재래종 쌀보리의 형태적 특성 및 RAPD에 의한 유연 관계 분석 = Evaluation of Morphological Characteristics and RAPD Analysis in Korean Landraces of Naked Barley

국문 초록 (Abstract)

재래종 쌀보리 들은 추파형이며 내한성이 약하고 장간이면서 밀수, 장망으로 출수가 늦은 품종이 많았다. 그러나 파성, 내한성, 출수기, 간장, 수장의 변이가 컸으며, 내한성이 강한 품종, uzu 유전자, 찰성, 자색종피 등 다양한 유용 돌연변이를 보였다. 재래종들의 유연관계를 형태적 특성과 RAPD분석 결과, 112품종 모두 다른 품종으로 밝혀졌으며, 재래종 쌀보리와 겉보리의 유전적 특성을 비교한 결과, 일양성이 인정되어 쌀보리 품종들이 겉보리 재래종으로부터 돌연변이 되었던 것으로 추정할 수 있었다.

다국어 초록 (Multilingual Abstract)

Barley varieties collected from 1940 to 1951 allover the Korean peninsula by Dr. Takahashi Ryuhei were reintroduced from the Research Institute for Bioresouces in Okayama University, Japan, and the evaluation of morphological characteristics and RAPD analysis were performed. Totally, 493 varieties of Korean barley landraces were planted in the green house, from which seeds of 491 varieties were harvested and conserved in the seedbank of the Rural Development Administration. Majority of the naked barley varieties showed dense spikes with long awn, late heading, winter habits, and long plant height. However, variants having various phenotypes such as short awn, blue aleurone color, brachytic type and waxyness were also identified. Plant height, spike length, and cold-tolerance in the varieties were also highly variable among them. Homogeneity tests on the variation of growth habits, spike density, anthocyanin pigmentation on the seed coat, and hairiness on leaf sheath between naked and covered barley showed that the variations of naked barley were similar to those of covered barley. It maybe indicate that the most of naked barley landraces were mutated from the covered barley landraces. Korean landraces of naked barley were broadly divided into 4 groups by the dendrogram produced by morphological characteristics; however, the identities of the group were rather indistinct. Many varieties, belonged to the same group, were showed different band patterns in RAPD analysis using 5 different primer sets. These results indicate that the 112 varieties of naked barley landraces were different genotypes.