Effect of partially purified sub-fractions of Pterocarpus mildbraedii extract on carbon tetrachloride intoxicated rats

다국어 초록 (Multilingual Abstract)

Background: This study was designed to determine the hepatoprotective effect of partially purified fractions from Pterocarpus mildbraedii extract on carbon tetrachloride (CCl4) intoxicated rats.
Methods: The methanol extract of P. mildbraedii was subjected to solvent partitioning using n-hexane, chloroform, ethylacetate and water. Separation of fractions with proven antioxidant activity was achieved by chromatographic techniques. Acute toxicity and hepatoprotective studies of the methanol sub-fraction 6 (Me 6), methanol sub-fraction 7 (Me 7) and methanol sub-fraction 8 (Me 8) from P. mildbraedii extract on carbon tetrachloride (CCl4) intoxicated Wister rats.
Results: Intoxication of rats with CCl4 resulted in significant (p < 0.05) increase in the activities of aspartate transferase (AST), alanine transferase (ALT), alkaline phosphatase (ALP), level of malondialdehyde (MDA) while glutathione (GSH) concentration, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities were decreased. Administration of Me6, Me7 and Me8 sub-fractions of P. mildbraedii caused a significant reduction (p > 0.05) in the activities of the liver enzymes, MDA level, total and direct bilirubin in dose-dependent manner. There were significant (p < 0.05) increases in GSH concentration, SOD, CAT, and Gpx activities in the treated groups. The subfractions also restored the damaged hepatic-architecture in the treated groups.
Conclusion: Therefore, Me6, Me7 and Me8 leaf sub-fractions of P. mildbraedii extract have hepatoprotective effect. Therefore, this vegetable can further be exploited as a source of drug/supplement development in the prevention and treatment of liver damage.

참고문헌 (Reference)

1 Sturgill MG, "Xenobiotic-induced hepatotoxicity : mechanisms of liver injury and methods of monitoringhepatic function" 43 : 1512-1526, 1997

2 Meister A, "Transport and direct utilization ofgamma-glutamylcysteine for glutathione synthesis" 80 : 707-711, 1983

3 Panda S, "Thyroid inhibitory,antiperoxidative and hypoglycemic effects of stigmasterolisolated from Butea monosperma" 80 : 123-126, 2009

4 Ruot B, "Theresponse of liver albumin synthesis to infection in ratsvaries with the phase of the inflammatory process" 102 : 107-114, 2002

5 Ogbadoyi EO, "Therapeutic evaluation of Acacia nilotica(Linn)stem barkextract in experimental African trypanosomiasis" 4 : 11-18, 2011

6 Misra HP, "The role of superoxide anion in theautoxidation of epinephrine and a simple assay forsuperoxide dismutase" 247 : 3170-3175, 1972

7 Ogbu SI, "The effects of storage temperatureprior to separation on plasma and serum potassium" 10 : 1-4, 2001

8 Ahmed AE, "Studies onthe mechanism of haloacetonitrile-induced gastrointestinaltoxicity : interaction of dibromoacetonitrile with glutathioneand glutathione-S-transferase in rats" 6 : 115-, 1991

9 Oke JM, "Screening of some Nigerianmedicinal plants for antioxidant activity using2,2-diphenyl-picryl-hydrazyl radical" 5 : 77-79, 2002

10 Wang SY, "Scavenging capacity of berry crops onsuperoxide radicals, hydrogen peroixide, hydroxyl radicalsand singlet oxygen" 48 : 677-684, 2000

11 Kumar V, "Robbins & Cotranpathologic basis of disease" SaundersElsevier 2009

12 Lieber CS, "Relationships between nutrition, alcohol use andliver disease" 27 : 220-231, 2003

13 Mayuren C, "Protective effect ofLivactine against CCl4and paracetamol inducedhepatotoxicity in adult Wistar rats" 2 : 491-495, 2010

14 Wallace HA, "Principle and method of toxicology" Raven Press 45-345, 2001

15 Ogukwe CE, "Phytochemical screening on the leaves of Sansevieriatrifasciata" 29 : 8-10, 2004

16 Hamzah RU, "Phytochemical screening and antioxidant activity ofmethanolic extract of selected wild edible Nigerianmushrooms" 4 : 153-157, 2014

17 Rabiat UH, "Peperomiapellucida in diets modulates hyperglyceamia, oxidative stressand dyslipidemia in diabetic rats" 135-140, 2012

18 OECD, "Paris, France Organisation for economic co-operation anddevelopment guidelines for acute toxicity of chemicals"

19 Owais M, "Modern phytomedicineturning medicinal plants into drugs" Wiley-VCH Verlag GmBH & Co 233-242, 2006

20 Aruoma OI, "Methodological considerations forcharacterizing potential antioxidant actions of bioactivecomponents in food plants" 523-524, 2003

21 Ijeh II, "Medicinal evaluation of Xylopiaaethiopica and Ocimum gratissimum" 26 : 44-47, 2004

22 Odediran FA, "Marketing performance of Pterocarpus Mildbreadii in Ibadanmetropolis" 60A : 16293-16295, 2013

23 Stroev EA, "Laboratory manual in biochemistry" Mir Publishers 81-114, 1989

24 Sharma N, "Inhibition ofbenzo (a) pyrene- and cyclophoshamide-inducedmutagenicity by Cinnamomum cassia" 480-481, 2001

25 Ugwu GC, "In vivo evaluation of the effects ofethanolic leaf extract of Gongronema latifolium on AST andbilirubin secretions in albino rats" 2 : 1-2, 2010

26 Okpala JC, "Igwe JC.Effects of n-butanol fraction of Gongronema latifolium leaveextract on some biochemical parameters in CCl4-inducedoxidative damage in Wistar albino rats" 8 : 52-64, 2014

27 Dominic AA, "Hypolipidemic activity of Cyperous rotundus on CCl4-induceddyslipidemia in rats" 2 : 51-53, 2012

28 Joshi BC, "Hepatoprotective potential ofantioxidant potent fraction from Urticadioica Linn. (Wholeplant) in CCl4 challenged rats" 2 : 1101-1110, 2015

29 Saleem TSM, "Hepatoprotective herbs a review" 1 : 1-5, 2010

30 Kumar KS, "Hepatoprotective effects of 50%ethanolic extract of Ficus hispida Linn against CCl4inducedhepatotoxicity in rats" 4 : 01-4, 2012

31 Abirami A, "Hepatoprotective effectof leaf extracts from Citrus hystrix and C. maxima againstparacetamol induced liver injury in rats" 4 : 35-41, 2015

32 Patrick-Iwuanyanwu KC, "Hepatoprotective effect of crude methanolic extract andfractions of Ring worm plant Senna alata leaves from Nigeriaagainst carbon tetrachloride-induced hepatic damage inrats" 1 : 128-138, 2011

33 Nevien IS, "Hepatoprotective and antioxidant effects ofSilybum marianum plant against hepatotoxicity induced bycarbon tetrachloride in rats" 8 : 4-, 2012

34 "Guide for the care and use of laboratory animals. 8th ed"

35 Grant GH, "Fundamentals of clinical chemistry" W.B.Saunders 328-329, 1987

36 Tietz NW., "Fundamental of clinical chemistry" W.B. SaundersCompany 1020-1038, 2000

37 Ramadan A, "Evaluation of the safety and antioxidantactivities of Crocus sativus and Propolis ethanolic extracts" 16 : 13-21, 2012

38 Varshney R, "Effects of Calmodulin antagonist onradiation induced lipid peroxidation in microsomes" 58 : 733-744, 1990

39 Nkosi CZ, "Effect of pumpkin seed(Cucurbita pepo) protein isolate on the activity levels ofcertain plasma enzymes in CCl4-induced liver injury in lowprotein fed rats" 19 : 341-345, 2005

40 Adebayo JO, "Effect of ethanolic extract of Khaya senegalensis on somebiochemical parameters of rat kidney" 88 : 69-72, 2003

41 Akanji MA, "Effect of chronicconsumption of metabisulphite on the integrity of rat livercellular system" 81 : 173-179, 1993

42 Tietz NW, "Clinical guide to laboratory tests" W.B.Saunders Company 17-, 1976

43 Rutherford AE, "Chronic hepatitis: hepatitis: Merck manualprofessional [Brochure]"

44 Donia AM, "Chemical constituents and protectiveeffect of Ficus ingens (Miq.) on carbon tetrachloride-inducedacute liver damage in male Wistar albino rats" 17 : 125-133, 2013

45 Akpanyung EO, "Chemical composition ofthe edible leaves of P. mildbraedii" 48 : 209-215, 1995

46 Adaramoye OA, "Changes in antioxidant statusand biochemical indices after acute administration ofartemether, artemether-lumefantrine and halofantrine inrats" 102 : 412-418, 2008

47 Chavan SD, "Biochemical andhistopathological studies of Butea monosperma(Lam)Taubleaves on paracetamol-induced hepatotoxicity in albinorats" 5 : 4006-4008, 2012

48 World Health Organization, "Basic OECD principles of GLP" World Health Organization

49 Mahlo1 SM, "Antioxidant andantifungal activity of selected plant species used intraditional medicine" 7 : 2444-2450, 2005

50 Erukainure OL, "Antilipemic and hypolipidemic activities ofGlobimetula braunii" 63 : 657-661, 2011

51 King PRM, "Alkaline phosphatase method" 7 : 322-, 1976

52 Halliwell B, "Aging, neurological and mental disorders" Springer-Verlag 21-40, 1992

53 Ramaiah SK, "A toxicologist guide to the diagnosticinterpretation of hepatic biochemical parameters" 45 : 1551-1557, 2007

54 Kumar CH, "A reviewon hepatoprotective activity of medicinal plants" 2 : 501-515, 2011

55 Reitman S, "A colorimetric method for thedetermination of serum glutamic oxalacetic and glutamicpyruvic transaminases" 28 : 56-63, 1957

56 Jendrassic L, "A colorimetric method for thedetermination of serum bilirubin level" 297 : 81-, 1938