Fibrin Glue를 이용한 가토 연골 세포의 배양 = Cultures of Rabbit Chondrocytes Embedded in Fibrin Glue

다국어 초록 (Multilingual Abstract)

Chondrocytes isolated from rabbit articular cartilage were cultured in vitro within fibrin glue carrier (Beriplast-p) for 2 weeks. Histochemical and electronmicroscopic approaches were used to study chondrocyte behavior and phenotypic expression.
Under these culture condition, chondrocytes anssumed a rounded morphology, accumulated metachromatic matrix, and took on the cytological of in vivo cartilage cells. No signs of cytotoxic effects of fibrin glue were observed.
Most of the metachromatic synthesized matrix were deposited within the fibrin glue, and areas of matrix increased with time during first 2 weeks.
It was clear that this cell-culture system, using periplant, provides chondrocytes with a suitable environment in which accumulate synthesized matrix macromolecules and preserve their diffentiated phenotype in vitro.