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Lee, Deuk Yeon,Choi, Jae Hong,Shin, Jung Chul,Jung, Man Ki,Song, Seok Kyun,Suh, Jung Ki,Lee, Chang Young Elsevier 2018 APPLIED SURFACE SCIENCE - Vol.443 No.-
<P><B>Abstract</B></P> <P>Compared with wet processes, dry functionalization using plasma is fast, scalable, solvent-free, and thus presents a promising approach for grafting functional groups to powdery nanomaterials. Previous approaches, however, had difficulties in maintaining an intimate sample-plasma contact and achieving uniform functionalization. Here, we demonstrate a plasma reactor equipped with a porous filter electrode that increases both homogeneity and degree of functionalization by capturing and circulating powdery carbon nanotubes (CNTs) via vacuum and gas blowing. Spectroscopic measurements verify that treatment with O<SUB>2</SUB>/air plasma generates oxygen-containing groups on the surface of CNTs, with the degree of functionalization readily controlled by varying the circulation number. Gas sensors fabricated using the plasma-treated CNTs confirm alteration of molecular adsorption on the surface of CNTs. A sequential treatment with NH<SUB>3</SUB> plasma following the oxidation pre-treatment results in the functionalization with nitrogen species of up to 3.2 wt%. Our approach requiring no organic solvents not only is cost-effective and environmentally friendly, but also serves as a versatile tool that applies to other powdery micro or nanoscale materials for controlled modification of their surfaces.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Our plasma reactor enables uniform functionalization of powdery nanomaterials. </LI> <LI> The reactor is equipped with a porous filter electrode and a gas-blower. </LI> <LI> The porous electrode captures powdery materials and functionalizes them by plasma. </LI> <LI> The sample can be repeatedly circulated via vacuum-filtration and gas-blowing. </LI> <LI> Sample circulation increases the degree and the homogeneity of functionalization. </LI> </UL> </P>
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Genome-wide structural variation by different types of ionizing irradiation sources
Soon-Jae Kwon,Hong-Il Choi,Jung Eun Hwang,Injung Jung,Sung Min Han,Jin-Baek Kim,Joon-Woo Ahn,Sang Hoon Kim,Yeong Deuk Jo,Si-Yong Kang,Dong-Sub Kim 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
To define whole genome-level of structural variation by ionization energies and radiation doses in plant, the seeds of Ilpum rice cultivar were acutely irradiated with gamma rays (100Gy, 200Gy, and 400Gy) and ion-beams (20Gy, 40Gy, and 80Gy), respectively. Six M1 rice plants were re-sequenced by Hi-Seq2500 with Ilpum cultivar as control. The average sequencing coverage of the individuals was 10.6X, and the average mapping rate to the rice reference genome (IRGSP-1.0) sequence was 96.95%. The individual plants were irradiated with gamma-400Gy and ion-50Gy had highest variation of SNP with 471,837 and 469,147, respectively. The number of insertion/deletion was 77,500 and 77,106, the synonymous and frame-shift were 7,859 and 7,763 in above two individuals. Although high genome variation shown between Ilpum cultivar and irradiated individuals, there were non-correlation between number of variation and radiation doses. However, five individuals, except ion-20Gy, showed 33 common variant blocks (CVBs) spanning 6 Mb in whole rice genome (1.6%). The CVBs were distributed on 12 rice chromosomes, Chromosome 6 had biggest CVB (5 blocks, 1.3Mb), whereas chromosome 9 had smallest CVB (0.01Mb). Total five hundred fifty one genes were in CVBs which can regard radiation sensitive genes or may be regarded as radiation hot spots in rice genome. This study will contribute to the improvement of the radiation mutation breeding research in genetic and genomic aspect.
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