This study was performed to investigate the possible effect of Lactobacillus spp. on the growth and citrinin production by Penicillium citrirtum. Lactobacillus bulgaricus and Lactobacillus casei were grown with Pen. citrinum in modified APT broth cont...
This study was performed to investigate the possible effect of Lactobacillus spp. on the growth and citrinin production by Penicillium citrirtum. Lactobacillus bulgaricus and Lactobacillus casei were grown with Pen. citrinum in modified APT broth containing 7% of glucose and incubated at 30℃ for 15 days. Four inoculation procedures were used; (a) Lactobacillus spp. and Pen. citrinum were grown alone(Pc, Lb, and Lc), (b) both organisms were added simultaneously(ST; Pc+Lb and Pc+Lc), (c) Lactobacillus spp. was grown 3 days, then conidia of Pen. citrinum were added(LbPc and LcPc), and (d) Pen. citrinum was grown 3 days, then Lactbacillus spp. was added (PcLb and PcLc). At 0, 3, 6, 9, 12, 15 days of incubation, the growth of each organism, pH and total acidity of broth, and content of citrinin were determined. Lactobacillus app. and Pen. citrinum, when grown associatively, influenced the growth of each other. It was observed that slower growth of Pen. citrinum when in the presence of Lactobacillus app. than when the mold grew alone. Production of citrinin by Pen, citrinum was markedly less in the mixed culture. No apparent growth and toxin production was observed when the Lactobacillus app. was grown 3 days, then conidis of Pen. citrinum were sdded(LbPc and LcPc). The above results indicate that another microorganism or rnmpeting microtlora in the culture can affect the behavior of Pen. citriaum.