attenuation and biotreatment of Cr(VI) contamination" 45 : 2278-2285, 2011

37 Zhang, H., "Chromium removal with cross-linked chitosan adsorption and baseprecipitation combination" 67 : 2768-2775, 2013

38 Viamajala, S., "Chromate/nitrite interactions in Shewanella oneidensis MR-1:evidence for multiple hexavalent chromium Cr(VI) reduction mechanisms dependent on physiological growth conditions" 78 : 770-778, 2002

39 Clark, D.P., "Chromate reductase activity of Enterobacter aerogenes is induced by nitrite" 122 : 233-237, 1994

40 Barrera-Diaz, C., "Chemical and electrochemical considerations on the removal process of hexavalent chromium from aqueous media" 33 : 61-71, 2003

41 Li, H., "Biosorption of Zn(II) by live and dead cells of Streptomyces ciscaucasicus strain CCNWHX 72-14" 179 : 151-159, 2010

42 Benazir, J. F., "Bioremediation of chromium in tannery effluent by microbial consortia" 9 : 3140-3143, 2010

43 Farabegoli, G., "Biological treatment of tannery wastewater in the presence of chromium" 71 : 345-349, 2004

44 Bryan, G.W., "Bioavailability, accumulation and effects of heavy metals in sediments with special reference to United Kingdom estuaries: a review" 76 : 89-131, 1992

45 Wang, Y.T., "Bacterial reduction of hexavalent chromium" 14 : 159-163, 1995

46 Ahemad, M., "Bacterial mechanisms for Cr(VI) resistance and reduction: an overview and recent advances" 59 : 321-332, 2014

47 Almasri, M.N., "Assessment and management of long-term nitrate pollution of ground water in agriculture-dominated watersheds" 295 : 225-245, 2004

48 Yang, J., "Application of cellulose acetate to the selective adsorption and recovery of Au(III)" 111 : 768-774, 2014

49 Mohan, D., "Activated carbons and low cost adsorbents for remediation of tri- and hexavalent chromium from water" 137 : 762-811, 2006

50 Kimbrough, D. E., "A critical assessment of chromium in the environment" 29 : 1-46, 1999