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Dongwan Kim,Junhyeon Jeon,Minseo Kim,Jinuk Jeong,Young Mok Heo,Dong-Geol Lee,Dong Keon Yon,Kyudong Han Korea Genome Organization 2023 Genomics & informatics Vol.21 No.4
Accurate and efficient microbial diagnosis is crucial for effective molecular diagnostics, especially in the field of human healthcare. The gold standard equipment widely employed for detecting specific microorganisms in molecular diagnosis is quantitative real-time polymerase chain reaction (qRT-PCR). However, its limitations in low metagenomic DNA yield samples necessitate exploring alternative approaches. Digital PCR, by quantifying the number of copies of the target sequence, provides absolute quantification results for the bacterial strain. In this study, we compared the diagnostic efficiency of qRT-PCR and digital PCR in detecting a particular bacterial strain (Staphylococcus aureus), focusing on skin-derived DNA samples. Experimentally, specific primer for S. aureus were designed at transcription elongation factor (greA) gene and the target amplicon were cloned and sequenced to validate efficiency of specificity to the greA gene of S. aureus. To quantify the absolute amount of microorganisms present on the skin, the variable region 5 (V5) of the 16S rRNA gene was used, and primers for S. aureus identification were used to relative their amount in the subject's skin. The findings demonstrate the absolute convenience and efficiency of digital PCR in microbial diagnostics. We suggest that the high sensitivity and precise quantification provided by digital PCR could be a promising tool for detecting specific microorganisms, especially in skin-derived DNA samples with low metagenomic DNA yields, and that further research and implementation is needed to improve medical practice and diagnosis.
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An Efficient Scheme for User-controlled Attribute Sharing in Federated Identity
Dongwan Shin,Rodrigo Lopes,Seok-Woo Jang 한국산학기술학회 2012 SmartCR Vol.2 No.2
One of the most challenging problems of online user activities is privacy, and various methods to implement the user-controlled release of content and attributes have been studied to address this problem. In this paper, we present a novel and efficient approach to enabling controlled access to, and selective sharing of, sensitive user attributes in federated identity management (FIM), which has been considered to be a fundamental component to facilitate a better user experience in online activities. Our approach is motivated by the notion of user centricity, which essentially means giving users a larger degree of control over their attributes. We discuss the design of a security system based on the usage of authenticated dictionary-based credentials. In order to show the feasibility of the system, we discuss a proof-of-concept implementation and conduct a performance analysis.
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African swine fever: Etiology, epidemiological status in Korea, and perspective on control
Dongwan Yoo,Hyunil Kim,Joo Young Lee,유한상 대한수의학회 2020 Journal of Veterinary Science Vol.21 No.2
African swine fever (ASF), caused by the ASF virus, a member of the Asfarviridae family, is one of the most important diseases in the swine industry due to its clinical and economic impacts. Since the first report of ASF a century ago, ample information has become available, but prevention and treatment measures are still inadequate. Two waves of epizootic outbreaks have occurred worldwide. While the first wave of the epizootic outbreak was controlled in most of the infected areas, the second wave is currently active in the European and Asian continents, causing severe economic losses to the pig industry. There are different patterns of spreading in the outbreaks between those in European and Asian countries. Prevention and control of ASF are very difficult due to the lack of available vaccines and effective therapeutic measures. However, recent outbreaks in South Korea have been successfully controlled on swine farms, although feral pigs are periodically being found to be positive for the ASF virus. Therefore, we would like to share our story regarding the preparation and application of control measures. The success in controlling ASF on farms in South Korea is largely due to the awareness and education of swine farmers and practitioners, the early detection of infected animals, the implementation of strict control policies by the government, and widespread sharing of information among stakeholders. Based on the experience gained from the outbreaks in South Korea, this review describes the current understanding of the ASF virus and its pathogenic mechanisms, epidemiology, and control.
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The Assessment of Content in EFL Compositions
Dongwan Cho(조동완) 한국외국어교육학회 2007 Foreign languages education Vol.14 No.4
This study explored how instructors teaching in the same EFL program rated content in compositions. Twenty-one writing samples written on three different topics were rated by five teacher-raters. The transcribed comments made by the teacher-raters showed that 1) their rating behaviors varied to a great extent as to the ways of commenting on and viewing the content of the compositions, 2) when a composition failed to address the topic given, it negatively affected the assessment of the quality of writing, and 3) the teacher-raters made fewer comments on the content of the formal essays than on the content of e-mails. A couple of suggestions are presented with respect to the necessity of establishing written guides for rating criteria for students" essays and of rater training.
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