Monascus pilosus M - 15에 의한 콜레스테롤 합성저해제 Monacolin의 생산

국문 초록 (Abstract)

본 연구는 콜레스테롤 합성저해제인 monacolin을 생산하기 위하여 Monascus sp.에서 생산균주를 선발하고 생산조건을 검토하였다. Monascus sp. 중에서 Mon-ascus pilosus M-15에 의하여 생산되는 monacolin의 생산조건은 최적 생산 배지로는 5% glycerin, 2% glucose, 0.5% corn steep liquor, 0.5% NaNO₃, 0.2% MgSO₄ㆍ7H₂O이였으며, 최적 pH는 6.0이였고, 25℃에서 10~20일간 배양하였을 때 monacolin을 추출하여 UV.IR. 및 GC/MS로 확인한 결과 표준품인 monacolin과 거의 비슷한 경향을 나타내었다.

다국어 초록 (Multilingual Abstract)

The monacolin retard in vivo synthesis of cholestrol. This study was conducted to select the best strain of Monascus sp. for monacolin production among 21 strains. In addition, research on suitable condition for mass production was conducted. Monascus pilosus M-15 was selected as the best strain for production of monacolin. The appropriate substrate for the monacolin production seem to be as follow : glycerin 5%, glucose 2%, corn steep liquor 0.5%, sodium nitrate 0.5% and length of fermentation was at 25℃ for 10~12days, respectively. The monacolin obtained from this organism was compared with standard monacolin using UV, IR, and GC/MS system and it was very close to the standard monacolin.

목차 (Table of Contents)

• Abstract
  
• 서론
  
• 재료 및 방법
  
• 결과 및 고찰
  
• 요약
  
• 감사의 글
  
• 문헌