최신 Single Nucleotide Polymorphism (SNP) 분석법의 원리 및 유전자 연구 응용법 = Principle And Application of Single Nucleotide Polymorphism (SNP) Analysis

다국어 초록 (Multilingual Abstract)

This mini review describes the principles, protocols, and applications of three commercially available Single Nucleotide Polymorphism (SNP) genotyping platforms, the TaqMan® SNP Genotyping Assay, SNPlexTM Genotyping System and Gene Chip Assay. These technologies meet the requirements of multiple SNP applications in genetics research including diseases based on genetic background. This article also describes a set of SNP selection guide and useful web sites for SNP informations. Overall, the TaqMan assay format is suitable for low- to mid-throughput applications in which a high assay conversion rate, simple assay workflow, and low cost of automation are desirable. The SNPlex Genotyping System, on the other hand, is well suited for SNP applications in which throughput and cost-efficiency are essential, e.g., applications requiring either the testing of large numbers of SNPs and samples, or the flexibility to select various SNP subsets. Gene Chip Assay is powerful tool to analyze whole SNPs and has benefit to find new SNPs involved diseases or phenotype but has a defect that it is costly. In the conclusion, this article suggests the appropriate methods for the desired experiments by comparing with these three SNPs technologies.

목차 (Table of Contents)

• 서론
• TaqMan® 방식의 SNP Genotyping Assay
• SNPlexTM Genotyping System
• Gene Chip Assay
• SNP Probe의 선정 및 design
• SNP analysis의 방향과 응용
• 결론
• 참고문헌

참고문헌 (Reference)

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