대전지역의 요로감염 환자로부터 분리된 요로병인성 대장균 ST131과 Non-ST131의 비교 분석 = Comparative Analysis of Uropathogenic Escherichia coli ST131 and Non-ST131 Isolated from Urinary Tract Infection Patients in Daejeon

다국어 초록 (Multilingual Abstract)

Uropathogenic Escherichia coli (UPEC) is a major cause of urinary tract infections (UTIs), which is one of the most common infectious diseases in humans worldwide. Since UPEC is increasingly gaining resistance to many antimicrobial agents, antibiotic therapy of UTI has recently become a great concern. This study examined the epidemiological relationship, and antimicrobial resistance patterns of 84 UPEC isolates obtained from UTI patients in Daejeon, from March to December 2017. Molecular epidemiology was investigated by multilocus sequence typing (MLST), and an antimicrobial susceptibility test was determined using an E-test. In this study, UTI was more common in females (73.8%) than in males (26.2%), and the highest incidence of UTI was observed in the age group in their 70s. Among the 84 UPEC isolates, 59 isolates (70.2%) were multidrug-resistant (MDR), and the major sequence type was ST131 (44 isolates, 52.4%). Interestingly, the rates of MDR in non-ST131 isolates (72.5%) were higher compared to ST131 isolates (68.2%). These results indicate the possibility of the development and spread of MDR in non-ST131 isolates. Effective surveillance networks and continuous research need to be conducted globally to prevent the emergence and international spread of MDR non-ST131 isolates.
Copyright Ⓒ 2020 The Korean Society for Clinical Laboratory Science. All rights reserved.

참고문헌 (Reference)

1 Amee R. Manges, "Widespread Distribution of Urinary Tract Infections Caused by a Multidrug-Resistant Escherichia coli Clonal Group" Massachusetts Medical Society 345 (345): 1007-1013, 2001

2 Loh K, "Urinary tract infections in pregnancy" 2 : 54-57, 2007

3 Norouzian H, "The relationship between phylogenetic groups and antibiotic susceptibility patterns of Escherichia coli strains isolated from feces and urine of patients with acute or recurrent urinary tract infection" 11 : 478-487, 2019

4 Allan Ronald, "The etiology of urinary tract infection: traditional and emerging pathogens" Elsevier BV 113 (113): 14-19, 2002

5 Betsy Foxman, "The epidemiology of urinary tract infection" Springer Science and Business Media LLC 7 (7): 653-660, 2010

6 Hosam M. Zowawi, "The emerging threat of multidrug-resistant Gram-negative bacteria in urology" Springer Science and Business Media LLC 12 (12): 570-584, 2015

7 B Foxman, "Recurring urinary tract infection: incidence and risk factors." American Public Health Association 80 (80): 331-333, 1990

8 Thomas M Hooton, "Recurrent urinary tract infection in women" Elsevier BV 17 (17): 259-268, 2001

9 Donna A Cheung, "Prevalence, co-infection and antibiotic resistance of Escherichia Coli from blood and urine samples at a hospital in Jamaica" Journal of Infection in Developing Countries 14 (14): 146-152, 2020

10 Abdulaziz Alqasim, "Prevalence and molecular characteristics of sequence type 131 clone among clinical uropathogenic Escherichia coli isolates in Riyadh, Saudi Arabia" Elsevier BV 27 (27): 296-302, 2020

11 D. J. Schwartz, "Positively selected FimH residues enhance virulence during urinary tract infection by altering FimH conformation" Proceedings of the National Academy of Sciences 110 (110): 15530-15537, 2013

12 Iranpour D, "Phylogenetic groups of Escherichia coli strains from patients with urinary tract infection in Iran based on the new clermont phylotyping method" 2015 : 846219-, 2015

13 Clinical and Laboratory Standards Institute, "Performance standards for antimicrobial susceptibility testing; twentieth informa-tional supplement" Clinical and Laboratory Standards Institute 2010

14 SeyedAbdol Reza Mortazavi-Tabatabaei, "Pattern of antibacterial resistance in urinary tract infections: A systematic review and meta-analysis" Medknow 10 (10): 169-, 2019

15 Yusuke Matsui, "Multilocus sequence typing of Escherichia coli isolates from urinary tract infection patients and from fecal samples of healthy subjects in a college community" Wiley 9 (9): 1225-1233, 2020

16 A.-P. Magiorakos, "Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance" Elsevier BV 18 (18): 268-281, 2012

17 Mukherjee M, "Multidrug-resistance and extended spectrum beta-lactamase production in uropathogenic E" 7 : 449-453, 2013

18 Laura Fernandes Gonçalves, "Multidrug resistance dissemination by extended-spectrum β-lactamase-producing Escherichia coli causing community-acquired urinary tract infection in the Central-Western Region, Brazil" Elsevier BV 6 : 1-4, 2016

19 Gisele Peirano, "Molecular epidemiology of Escherichia coli producing CTX-M β-lactamases: the worldwide emergence of clone ST131 O25:H4" Elsevier BV 35 (35): 316-321, 2010

20 Julie Plantamura, "Molecular epidemiological of extended-spectrum β-lactamase producing Escherichia coli isolated in Djibouti" Journal of Infection in Developing Countries 13 (13): 753-758, 2019

21 Kim Bongyoung, "Molecular Epidemiology of Ciprofloxacin-Resistant Escherichia coli Isolated from Community-Acquired Urinary Tract Infections in Korea" 대한감염학회 52 (52): 194-203, 2020

22 Jing Wu, "Molecular Characteristics of ST1193 Clone among Phylogenetic Group B2 Non-ST131 Fluoroquinolone-Resistant Escherichia coli" Frontiers Media SA 8 : 2294-, 2017

23 Nicolas-Chanoine MH, "Intercontinental emergence of Escherichia coli clone O25 : H4-ST131 producing CTX-M-15" 61 : 273-281, 2008

24 M.-H. Nicolas-Chanoine, "Escherichia coli ST131, an Intriguing Clonal Group" American Society for Microbiology 27 (27): 543-574, 2014

25 Belayneh Regasa Dadi, "Distribution of virulence genes and phylogenetics of uropathogenic Escherichia coli among urinary tract infection patients in Addis Ababa, Ethiopia" Springer Science and Business Media LLC 20 (20): 108-, 2020

26 da Cruz Campos, "Determining the Virulence Properties of Escherichia coli ST131 Containing Bacteriocin-Encoding Plasmids Using Short- and Long-Read Sequencing and Comparing Them with Those of Other E. coli Lineages" MDPI AG 7 (7): 534-, 2019

27 Alghoribi MF, "Antibiotic-resistant ST38, ST131 and ST405 strains are the leading uropathogenic Escherichia coli clones in Riyadh, Saudi Arabia" 70 : 2757-2762, 2015

28 Atul Kothari, "Antibiotic resistance in pathogens causing community-acquired urinary tract infections in India: a multicenter study" Journal of Infection in Developing Countries 2 (2): 354-358, 2008