Dynamics of Mixed - Cultures of Gluconobacter suboxydans and Saccharomyces uvarum

다국어 초록 (Multilingual Abstract)

A mixed-culture of Gluconobacter suboxydans IFO 3172 and Saccharomyces uvarum IFO 0751 was peerformed in a synthetic medium. The optimal inoculum ratio of G. suboxydans and S. uvarum for mixed-culture fermentation was 150 : 1. The optimum pH, incubation temperature and aeration rate for mixed-culture fermentation were 5.0, 30℃ and 2.25vvm, respectively. As a result of batch pure- and mixed-culture fermentation, specific growth rate in pure-culture of both strain was lower than that in mixed-culture. The yield of cell mass from S. uvarum exclusively decreased. The growth rate of the mixed-culture was very similar to the pure-culture in the beginning of culture, but it has been decreased after 16hrs. In the mean time, S. uvarum in mixed-culture fermentation could grow due to fructose converted, but it could not grow in pure-culture fermentation. Thus, the relationship was a sort of commensalism. The kinetic parameters calculated through steady-state results during continuous fermentations are as follows: μ_(max1)=0.1l8(h^(-1)), Ks₁=0.330(g/L), μ_(max2)=0.162(h^(-1)), KS₂=0.038(g/L). The yield of bacterial cell mass was relatively constant, but yield of yeast cell mass was gradually decreased.

목차 (Table of Contents)

• Abstract
  
• INTRODUCTION
  
• MATERIALS AND METHODS
  
• RESULTS AND DISCUSSION
  
• REFERENCES