Differential Manifestation of Ocular Phenotypes in TALEN-mediated p19arf Knockout FVB/N and C57BL/6J mouse lines

Jin‑Sung Park,Joo‑Il Kim,Hyun‑Jin Lim,Soo‑Kyung Ryu,Euna Kwon,Kang‑Min Han,Ki‑Taek Nam,Han‑Woong Lee,Byeong‑Cheol Kang 한국유전학회 2020 Genes & Genomics Vol.42 No.9

Background p19arf, primarily known as a tumor suppressor, has also been reported to play an essential role in normal development of mouse eyes. Consistently, lack of p19arf has been associated with ocular defects, but the mixed background of the knockout (KO) mouse strain used raised a concern on the accuracy of the phenotypes observed in association with the targeted gene due to genetic heterogeneity. Object We carried out a study to investigate into the efect of genetic background on the manifestation of p19arf KO associated phenotypes. Methods We characterized the phenotypes of novel p19arf KO mouse lines generated in FVB/N and C57BL/6J using a transcription activator-like efector nuclease (TALEN) system in comparison to the reported phenotypes of three other p19arf-defcient mouse lines generated using homologous recombination. Results Ninety-fve percent of FVB/N-p19arf KO mice showed ocular opacity from week 4 after birth which worsened rapidly until week 6, while such abnormality was absent in C57BL/6J-p19arf KO mice up to the age of 26 weeks. Histopathological analysis revealed retrolental masses and dysplasia in the retinal layer in FVB/N-p19arf KO mice from week 4. Besides these, both strains developed normally from birth to week 26 without increased tumorigenesis except for a subcutaneous tumor found in a C57BL/6J-p19arf KO mouse. Conclusion Our fndings demonstrated surprisingly variable manifestation of p19arf-linked phenotypes between FVB/N and C57BL/6J mice, and furthermore between our mouse lines and the established lines, indicating a critical impact of genetic background on functional study of genes using gene targeting strategies in mice.

Consumer Recognition Survey for Establishing the Reasonable Expiration Dates of Health Functional Foods

( Soo Jin Youn ),( Joo Youn Park ),( Hee Su Kim ),( Eun Jin Kang ),( Kil Jin Kang1 ),( Hye Kyung Park ),( Gun Hee Kim ) 한국식생활문화학회 2009 Food Quality and Culture Vol.3 No.2

This study investigated consumers` recognition on shelf life of health functional foods to set up their rational expiration date. For this study 107 male and female adults aged 20 to 59 years were randomly selected as major consumers of health functional foods. And their data were analyzed with SPSS 17.0. The questionnaire included questions asking a consumer`s recognition on the shelf life of health functional foods and how to treat health function food passing its shelf life. In this study, the consumers were found to pay attentions considerably to the shelf life. But most of them did not know an accurate meaning of the shelf life of health functional food and mistook it for expiry date. In addition, the consumers doubted safety of health functional foods passing the shelf life. Therefore, education for consumers about an accurate meaning of the shelf life was needed. The results of this study were considered to be helpful to set up a reasonable expiration date for health functional food.

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

B 형 간염 에 있어서 IgM anti HBc 의 발현도 및 임상적 의의

김경희,강진경,문영명,송경순,김용범,최흥재,황용 대한내과학회 1986 대한내과학회지 Vol.30 No.1

Testing for IgM antiHBc was useful to detecting the patients with negative and positive HBsAg viral hepatitis and may improve serodiagnostic accuracy when acute NANB and delta agent hepatitis occur in previousely unrecognized HBsAg carriers. Moreover, it may be a useful test in defining potentially high risk sources of exposure to hepatitis B virus. But it is not useful in distinguishing recent from remote infection because IgM antiHBc occasionally was found in chronic liver diseases, IgM antiHBc was found by ELISA in 32 patients with acute viral hepatitis B, 61 with chronic hepatitis B, 18 patients with liver cirrhosis, 36 healthy HBsAg carriers and l49 patients with positive antiHBc alone. We continuously evaluated HBsAg and liver function of the patients who had negative IgM antiHBc acute hepatitis. The results are summarized as follows: 1) IgM antiHBc was positive in 84.4% of acute hepatitis B, 4% of chronic hepatitis B, 5.5% of positive HBsAg liver cirrhosis, 2. 8% of HBsAg healthy carriers but was all negative in patients with positive antiHBc alone. 2) Three patients with negative HBsAg acute viral hepatitis was diagnosed by positive IgM antiHBc as acute viral hepatitis R. 3) During the follow-up of 5 patients with negative IgM antiHBc acute hepatitis, 2 patients were diagnosed as type B acute viral hepatitis because of disappearance of HBsAg and normalization of liver function. And another 3 patients were diagnosed as non-B acute viral hepatitis superimposed on HBsAg carrier or natural course of chronic hepatitis.

죽음에 관한 주관성 연구

정혜경(Hae Kyung Chung),김경희(Kyung Hee Kim),윤은자(Eun Ja Yeun),류은정(Eun Jung Ryu),염순교(Soon Gyo Yeoum),정연강(Yeun Kang Chung),권혜진(Hye Jin Kwon) 한국정신간호학회 1999 정신간호학회지 Vol.8 No.1

The purpose of this study is, by using Q-methodology, to classify the type of attitude for the general public about the death and to understand the specific characters of each type. Q-population was investigated by referring to relevant records and interviewing with the general public and the experts. The final 40 of Q-sample was selected and the data was collected through P-sample, randomly chosen 32 people around Seoul and Kyung-gi area. After analyzing, 4 types were found. Type 1 is aiming for the future life. They believe firmly the future life. They feel certain that eh death means starting for eternal life rather than being afraid of the death or having a despair. Type 2 is valuing reality. They do their best for the reality of life. They take up a positive attitude toward completing their life with responsibilities even at the situation of confronting death have a right to know when they will be dead. They should have enough time to prepare for death. Type 4 is devoting for society. Even though there is only one life, people think that devoting their life for justice is very important. Through the result of this study, people`s agreement for nobility and importance of life were found same. It is reconfirmed that instead of being dead suddenly, if it`s possible, they want to know the fact of dying to have time to prepare for their death. Also, the fact that everyone has the fear of death is confirmed.

특수교육 교육과정 국제동향 분석

강경숙,김진숙,정해진,황윤한 국립특수교육원 2004 연구보고서 Vol.- No.2

본 연구는 세계 여러 국가의 특수교육 교육과정의 개발 및 운영에 대한 정보를 수집하여 국제동향을 분석하기 위해 시행되었다. 해외 9개국과 국내의 특수교육 교육과정 개발 및 운영에 대한 동향을 살펴보고, 일반교육과정과 특수교육 교육과정의 연계성을 탐색하여 국제비교하여 향후 우리나라 특수교육 교육과정 수립 시 필요한 방향성에 대한 시사점을 도출하고자 하는 것이다. 제1장에서는 연구의 필요성 및 목적, 연구의 문제 및 내용, 연구방법. 연구의 제한점을 비롯한 연구의 개요를 기술하였다. 연구의 내용으로는, 첫째 특수교육 교육과정의 전체 교육적인 맥락을 파악하기 위해 각 국가의 교육제도 및 일반교육과정에 대한 개요를 파악하고. 둘째 특수교육 교육과정의 국가수준ㆍ지역수준ㆍ학교수준 개발에 대한 실태를 파악하고, 셋째 특수교육 교육과정의 운영 및 실제에 대한 실태를 분석하고, 넷째 특수교육 교육과정의 개발 운영에 대한 국제동향을 권역별로 비교ㆍ분석하고 시사점을 도출하여 향후 교육과정 개발을 위한 방향성을 모색하는 것이다. 이처럼 전반적인 교육제도와 일반교육과정을 함께 연구한 것은 특수교육 교육과정을 연구하는 특수교육관련 전문가만이 아니라 일반교육 교육과정 관계자와 교사들에게도 유용한 정보를 제공할 수 있을 것이며 일반 교육과정과의 관계성 정립을 통해 장애학생의 교육과정적 통합을 위한 토대를 구축할 수 있을 것이다. 연구방법 및 절차로는 우선, 일반교육과정 및 특수교육 교육과정 관련자료를 분석하여 동향 분석 준거를 설정한 후 외국의 한국교육원, 해외 거주 박사과정 및 해외연구원을 활용하여 국외 특수교육 교육과정 개발 및 운영측면에 대한 자료를 수집하였다. 또한, 해외 현지외국인 교사 및 관리자를 통한 설문조사와 면담조사, 국내 교육과정 운영자료를 수집 하기 위해 설문조사와 현장방문 및 면담조사를 실시하였다. 제2장에서는 선행연구 및 특수교육 교육과정 고찰을 위해 첫째, 교육과정 국제동향비교 관련 선행연구 고찰, 둘째 기존 특수교육과정의 변천 및 제7차 교육과정 고찰, 셋째 특수교육과정 개발 및 운영에 대한 내용을 소개하였다. 제3장부터는 각 장을 구성하고 있는 본 연구의 주요부분으로 특수교육 교육과정 국제동향에 대해 미국, 캐나다, 영국, 독일, 호주, 뉴질랜드, 일본, 중국, 홍콩을 중심으로 자료를 조사하였으며, 한국에 대해서는 교육과정 개발 및 운영 면에 대해 특정 교육청으로부터 수집한 자료와 특정 학교의 사례를 중심으로 기술하였다. 본 연구의 큰 주제는 특수교육 교육과정의 개발 및 운영이지만, 특수교육 교육과정은 그 나라의 교육제도 및 일반교육과정과 밀접한 연관성을 지니고 유기적으로 개발ㆍ운영되기 때문에 교육 전반적인 맥락을 알아보기 위해 각 국가의 교육제도, 일반교육과정의 개발 및 운영, 특수교육의 개관에 대해 먼저 살펴보았다. 본 연구의 주 핵심인 특수교육 교육과정의 개발과 운영은 면담 및 설문자료를 토대로 했을 뿐 아니라, 사진 및 교실구조도와 같은 교실현장의 자료를 토대로 특수교육의 장면 특히, 통합장면의 교육사례를 중심으로 구성하였다. 위 국가에서 연구된 내용을 중심으로 연구결론에서는 권역별로 나누어 특수교육 교육과정을 중심으로 국가간 비교를 하였다. 비교할 내용의 틀로는 첫째, 특수교육의 방향 및 관련법규, 특수교육 서비스전달체제와 같은 특수교육개관, 둘째, 특수교육 교육과정 개발 측면으로 특수교육 교육과정의 결정방식, 국가ㆍ지역ㆍ학교 수준의 교육과정 개발, 표준 및 가이드라인, 교육과정철학ㆍ목표ㆍ내용ㆍ평가 등 교육과정의 요소, 학급의 교육과정 운영 시 상위수준 교육과정과의 연계성 여부, 교육과정 적용 절차 및 시기, 교육과정 재구성에 대한 교사의 자율성 및 의사결정에 대한 국가간 비교를 하였다. 셋째, 특수교육 교육과정 운영 측면으로는 교사의 교육과정 운영에 대한 자율성 부여정도, 교육과정적 통합을 위한 일반교사와 특수교사의 협력, 일반교육과정으로의 접근 혹은 연계성, 개별화교육 계획과의 연계 측면, 교육방법과 교재활용과 같은 내용을 권역별로 비교하였다. 각 국가간 권역별 비교를 통해 향후 특수교육 교육과정 수립 및 일반교육과정과의 연계를 위한 시사점 및 제언을 도출하였다. 본 연구에서는 특히 거스를 수 없는 추세인 통합교육을 위해 어떻게 일반교육과정과 연계를 맺어야 하는지, 교사 간 협력을 어떻게 이뤄가야 하는지에 대해 초점을 맞추어 시사점을 도출해내었다. 각 국가의 특수교육 교육과정을 조사한 결과를 토대로 시사점을 도출하여 간단히 정리해보면 다음과 같다. 첫째, 세계 각국의 교육과정은 나름대로 국가수준ㆍ지역수준ㆍ학교수준의 교육과정 체제의 틀을 갖추고 있지만, 중앙 집중적이기보다는 지방 분권화되어가는 경향을 강하게 보이고 있다. 미국, 캐나다, 독일과 같은 경우는 각 주에서 독자적으로 개발한 교육과정에 따라 독창적으로 운영되고 있었다. 영국, 뉴질랜드, 중국, 일본, 우리나라의 경우는 국가교육 과정의 틀에 따라 교육과정이 구성되어 전달된다고 볼 수 있다. 특수학급의 경우 각국 공통적인 현상은 국가수준의 일반교육과정과 특수교육 교육과정이 있는 경우에도, 특수학급을 위한 학교교육과정 개발을 찾기 어려웠다. 특수학급을 위한 교육과정 개발은 지역수준 교육과정 지침에 의해 학급교육과정, 개별화지도계획 등으로 교사에게 일임되어 있는 상태이다. 둘째, 교육과정 개발 및 운영에 대한 교사의 자율권을 상당부분 제공하고 있다. 미국과 영국, 캐나다, 호주는 교육과정의 기준(standards)과 성취수준을 제시할 뿐 구체적인 교육내용이나 방법을 제시하고 있지는 않기 때문에 교사의 자율권을 상당부분 허용하고 있었으며, 독일은 주 수준에서 교육과정 내용을 제시한다 하더라도 구속력은 거의 없었다. 영국은 교사들의 교육과정 구성능력이 중시된 국가로서 교사가 스스로 개발ㆍ운영하는 데 무리가 없는 국가라고 자처하지만, 장애학생의 교육성과가 교사의 질에 전적으로 의존해야 하는 상황은 신중하게 검토해야 할 부분이다. 셋째, 전 세계적인 특수교육이 분리교육에서 통합교육의 방향으로 이동해 감에 따라 일반교육과정을 근간으로 특수교육 교육과정이 구성되어 운영되는 국가가 많았다. 캐나다, 영국, 홍콩, 호주, 뉴질랜드와 같이 유럽, 미주 지역과 그 영향권 하의 학교중심 교육과정 개발의 전통이 강한 나라들이 그러하다. 일본, 중국, 우리나라와 같이 기본교육과정이라는 특수교육 교육과정이 별도로 개발되어 있는 국가는 찾아보기 어려웠다. 즉, 많은 국가에서 일반교육과정으로의 접근, 혹은 일반교육과정과 특수교육 교육과정과의 연계성 측면을 상당부분 모색하고 있는 것으로 사료된다. 넷째, 특수교육 요구학생의 개별적인 필요에 맞게 고안된 교육과정이라 할 수 있는 개별화된 교육계획을 구성하여 운영하고 있었다. 미국, 캐나다, 영국, 호주, 뉴질랜드, 우리나라에서는 특수교육 요구학생의 교육과정이 IEP에 의해 이루어지도록 제도화되어 있었다. 미국과 캐나다에서는 학생의 학업 및 사회적 기능에 초점을 맞추어 IEP가 개발되고 있는 것으로 나타났다. 영국에서는 일반학생의 교육과정을 특수교육 요구학생의 수준에 맞게 수정ㆍ보완하거나 현저한 차이가 있는 경우, 다른 것으로 대체할 수 있도록 하였다. 다섯째, 일반교육과 특수교육의 팀 형성 및 교사간 협력관계를 토대로 특수교육 교육과정이 운영되는 것을 주목할 수 있었다. IEP를 구성할 때도 일반교사와 함께 논의하고, 학급운영에 있어서도 보조원의 지원을 받을 수 있을 뿐 아니라 교사간 팀 교수를 통해 통합 장면에서의 수업이 진행되는 것을 볼 수 있었다. 독일의 경우, 특수학교에 적을 둔 특수교육 대상학생이 일반학급에 통합되어 특수교사가 함께 일반학급에서 수업을 지도하였다. 홍콩은 통합교육을 국가주도적인 정책 방향으로 삼아 '전체학교접근'을 시도하였는데, 이는 학교전체적인 차원에서 협력하여 통합교육을 지지하는 것이라 할 수 있다. 이제 통합장면에서의 특수교육 교육과정의 운영은 협력을 떼어서는 생각할 수 없는 주제처럼 생각된다. 이 외, 영국에서는 일반학교에 특수교사 대신 특수교육코디네이터(SENCO)를 두고 있으며, 일본에서도 최근 이러한 유형의 코디네이터를 학교에 배치할 것을 계획하고 있는 것으로 나타났다. 이는 일반교육 안에서 특수교육이 함께 가기 위한 노력의 일환으로 보인다. 이처럼 일반교육과 특수교육의 일원화된 체제를 지향하기 위해 독일의 특수교사 양성 교육과정에서는 교육학 일반 및 일반교과가 특수교육 전공보다 많았으며, 홍콩과 같이 일반 교사 및 특수교사 양성체제가 이원화되어 있지 않고 일반교육을 교육받은 후 이를 토대로 특수교육을 전공하거나 연수를 통해 특수교육을 이수하게 하는 경우는 처음부터 일반교육 과정과 특수교육과정이 별개로 운영되지 않을 수 있다는 단초를 제공한다. 그리고 특수학교에서의 통합교육을 모색하고 있는 중국의 경우, 지역사회에서 특수교육적 자료를 제공 하고 상담하는 리소스센터로 거듭나기 위해 학교를 개방하고 있었는데, 통합장면만이 아닌 특수학교에서의 교육과정의 개발 및 운영도 일반교육의 그것과 더불어 함께 논의되어야 한다는 점을 강하게 시사한다. 끝으로, 본 연구에서는 향후 일반교육과정과의 연계성을 고려한 특수교육 교육과정의 개발 및 운영을 위해 일반교육과정의 개정 작업 시 특수교육전문가가 참여할 것을 제안하는데, 이는 경도장애학생의 경우 당초부터 특수교육 요구학생으로 낙인되지 않고 구제될 수 있도록 모든 학생을 고려한 보편적인 교육과정을 개발하는데 특수교육적 고려를 포함 할 수 있기 때문이다. 교육의 내용 뿐 아니라 교육방법 면에서도 차별화된 교수법 등의 제안으로 일반학급에서 장애학생이 통합되어 교육과정이 운영될 수 있도록 다양한 노력이 요구된다. The purpose of this study was to analyze international trends of development and management of special education curriculum in nine countries. The results of this study will help establish direction for the development of special education curriculum in Korea. Chapter 1 describes the needs for and purposes of this research, research questions, research methods, and limitations of this study. Sections on each country have the following structure : the education system and general education curriculum ; the development of special education curriculum at the national, local, and school level ; the management of special education curriculum and current practices in local schools: and implications for establishing direction for the development of special education curriculum. This study will serve professionals in both general education and special education field since it emphasized the perspective by which special education was viewed as part of the general education system. The focus was on investigating the relationship between general education curriculum and special education curriculum. This study attempted to establish foundation for curricular integration of students with disabilities. In terms of research methods and procedures, first, researchers developed a framework for the analysis of international trends based on literature review. Second, researchers collected data on the development and management of special education curriculum through local research assistants from the countries. Third, for the nine countries, school teachers were surveyed and interviewed on special education curriculum. Chapter 2 reviews existing literature on international trends of curriculum, current special education curriculum, and the history of the development of special education curriculum. Also information on the development and management of special education curriculum in Korea was provided. In the following chapters, data on special education curriculum from nine countries including USA, Canada, UK, Germany, Australia, New Zealand, Japan, China, and Hong Kong were analyzed. Particularly, for Korea, the description centered around the analysis of data from a District Office and case studies of local schools. Main topic of this study was the development and management of special education curriculum. However, special education curriculum can not be discussed without considering the context of general education system and curriculum. Therefore, first, reviewed were education system in general and development and management of general education system for the nine countries. The development of special education curriculum was presented at the national, local, and school level, and management of special education curriculum was described based on interview data, survey responses, pictures, and case studies. In the results section, international data on special education curriculum was compared and contrasted by the region. The framework for the comparison include (1) special education overview including special education trends, law and policy, special education service delivery system, (2) the development of special education curriculum including curriculum decision-making process, the national, local, and school level curriculum, standard and guideline, curriculum philosophy, elements of curriculum such as objectives, contents, methods, and assessment, link to the upper level curriculum, curriculum implementation procedures, teacher autonomy in curriculum restructuring, (3) the management of special education curriculum including teacher autonomy in the management of curriculum, collaboration between special education and general education teachers, link to general education curriculum, link to IEP, instructional methods, and use of text books. Based on the regional comparisons among countries, implications and suggestions were drawn. In particular, the focus was on how to connect general education curriculum with special education curriculum, and how to encourage collaborative relationships between teachers. The implications are as follows. First, although curriculum in the countries had different levels of curriculum such as the national, local, and school-level curriculum, the international trends moved toward localization from centralization. Countries including USA, Canada, Germany implemented the state-developed curriculum while countries such as UK, New Zealand, China, and Korea developed curriculum within the framework of the national level curriculum. With regard to curriculum in special education classrooms, reviewed countries appeared to share a similar trend. That is, even in the cases that had the national-level general and special education curriculum, the national-level curriculum for students in special education classrooms were not found. In most cases, curriculum for students in special education classrooms was often developed by teachers based on local curricular guidelines. Second, autonomy in the process of the development and management of curriculum was provided for teachers. For example, in USA, UK, and Canada, standards and achievement objectives were presented in the curriculum leaving out contents and instructional methods. In Germany, even though curriculum was presented at the state level, it was not mandatory. In UK where teachers' ability of curriculum development was traditionally valued, careful considerations were needed : because outcomes of students with special education needs solely depended on teacher quality. Third, as the international trends of special education moved toward inclusive education, more countries developed special education curriculum based on general education curriculum. For example, countries such as Canada, UK, Hong Kong, Australia, and New Zealand did not have separate special education curriculum at the national level. Many countries appeared to exert their effort to integrate special education into general education. Fourth, individualized curriculum for students with special education needs were used in many countries including USA, UK, Canada, Australia, New Zealand, and Korea. In USA and UK, curriculum for students with special education needs tended to focus on academics and social function, while in UK, general education curriculum was accommodated to meet the needs of students, or alternative curriculum was used in case the academic gap was too large. Fifth, it was noted that the management of special education curriculum was based on the collaborative relationship between general education teachers and special education teachers. It was observed that the collaboration was in place in the development of IEP, support of teacher aides, and co-teaching. In Germany, students enrolled in special education schools were integrated in general education classes, and special education teachers from the special school taught in the general education classroom. In case of Hong Kong, a national policy called 'whole school access' was tried in which inclusion was supported at the whole school level. That is, the management of special education curriculum can not be successful without collaboration among educators. In U.K, there was a SENCo, special education needs coordinator instead of special education teacher in general education schools. This system was scheduled to be in place in Japanese schools. This seemed to be an effort to integrate special education into general education. Another effort could be found in Germany where special education teacher training programs included more general education courses than special education courses. In Hong Kong, the teacher education systems were not even separated. That is, teachers who have completed course requirements for acquiring general education teacher certificate can major special education or attend to special education in-service to be special education teachers. This system appeared to provide head start for the integrated education system. In case of China where inclusive education in special education schools were considered, educators strongly indicated that the development and management of curriculum for students enrolled in special education schools should also be discussed. Finally, this study suggests special education professionals participate into the process of the general education curriculum revision in order to develop and manage special education curriculum that is well-connected with general education curriculum. By doing this, the development of universally designed curriculum can be empowered by widening the range of audience the curriculum covers. This, in turn, will eliminate the needs for the identification of students with mild disabilities since the universally designed general education curriculum would meet these students' needs. Successful implementation of curriculum for students with disabilities in inclusive settings requires consistent efforts from all stakeholders in education.

국내에서 파상풍의 연령별 면역혈청학적 역학연구

강진한,허재균,김종현,이경일,박수은,마상혁,이명숙,반상자,홍성화,조대현,이성호 대한감염학회 2001 감염 Vol.33 No.2

Background : The incidence of tetanus in adults and neonatal tetanus have been markedly reduced by world-wide use of DTP vaccines. But, tetanus is still one kind of major health problems in many developing countries, and several serosurvey stduies in developed ountries revealed that substantial proportions of adult opulation may lack immunity against tetanus and imunity level against tetanus is continuously decreasing by age. In Korea, tetanus outbreaks have been disappeared since the 1980s by high acceptant DTaP vaccination rates. Annually, few tetanus patient has been reported since 1990s. But, there knave been no seroepide-miological studies to tetanus, no trials to assess tetanuts immunizations. And we do not use Td vaccine in adults for maintaning tetanus immunity. In this aspect, we conducted age related survey of immunity to tetanus and indirectly assessed the immunogenecity of tetanus vaccines, used in Korea. Methods : For the evaluation of age related sero-survey of tetanus immunity in Korean population, study subjects were classified into 16 groups (A∼J group; below 10 years with one year interval, K∼O group; 11~60 yrs with 10 years interval, p group; over 60 yrs). The numbers of each group were 100, and sex distributions of each group were almostly equal. And far the indirect assessment of tetanus immunization in Korean children, children under 15 years old age were classified into 6 groups (I∼VI) according to the status of DTaP vaccination. The numbers of this each group were 50, and sex ratio was almostly equal. Specific IgG antibody to tetanus toxin were detected by ELISA. And the ANOVA repeated t-test was used to compare antibody levels in study groups. Results : In age related groups, the antibody levels to tetanus toxin were well maintained until 20 years old age group (L group), but thereafter the titers abruptly decreased below 0.1 IU/mL and over 75% populations among the groups over 30 years old age needed maintenance of protective immunity to tetanus. The antibody level of male was statistically higher than that of female in P group. In the groups related DTaP vaccination status, the antibody titer was very low in pre-vaccination group (I), but the titers after primary vaccinations were sharply increased and highly maintained until 15 years. Conclusion : The results of our study revealed that the immunity to tetanus was dramatically decreased in age groups over 30 years old. This result indicates that Td vaccination program in adults should be considered for maintenance of immunity to tetanus. And our study indicate that DtaP vaccination programs and vaccines, used in Korea, are effective for acquisition and maintenance of tetanus immunity in Korean children. (Korean J Infect 33:104~111, 2001)

共同住宅團地 外部空間의 活性化와 團地內 犯罪被害經驗率의 關係에 대한 硏究

강석진,이경훈 대한건축학회 2004 대한건축학회논문집 Vol.20 No.2

The purpose of this study is to investigate about the effect of outdoor space activation on the experienced crime victimization rate through comparison between the activated and non-activated outdoor space in Multi-Family Housings. In order to fulfill this objectives, this study was processed as follows : first, the analyses the results of questionnaire survey through the simple comparative statistical analyses among the variables such as the socio-demographic factors, the psycho-behavioral factors, the utilization rate and satisfaction of outdoor space and experienced crime victimization rate last 5-years in the present dwellings. ; second, analyses of the correlations among all of the variables included the physical site planning factors. ; third, comparing analyses the difference of the variables between the Multi-Family Housings along to the utilization rate in outdoor space. The result revealed that the activation of outdoor space through the physical site planning factors such as the number of households, building coverage, available green space rate and psycho-behavioral factors such as neighborhood relations, the cognitive life environmental fields, and positive territorial attitude would effect on the decrease of experienced crime victimization rate in the Multi-Family Housing.

임부의 연령에 따른 보완대체요법에 관한 인식 태도 경험 비교 연구

김연진,김지연,노경민,신혜리,유예지,이지민,장희조,정아인,최소정,강숙정,이향연,민혜영 이화여자대학교 간호과학대학 2016 이화간호학회지 Vol.- No.50

Purpose: This study aims to test if the age of pregnant women affects the perception, experience, and attitude towards complementary alternative therapy (CAT), by investigating the perception, experience, and attitude of pregnant women under the age of 35, and above 35 (Advanced Maternal Age). The study will 1) find the general characteristics of pregnant women under and over the age of 35 2) compare the perception, behavior, and experience of pregnant women under and over the age of 35, and 3) find the comparison of the perception, behavior, and experience according to the general characteristics of pregnant women under and over the age of 35. Method: The subjects of this study are composed of 44 women under the age of 35 and 44 women above the age of 35, totaling 88 pregnant women visiting midwiferies, obstetrics and gynecology, and baby fairs in Seoul and Gyeong-Gi area. Data for this study was collected from November 28, 2015 to January 6, 2016 for 40 days. The collected data analyzed mean, standard deviation, t test, ANOVA, and chi-square by using SPSS 23.0. Result Results show that there is a significant difference in the perception, attitude, and experience of the two-sample groups in the use of CAT. The question of “what is most necessary when applying CAT in a hospital clinical environment” for the perception criteria came out with the results of t=9.980, p<.05. For the attitude, results showed that the answers of pregnant women under the age of 35 displayed a more positive attitude towards CAT. (t=2.421, p<.05) Lastly, in the experience section, pregnant women under the age of 35 showed more CAT use than the other sample group. Conclusion Results have shown that there is a difference in the perception, behavior, and experience between the two sample groups. Therefore further research on the efficacy and the adverse effects of CAT needs to be done.

구강 편평세포암종에서 P16ink⁴유전자의 Methylation에 대한 연구

강진원,김경욱,류진우,김창진 대한악안면성형재건외과학회 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.2

The p16 protein is a cyclin dependent kinase inhibitor that inhibits cell cycle progression from G1 phase to S phase in cell cycle. Many p16 gene mutations have been noted in many cancer-cell lines and in some primary cancers, and alterations of p16 gene function by DNA methylation have been noticed in various kinds of cancer tissues and cell-lines. There have been a large body of literature has accumulated indicating that abnormal patterns of DNA methylation (both hypomethylation and hypermethylation) occur in a wide variety of human neoplasma and that these aberrations of DNA methylation may play an important epigenetic role in the development and progression of neoplasia. DNA methylation is a part of the inheritable epigenetic system that influences expression or silencing of genes necessary for normal differentiation and proliferation. Gene activity may be silenced by methylation of up steream regulatory regions. Reactivation is associated with demethylation. Although evidence or a high incidence of p16 alterations in a variety of cell lines and primary tumors has been reported, that has been contested by other investigators. The precise mechanisms by which abnormal methylation might contribute to carcinogenesis are still not fully elucidated, but conceivably could involve the modulation of oncogene and other important regulatory gene expression, in addition to creating areas of genetic instability, thus predisposing to mutational events causing neoplasia. There have been many variable results of studies of head and neck squamous cell carcinoma(HNSCC). This investigation was studied on 13 primary HNSCC for p16 gene status by protein expression in immunohistochemistry, and DNA genetic/epigenetic analyzed to determine the incidence, the mechanisms, and the potential biological significance of its Inactivation. As methylation detection method of p16 gene, the methylation specific PCR(MSP) is sensitive and specific for methylation of any block of CpG sites in a CpG islands using bisulfite-modified DNA. The genomic DNA is modified by treatment with sodium bisulfate, which converts all unmethylated cytosines to uracil(thymidine) . The primers designed for MSP were chosen for regions containing frequent cytosines (to distinguish unmodified from modified DNA), and CpG pairs near the 5' end of the primers (to provide maximal discrimination in the PCR between methylated and unmethylated DNA) The two strands of DNA are no longer complementary after bisulfite treatment, primers can be designed for either modified strand. In this study, 13 paraffin embedded block tissues were used, so the fragment of DNA to be amplified was intentionally small, to allow the assessment of methyla-tion pattern in a limited region and to facilitate the application of this technique to samlples. In this 13 primary HNSCC tissues, there was no methylation of p16 promoter gene (detected by MSP and automatic sequencing). The p16 protein-specific immunohistochemical staining was performed on 13 paraffin embedded primary HNSCC tissue samples. Twelve cases among the 13 showed altered expression of p16 proteins (negative expression). In this study, The author suggested that low expression of p16 protein may play an important role in human HNSCC, and this study suggested that many kinds of genetic mechanisms including DNA methylation may play the role in carcinogenesis.