Circular RNA hsa_circ_0030018 acts as a sponge of miR‐599 to aggravate esophageal carcinoma progression by regulating ENAH expression

다국어 초록 (Multilingual Abstract)

Esophageal carcinoma (EC) bears one of the most rapid‐growing incidences in cancers, which also has the highest mortality rate worldwide. Multiple studies have authenticated that circular RNAs (circRNAs) significantly work on the progression of cancers. circRNA hsa_circ_0030018 was also verified to exert functions on the development of glioma previously. Nevertheless, the biological function of hsa_circ_0030018 in EC has not been well elucidated yet. In the present study, the results displayed the expression of hsa_circ_0030018 was dramatically increased in EC cells. Inhibition of has_circ_0030018 suppressed cell proliferation, migration, and epithelial‐mesenchymal transition (EMT) process in EC. Based on molecular mechanism assays, has_circ_0030018 served as a sponge of miR‐599. Enabled homolog (ENAH), which exhibited high expression in EC cells, was confirmed to be a downstream target gene of miR‐599. Additionally, has_circ_0030018 positively regulated ENAH expression while miR‐599 negatively regulated ENAH expression. Finally, by employing rescue assays, ENAH deficiency partially counteracted the promoting function of miR‐599 silence on cell proliferation, migration, and EMT process in EC cotransfected with sh‐ has_circ_0030018#1 cells. In conclusion, hsa_circ_0030018 acted as a sponge of miR‐599 to aggravate EC progression by regulating ENAH expression. Therefore, hsa_circ_0030018 might serve as a promising biomarker and therapeutic target for EC.
hsa_circ_0030018 expression is boosted and silence of hsa_circ_0030018 inhibits esophageal carcinoma (EC) progression. Hsa_circ_0030018 acts as a sponge of miR‐599 in EC Hsa_circ_0030018 aggravates EC progression by sponging miR‐599 to regulate enabled homolog expression