흰쥐에서 Isoniazid 전투여가 halothane마취시 간에 미치는 영향에 대한 연구 = An Experimental Study of the Effect of Halothane Anesthesia on the Liver Function in Sprague-Dawley Rats Pretreated with Isoniazid

다국어 초록 (Multilingual Abstract)

The present study were performed to determine the effects of the halothane anesthesia on the liver function in Sprague-Dawley rats pretreated with isoniazid. Ninety six male Sprague-Dawley rats were divided into four groups. Saline (2m1/kg, in group Ⅰ) or isoniazid (50mg/kg, in group Ⅱ) was injected intraperitoneally for 7 days, and then exposed to sham anesthesia 24 hours after the last treatment. Saline (2ml/kg, in group Ⅲ)or isoniazid (50mg/kg, in group Ⅳ) was injected intraperitoneally for 7 days, and then exposed to 1% halothane (N_2O-O_2:3L/min-3L/min) 24 hours after the last treatment. Blood sampling was done before any treatment through tail vein. Intracardiac puncture for blood sampling was done after intraperitoneal injection of pentobarbital (50mg/kg) and hepatectomy was done to get the tissue sample for light microscopic examination 24 hour after exposure to air or halothane in one-half of the rats from each group ; the remaining half of the rats from each group were examined 96 hours after exposure to air or halothane. Biochemical studies included liver function variables (protein, albumin, total bilirubin, direct bilirubin, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase).
The results were summarized as follows ;
1. The histologic evidence of hepatic damage was more severe in group Ⅱ and group Ⅳ than Ⅰ and Group Ⅲ respectively 24 hours after exposure to air or halothane. In group Ⅳ, the histologic score revealed more severe hepatic damage than group Ⅱ.
2. The histologic evidence of the hepatic damage was more severe in group Ⅳ 96 hours after exposure halothane.
3. The biochemical study showed no significant differences among the groups.
The above results suggest that isoniazind itself can produce liver injury and exposure to halothane may aggravate the isoniazid induced hepatic injury.