Modulators of Ion Transport in Nasal Polyps: An in situ Measurement of Short-Circuit Current

다국어 초록 (Multilingual Abstract)

Objectives. To examine possible modulators of the ion transport through the apical membrane of the nasal polyps.
Methods. The study was conducted using the freshly-excised nasal polyps from the patients with chronic sinusitis. A voltage-
sensitive vibrating probe technique was introduced to monitor the short-circuit current across the apical membrane
of the polyp at 37℃.
Results. In the presence of amiloride, Adenosine 5’-triphosphate induced 4,4’-Diisothiocyanatostilbene-2,2’-disulfonic acidsensitive
chloride current. Uridine 5’-diphosphate was less potent than Uridine 5’-triphosphate, and adenosine increased
chloride secretion, which was blocked by the antagonist, 8-(p-sulfophenyl) theophylline on adenosine receptor. Based
on the pharmacologic profiles, multiple purinergic receptors, including P2Y2, P2Y6, and P1 receptors, were functionally
expressed. However, P2X receptor agonists ( , -methyleneadenosine 5’-triphosphate and 2’- & 3’-O-[4-benzoyl-benzoyl]
adenosine 5’-triphosphate), Cystic fibrosis conductance regulator (CFTR) activator (genistein), nitric oxide substrate
(L-arginine), and nitric oxide donor (sodium nitroprusside) had no significant effect on the short circuit current.
Conclusion. Among tested drugs, P2Y receptor agonists were major modulators of ion transport in nasal polyps in situ.

다국어 초록 (Multilingual Abstract)

Objectives. To examine possible modulators of the ion transport through the apical membrane of the nasal polyps.
Methods. The study was conducted using the freshly-excised nasal polyps from the patients with chronic sinusitis. A voltage-
sensitive vibrating probe technique was introduced to monitor the short-circuit current across the apical membrane
of the polyp at 37℃.
Results. In the presence of amiloride, Adenosine 5’-triphosphate induced 4,4’-Diisothiocyanatostilbene-2,2’-disulfonic acidsensitive
chloride current. Uridine 5’-diphosphate was less potent than Uridine 5’-triphosphate, and adenosine increased
chloride secretion, which was blocked by the antagonist, 8-(p-sulfophenyl) theophylline on adenosine receptor. Based
on the pharmacologic profiles, multiple purinergic receptors, including P2Y2, P2Y6, and P1 receptors, were functionally
expressed. However, P2X receptor agonists ( , -methyleneadenosine 5’-triphosphate and 2’- & 3’-O-[4-benzoyl-benzoyl]
adenosine 5’-triphosphate), Cystic fibrosis conductance regulator (CFTR) activator (genistein), nitric oxide substrate
(L-arginine), and nitric oxide donor (sodium nitroprusside) had no significant effect on the short circuit current.
Conclusion. Among tested drugs, P2Y receptor agonists were major modulators of ion transport in nasal polyps in situ.

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