Aspergillus sp. LAM 94-142가 생산하는 세포응집물질의 특성 = Characterization of a Cell Aggregation Factor from Aspergillus sp. LAM 94-142

국문 초록 (Abstract)

Aspergillus sp. LAM 94-142가 생산하는 세포응집 물질의 특성은 다음과 같았다. 배양여액을 IRA 420과 IRC 120으로 처리한 후 1% NaCl을 함유한 acetone 침전 및 Sepharose 4B column chromatography로 회수율 48%로 약 15배 정제하였다. 정제된 물질은 mannose, arabinose 및 glucose가 31 : 17 : 2의 조성비로 이루어진 분자량 약 90만 dalton의 heteropolysaccharide였으며 최적 작용 pH와 온도는 각각 pH 7∼9와 40℃ 부근이었고 pH 3 이하와 9 이상에서는 불안정하였으나 열처리에 대해서는 대단히 안정하였다. 응집반응시 각종 당류는 별로 영향을 미치지 못하였으나 milk casein이나 hemoglobin 같은 polypeptide와 Hg^(2+), Fe^(2+), Cu^(2+) 등의 금속 이온은 응집활성을 저해하였다. 그리고 Bacillus, Escherichia, Pseudomonas, Staphylococcus, Sarcina 등의 세균과 Cryproccus neoformans에 대해서는 응집활성을 나타내었으나 Candida나 Saccharomyces 등의 효모에는 활성이 없었다.

다국어 초록 (Multilingual Abstract)

A cell aggregation factor produced by Aspergillus sp. LAM 94-142 was purified and partially characterized. The factor was purified about 15 folds from culture broth by IRA 420 and IRC 120 treatment, 1% NaCl added acetone precipitation, and Sepharose 4B column chromatography with overall yield of 48%. It was heteropolysaccharide consisted of mannose, arabinose, and glucose with a molar ratio, 31:17:2, and its molecular weight was estimated to be about 900,000 daltons by Sepharodse 4B gel filtration method. The optimum pH and temperature was 8 and 40℃, respectively. The factor was stable in pH range of 3∼9 and at 100℃ for 90 min. The cell aggregation activity of the factor was inhibited by the addition of Hg^(2+), Fe^(2+), Cu^(2+), and some polypeptides such as milk casein or hemoglobin. The factor aggregated Bacillus subtilis, B. macerans, B. turingiensis, E. coli. Peudomonas aeruginosa, P. fluorescens. P. malophilia, and weakly aggregated Staphylococcus sp., Sarcina lutea, P. putida and Cryptococcus neoformans, but it didn’t aggregate various strains of Candida sp. and Saccharomyces sp.