組織培養 技術을 利用한 竹柏蘭의 無菌發芽와 大量 繁殖에 關한 硏究 = Asymbiotic Germination and Mass propagation of Cymbidium lancifolium Using Tissue Culture Technique

국문 초록 (Abstract)

本實驗은 東洋蘭의 一種인 竹柏蘭(Cymbidium lancifolium)의 種子發芽를 위한 適合한 培地와, 發芽하여 生育된 根莖의 器官分化에 미치는 生長調節物質 및 活性炭 等의 影響을 究明하여 竹柏蘭의 大量繁殖을 위한 基礎資料를 얻기 위하여 實施한 結果는 다음과 같다.
1. 供試한 6種의 培地中에서 MS培地에 peptone 3g/ℓ을 添加한 것이 發芽數를 增加시켰다.
2. 發芽促進을 위한 KOH 處理는 0.1N 濃度에서 90分 處理가 發芽數가 가장 많았다.
3. 液體培地에서가 個體培地보다 그리고 暗培養하는 것이 明培養하는 것보다 發芽가 促進되었다.
4. 根莖培養의 경우 peptone 3g/ℓ을 添加한 것이 生體重과 器官分化를 促進시켰으며, 生長調節物質의 處理效果는 NAA 濃度가 BA濃度보다 높은 때는 不定根의 發生이 많았고, BA가 NAA보다 높을 때는 不定芽 發生이 많은 傾向을 보였다.
5. 物質添加 處理에서는 活性炭 2g/ℓ을 添加한 것에 바나나 100g/ℓ와 감자 50g/ℓ을 첨가하는 것이 生體重과 個體發生을 增加시켰다.
6. 여러가지 東洋蘭系統의 混植培養에서 相互間에 個體發生이 促進되었으나, 寒蘭과 竹柏蘭間에는 寒蘭의 生育은 沮害되었으나, 竹柏蘭은 器官分化와 全體的인 生育이 가장 良好하게 나타났다.

다국어 초록 (Multilingual Abstract)

This experiment was carried out in order to choose the optimal basal medium and to study the seed germination of oriental orchid, Cymbidium lancifolium and the effect of plant growth regulators and various substance treatments on organogenesis of the rhizome were also examined.
1. Germination was identified to be the best in MS+peptone medium among 6 different kinds of basic media.
2. Dipping seeds in 0.1N KOH for 90min. was most effective for promoting the germination.
3. Dark condition promoted significantly the grmination rate in liquid MS with 3g/ℓpeptone, compared with other light conditions.
4. Application of growth regulators for the MS medium had no effect on total growth of rhizome. However the medium containing 3g/ℓpeptone not only increased fresh weight, but also propmoted organogenesis during rhizome culture. The higher the BA concentration relative to NAA, the more the shoot differentiation accured, whereas the higher the NAA to BA, the more the root differentiation.
5. The more effectiveness for the rhizome growth and shoot formation was produced when 2 grams of activated charcoal, 100 grams of banana, and 50 grams of potato were added to 3 grams-peptone MS.
6. Overall, mixed culture with some kinds of oriental Cymbidium rhizome showed mutual competition in terms of nutrition absorption. This promoted a significant growth of rhizome. However, the mixed culture resulted in no mutual competition between Cymbidium kanran and Cymbidium lancifolium, rater showed that unknown material produced from C. Kanran promoted C. lancifolium to growth and organ differentiation.