토사자 추출물이 MCF-7 유방암 세포의세포자멸사에 미치는 영향 = Effects of Cuscutae Semen Water Extract on Apoptosis of MCF-7 Human Breast Cancer Cells

다국어 초록 (Multilingual Abstract)

This study aimed to evaluate the effects of Cuscutae Semen waterextract (CS) on MCF-7 human breast cancer cells. Methods: To clarify the results, we cultivated MCF-7 cells in cell culture plates. And then we extracted each of 100 μg/ml, 300 μg/ml, 600 μg/ml CS, gave it toMCF-7 cell. After these process we performed MTT assay to elucidate the ability of apoptosis. The result of mRNA was analyzed by RT-PCR. Results: Each of concentrated extracts CS decreased the survival rate of MCF-7cells. CS decreased Bcl-2 which is known as a blocking cell apoptosis. Bax, caspase-3,P21 and RIP-1 that accelerate apoptogenic activity factors increased by CS. CSdid not change the condition of caspase-8, caspase-9, P53 factors on MCF-7 cells. Furthermore caspase-8, caspase-9, P53 factors on MCF-7 cells does not make itmore active but turn it on. Conclusions: According to the above results, we could suggest that CS can occurthe apoptosis on MCF-7 cells.

참고문헌 (Reference)

1 대한한방부인과학회, "한방여성의학(하)" 도서출판 의성당 393-399, 2012

2 윤성중, "토사자에 대한 문헌적 고찰" 6 (6): 59-65, 1991

3 김봉수, "토사자류의 항산화작용에 대한 연구" 12 (12): 67-84, 1997

4 이재복, "토사자 약침의 항암작용 및 면역효과에 대한 실험적 연구" 18 (18): 94-104, 2001

5 강소신의학원, "중약대사전(하)" 상해과학기술출판사 2006-3008, 1978

6 김정진, "유방종괴에 관한 문헌적 고찰" 12 (12): 29-43, 1998

7 강진오, "유방암의 방사선 치료" 21 (21): 37-42, 2005

8 글로북스편집국, "부인과" 글로북스 220-, 2009

9 전국한의과대학 본초학 공동교재편찬위원회, "본초학" 영림사 620-622, 2006

10 유윤조, "兎絲自 煎湯液이 自然發證 高血壓 白鼠의 血壓에 미치는 影響" 15 (15): 164-168, 2001

11 Cheung CH, "Survivin - biology and potential as a therapeutic target in oncology" 6 : 1453-1462, 2013

12 Renault TT, "Regulation of Bax mitochondrial localization by Bcl-2 and Bcl-x(L): keep your friends close but your enemies closer" 45 (45): 64-67, 2013

13 도용호, "PC12 Cell에 대한 ？絲子의 안전성 및 항산화작용에 대한 연구" 대한한방부인과학회 19 (19): 121-134, 2006

14 Gunasekera RS, "Lycopene and lutein inhibit proliferation in rat prostate carcinoma cells" 58 (58): 171-177, 2007

15 김진명, "Lutein의 항산화 기능에 관한 반응속도론적 연구" 한국교원대학교 대학원 2010

16 Thapa RJ, "Interferon-induced RIP1/RIP3-mediated necrosis requires PKR and is licensed by FADD and caspases" 110 (110): 3109-3118, 2013

17 박혜민, "IPP 생합성 경로의 대사공학을 통한 대장균의 β-carotene 생산성 향상" 경상대학교 대학원 2007

18 Hand D, "Hormonal therapies for early breast cancer: systematic review and economic evaluation" 11 (11): 1-134, 2007

19 Yin H, "Free radical oxidation of cardiolipin: chemical mechanisms, detection and implication in apoptosis, mitochondrial dysfunction and human diseases" 46 (46): 959-974, 2012

20 Gunia S, "Expression of p53, p21 and cyclin D1 in penile cancer: p53 predicts poor prognosis" 65 (65): 232-236, 2012

21 Kim JY, "Effect of Cuscuta japonica on RANKL-stimulated osteoclastogeneis" 14 (14): 77-89, 2010

22 Kilbride SM, "Central roles of apoptotic proteins in mitochondrial function" 32 (32): 2703-2711, 2013

23 Olsson M, "Caspases and cancer" 18 (18): 1441-1449, 2011

24 Fiandalo MV, "Caspase control: protagonists of cancer cell apoptosis" 34 (34): 165-175, 2012

25 Cheng HC, "Carotenoids suppress proliferating cell nuclear antigen and cyclin D1 expression in oral carcinogenic models" 18 (18): 667-675, 2007

26 Kim JD, "Capsaicin can alter the expression of tumor forming related genes which might be followed by induction of apoptosis of a Korean stomach cancer cell line, SNU-1" 120 (120): 235-241, 1997

27 Tanaka T, "Cancer chemoprevention by carotenoids" 17 (17): 3202-3242, 2012

28 Kwon YS, "Antioxidative constituents from the seeds of Cuscuta shinensis" 6 (6): 135-138, 2000

29 Nam EJ, "Alteration of cellcycle regulation in epithelial ovarian cancer" 18 (18): 1169-1182, 2008

30 Chen AY, "A review of the dietary flavonoid, kaempferol on human health and cancer chemoprevention" 138 (138): 2099-2107, 2013