스포츠 재활훈련과 결합된 나노 인대가 인대손상 환자의 치료효과에 미치는 영향 = Effect of Nano-ligament Combined with Sports Rehabilitation Training on Treatment Effect of Patients with Ligament Injury

국문 초록 (Abstract)

연구목적 본고는 토끼 전십자인대(Rabbit Anterior Cruciate Ligament)가 재건된 힘줄뼈의 복원 동물 모형을 만들었다. 동시에 주사용 골형성단백질(rhBMP-2) 딜레이드-릴리스 나노캡슐(Delayed-Release Nanocapsules)이 힘줄 뼈 터널 계면의 복원 효과를 평가하였다. 이 연구는 전십자인대 손상의 임상치료에 이론적인 근거를 제공할 수 있을 것이다. 연구방법 중에 46마리의 토끼는 평균 몸무게가 3.31±0.45kg이었다. 전신마취는 토끼귀의 정맥 주사로 이뤄진다. 전십자인대를 제거한 후 옆 종골건을 채취하여 재건해 전십자인대의 동물모형으로 여긴다. BMP-2팀은 힘줄터널 경계면에 RHBMP-2 딜레이드-릴리스 나노캡슐을 주사하고 대조팀은 RHBMP-2를 투여하지 않았다. 결과 BMP-2팀과 대조팀은 BMP-2항체염색에 양성반응을 보여 염색이 비교적으로 적다. BMP-2팀은 염색이 더욱 균일하고 질서있다. BMP-2팀은 대조팀과 비교했을 때 힘줄-골 터널 경계면의 섬유세포가 현저히 증가하여 폐색점에서 힘줄의 구조가 유사하다. 결론 실험 결과 인대가 재건된 BMP-2는 힘줄 터널 계면의 유합을 촉진하는 데 중요한 역할을 하는 것으로 나타났다.

다국어 초록 (Multilingual Abstract)

PURPOSE This paper establishes an animal model of tendon-bone repair after rabbit anterior cruciate ligament reconstruction. At the same time, the paper evaluates the effect of injectable rhBMP-2 nano-controlled release capsule on the repair of tendon-bone tunnel interface, and evaluates the effect of injectable rhBMP-2 nano-controlled release capsule on the repair of tendon-bone tunnel interface. METHOD Research can provide experimental basis for clinical treatment of anterior cruciate ligament injury. Among them, 46 rabbits were included, with an average body weight of (3.31±0.45) kg. General anesthesia was performed via intravenous injection into the ear of rabbit. After the anterior cruciate ligament was resected, the contralateral Achilles tendon was taken for reconstruction, which was used as the animal model of tendon-bone repair after anterior cruciate ligament reconstruction. In the BMP-2 group, rhBMP-2 nano-controlled release capsules were injected at the tendon-bone tunnel interface during the operation; rhBMP-2 was not injected in the control group during the operation. RESULT The BMP-2 group and the control group showed positive expression of BMP-2 antibody staining, and the staining was lighter. The BMP-2 group stained more evenly and orderly. In the BMP-2 group, fibroblasts in the tendon-bone tunnel interface were significantly increased compared with the control group, and there was a similar structure of tendon at the bone stopping point. CONCLUSION Experiments showed that BMP-2 after ligament reconstruction had a significant role in promoting the healing of the tendon-bone tunnel interface.