조직배양 유래 자생나리 소인경의 온탕 및 저온처리가 맹아 및 비대에 미치는 효과

다국어 초록 (Multilingual Abstract)

This study was carried out to investigate the effects of hot solution soaking and chilling treatment on sprouting and enlargement of bulblets obtained through in vitro culture in Korean native lilies with ornamental values. In vitro bulblets of Lilium cernuum, L. hansonii, L. hansonii for. mutatum, L. leichtlinii, and L. tsingtauense were soaked in distilled water or 100 ㎎?L<SUP>-1</SUP> GA₃ and GA<SUB>4+7</SUB> solution maintained at 35℃ for two hours (hot water treatment) and/or exposed at 4℃ for 0, 4, or 8 weeks (chilling treatment) and then planted in plastic trays filled with media and grown in a greenhouse at 20℃ and under 16 h photoperiod. In all species, no bulblet propagated by tissue culture sprouted without chilling or hot water treatment due to dormancy. For dormancy breaking, GA<SUB>4+7</SUB> hot solution treatment increasing sprouting by 55-96%, whereas distilled water or GA was not effective in sprouting. Chilling treatment for 4 weeks induced sprouting by 50-70% in L. cernuum and L. leichtlinii, whereas 8 weeks was needed for sprouting of L. hansonii and L. hansonii for. mutatum. Combined treatment of hot water and chilling treatments synergistically promoted sprouting. Especially, in L. cernuum and L. hansonii, GA<SUB>4+7</SUB> hot solution soaking prior to chilling for 4 weeks promoted sprouting by 35-45% compared with the reverse order. Enlargement of bulblets resulted from increase in fresh weight and diameter was promoted by the treatments that increased the sprouting percentage of bulblets. Only in L. cernuum, shoots emerged from bulblets soaked in hot GA<SUB>4+7</SUB> solution or chilled at 4℃ and shoot emergence rate was highest in bulblets soaked in hot GA<SUB>4+7</SUB> solution and then chilled for 8 weeks. From these results, the most effective method for bulblet sprouting and enlargement was to soak in hot GA<SUB>4+7</SUB> solution and then chill for 4 weeks in L. hansonii, hansonii for. mutatum, and leichtlinii, and to soak in hot GA<SUB>4+7</SUB> solution and then chill for 4 weeks in L. cernuum and tsingtauense.

참고문헌 (Reference)

1 김규원, "기내에서 재생된 자생 나리 소인경의 온탕 처리 시 GA4+7 첨가에 의한 휴면 타파 기간 단축" 한국원예학회 44 (44): 744-747, 2003

2 김규원, "기내에서 재생된 나리 소인경의 신초출현 능력의 종간 차이" 한국원예학회 43 (43): 226-230, 2002

3 Kim, K.W, "Tissue culture of ornamental flowering bulbs (geophytes)" 18 : 87-169, 1997

4 Lighty, R.W, "The lilies of Korea" The Lily Year Book, Royal Hort. Soc., London 1969

5 Kim, K.S, "The effect of growth regulators, temperature and sucrose on the dormancy in Lillium speciosum bulblets cultured in vitro" 18 : 103-111, 1991

6 Isabelle, D., "The development of dormancy in bulblet of Lilium speciosm generated in vitro. II. The effect of temperature" 80 : 431-436, 1990

7 Kim, J.Y., "Production and detection of virus-free lily plants by shoot tip culture and virazole treatment of bulbils" 37 : 64-69, 1996

8 Kim, K.W., "Physiological changes before and after breaking dormancy of Lilium hansonii bulblets regenerated in vitro" 40 : 751-754, 1999

9 Stimart, D.P., "Overcoming dormancy in Lilium longiflorum bulblets produced in tissue culture" 107 : 101-107, 1982

10 Park, K.I., "Introduction and proliferation of callus in Liliium Oriental Hybrids in vitro" 41 : 297-300, 2000

11 Higgins, W.S, "Influence of in vitro generation temperature and post-in vitro cold storage duration on growth response of Lilium longiflorum bulblets" 115 : 930-933, 1990

12 Takayama, T., "Identification of gibberellins and abscisic acid in bulbs of Lilium × elegans Thunb. and their quantitative changes during cold treatment and the subsequent cultivation" 62 : 189-196, 1993

13 Stimart, D.P, "Foliar emergence from bulblets of Lilium longiflorum Thunb. as related to in vitro generation temperatures" 106 : 446-450, 1981

14 Lin, W.C., "Exogenous gibberellins and abscisic acid effects on growth and development of Lillium longiflorum" 100 : 9-16, 1975

15 Choi, S.T, "Effects of low temperature and hot water treatment to the dormant bulbs on the leaf emergences of bulblets during scale propagation of Easter lily (Lilium longiflorum Thunb.)" 24 : 42-48, 1983

16 Kim, K.W., "Effects of culture conditions on dormant status of in vitro regenerated Lilium Oriental Hybrid bulblets" 39 : 641-646, 1998

17 Woo, J.H., "Effect of growth regulators and culture method on shoot formation from microscale in Lilium Oriental Hybrids ‘Casa Blanca’" 41 : 297-300, 2000

18 Niimi, Y., "Effect of chilling and GA3 treatments on breaking dormancy in Lilium rubellum Baker bulblets cultures in vitro" 57 : 250-257, 1988

19 Kamerbeek, G.A., "Dormancy in bulbs and corms" 5 : 233-239, 1972

20 Yae, B.W., "Dormancy breaking and in vitro growth of in vitro bulblets in Lilium Oriental hybrid ‘Casa Blanca’" 42 : 99-102, 2001

21 Takayama, S, "Differentiation in Lilium bulbscales grown in vitro. Effect of activated charcoal, physiological age of bulbs and sucrose concentrations on differentiation and scale leaf formation in vitro" 48 : 121-125, 1980

22 Matsuo, E, "Differences in chilling effects in shoot emergence from the bulb and on leaf emergence from the scale bulblet in Lilium longiflorum Thunb" 14 : 68-69, 1979

23 Choi, S.T, "Cut-flower production of bulbous plants" 2 : 63-74, 1998

24 Matsuo, E., "Cultural practices influencing premature daughter leaf and/or shoot emergence in scale-propagated Easter lily" 17 : 196-198, 1982

25 김규원, "Control and manipulation of dormancy in Lilium bulblets regenerated in vitro" Yeungnam Univ. Press, Korea 136-142, 1999

26 Tsukamoto, Y, "Change of endogenous growth substances in Easter lily as affected by cooling" 23 : 75-81, 1971

27 Stimart, D.P., "Axis elongation from tissue-culture-generated bulblets of Lilium longiflorum Thunb" 108 : 99-101, 1983