High-Frequency Regeneration and Transformation of Raphanus sativus

다국어 초록 (Multilingual Abstract)

We have achieved high-frequency shoot regeneration in radish (Raphanus sativus). Cotyledon explants from four-day-old seedlings were suitable for the effective induction of shoots on Murashige and Skoog's (MS) medium containing 3.0㎎/L kinetin. We also determined that it was essential to include 1 - to 2-㎜ petiole segments with the cotyledons for efficient induction. When the regenerated shoots were transferred to an MS liquid medium containing 0.1㎎/L NAA, roots formed within four weeks, and normal plant development ensued. We established a transformation protocol using an Agrobacterium binary vector that carries the CDS reporter gene. Preculturing the explants for 1 d in an MS medium containing 3.0㎎/L kinetin also increased efficiency. Five days of cocultivation proved best for delivering T-DNA into radish. Transformation frequencies of up to 52% were obtained in shoot induction media that contained 3.0㎎/L kinetin.