Zic3z Defines the Dorsal and Vegetal Neuroectoderm in the Zebrafish Embryonic Development

다국어 초록 (Multilingual Abstract)

The Zic family is a group of genes encoding zincfinger proteins that are highly expressed in the mammaliancerebellum. Zic genes are the vertebrate homologue ofDrosophila pair-rule gene, odd-paired (opa), which playsimportant roles in the parasegmental subdivision as well asin the visceral mesoderm development of Drosophilaembryos. Recent studies on human, mouse, frog, fish andascidian Zic homologues support that Zic genes areinvolved in a variety of developmental processes, includingneurogenesis, myogenesis, skeletal paterning, and left-rightaxis establishment. In an effort to explore possible functionsof Zic proteins during vertebrate embryogenesis, we initialyhomologue of zic3 (zic3z). zic3z transcripts are detected inthe neuroectoderm, neural plate, dorsal neural tube, andbrain regions including eye field during early embryonicdevelopment. Marker DNA studies found that zic3ztranscription is modulated by BMP, Wnt, and Nodal signalsparticularly in the dorsal and vegetal neuroectoderm atgastrula. Interfering with zic3z translation with zic3z-specificmorpholino causes abnormal brain formation and expansionof the optic stalk cells. Retinal ganglion cells (RGCs)undergo abnormal neuronal diferentiation. These findingssuggest that zic3z defines the dorsal and vegetalneuroectoderm to specify brain formation and retinalneurogenesis during early embryonic development.

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