Proposal of micro plasma extraction device by autonomous trigger control

다국어 초록 (Multilingual Abstract)

In this paper, we report on the development of a microfluidic device, which extracts plasma from low‐volume whole blood (10 μL), and is aimed at integration with microfluidic system for micro analytical systems. The device autonomously separates blood cells and transfers the separated plasma by steady rotation. Based on the original autonomous fluidic control theory, we developed the principle of automatic timed injection of pressurizing medium into the separation chamber. This realizes the transferring of separated plasma from blood separation chamber to the other place, after separating blood cells by centrifugal force. Through the demonstration on a polydimethylsiloxane microfluidic device, we confirmed that the automatic plasma extraction was successfully implemented as designed. The efficiency of separation was evaluated on the basis of the concentrations of blood cells in samples before and after extraction, and the rate of blood cell removal was 98.5%.