hCG 단일클론 항체의 생산 및 특성 연구

최상훈,강혜경,윤도영,최규삼,최명자,최인성,정태화 ( S . Choi,H . Kang,D . Yoon,K . Choi,M . Choi,I . Choe,T . Chung ) 생화학분자생물학회 1989 BMB Reports Vol.22 No.2

Pure subunits of human chorionic gonadotropin (hCG) were obtained by chromatographic separation using DEAE-Sephadex A-25 and Sephadex G-75 for the immunization. The spleen cells obtained after immunization of either w-hCG or β-subunits were fused with myeloma cells and twelve hybridoma cell lines were established. The results showed that the fusion efficiency and antibody producibility varied depending on the myeloma cell lines. The cell line P3×63-Ag8.653 was found to be a better fusion partner than SP2/0 for the production of antibodies from hybridoma cells. For the formation of ascitic fluid in mice, Incomplete Freund`s Adjuvant(IFA) was a better priming agent than pristane. The yield of specific monoclonal antibodies by the immuno-affinity purification method was 1.6-3.4 ㎎/㎖ ascitic fluid. Seven clones were studied for the specificity to w-hCG and its subunits using ^(125)I-labeled tracer and the clones were classified into 3 groups. The equilibrium affinity constants of the seven antibodies ranged from 5×10^7 M^(-1) to 8×10^8 M^(-1).

Production and Characterization of Monoclonal Antibodies Specific to human Chorionic Gonadotropin and its Subunits

최상훈,강혜경,윤도영,최규삼,최명자,최인성,정태화,Choi, S.,Kang, H.,Yoon, D.,Choi, K.,Choi, M.,Choe, I.,Chung, T. 생화학분자생물학회 1989 한국생화학회지 Vol.22 No.2

hCG subunit를 순수 분리정제하여 w-hCG와 ${\beta}$-subunit를 각각 Balb/c 생쥐에 면역 주사하여 얻은 비장세포를 myeloma 세포와 융합하여 12개의 하이브리도마 세포주를 얻었다. Myeloma 세포주에 따른 세포융합의 효율과 hCG 항체 생성율을 조사했을 때 $P3{\times}63$-Ag 8.653이 SP2/0 보다 우수했으며, priming agent에 따른 복수생성율을 조사했을 때 IFA가 pristane 보다 우수함을 알았다. 하이브라도마 클론에서 유발된 복수액을 immuno-affinity 정제했을 때 특이 항체의 양은 클론에 따라 복수 ml 당 1.6-3.4 mg을 얻을 수 있었으며 모두 $IgG_1$으로 나타났다. 이 중 7개의 단일클론 항체클론에 대해 w-hCG 및 ${\alpha}$와 ${\beta}$-subunit에 대한 친화항수를 조사했을 때 $5{\times}10^7/M$에서 $8{\times}10^8/M$로 나타났으며 이 특이 친화성에 의해 항체를 3개의 그룹으로 분류할 수 있었다. Pure subunits of human chorionic gonadotropin (hCG) were obtained by chromatographic separation using DEAE-Sephadex A-25 and Sephadex G-75 for the immunization. The spleen cells obtained after immunization of either w-hCG or ${\beta}$-subunits were fused with myeloma cells and twelve hybridoma cell lines were established. The results showed that the fusion efficiency and antibody producibility varied depending on the myeloma cell lines. The cell line $P3{\times}63$-Ag8.653 was found to be a better fusion partner than SP2/0 for the production of antibodies from hybridoma cells. For the formation of ascitic fluid in mice, Incomplete Freund's Adjuvant(IFA) was a better priming agent than pristane. The yield of specific monoclonal antibodies by the immuno-affinity purification method was 1.6-3.4 mg/ml ascitic fluid. Seven clones were studied for the specificity to w-hCG and its subunits using $^{125}I$I-labeled tracer and the clones were classified into 3 groups. The equilibrium affinity constants of the seven antibodies ranged from $5{\times}10^7M^{-1}$ to $8{\times}10^8M^{-1}$.

The application of computer program for determination of fluid properties and P-T condition from microthermometric data on fluid inclusions

오창환(Chang whan Oh),최상훈(Sang hoon Choi) 대한자원환경지질학회 1993 자원환경지질 Vol.26 No.1

유체포유물은 광상의 기원과 물리화학적인 환경 연구에 널리 사용되어져 왔다. 하지만 측정치의 오차와 유체포유물의 밀도와 isochore 등의 계산에 있어 그 과정의 복잡성 때문에 유체포유물에 내포한 물리화학적인 정보를 정확히 알아내는 것은 매우 힘들다. HALWAT, CO₂ 그리고 CHNACL 등의 컴퓨터 프로그램들은 (Nicholl and Crowford, 1985) 유체포유물의 밀도와 isochore 등을 복잡한 공식을 이용하여 계산함으로서 유체포유물로 계산될 수 있는 물리화학적인 정보의 정확도를 개선하였다.본 논문에서는 이들 프로그램을 백악기에 형성된 금학 광산에 대한 최상훈과 소칠섭 (1992)의 유체포유물 측정치와 쥬라기에 형성된 삼황학 광산에 대한 윤성택 (1990)의 유체포유물 측정치에 적용하여 보았다. 컴퓨터 프로그램을 이용하여 결정된 금학광산의 온도-압력조건은 230°~290℃, 500~800 bar로서 최상훈과 소칠섭 (1992)에 의하여 추정된 온도-압력조건인 280~360℃, 500~800 bar와 유사하다. 하지만 본 연구에서 결정된 삼황학 광산에 대한 온도-압력 조건은 대략 4~7 kb, 328±50°~344±55℃이며 이는 윤성택 (1990)에 의하여 추정된 온도-압력 조건인 1.2~1.9 kb, 255°~294℃와 차이를 보여준다. 삼황학 광산에서의 온도-압력 추정에 있어서의 차이는 광상의 온도-압력조건 추정에 있어 균일화 온도와 유체포유물의 포획온도를 동일시하고 적당치 못한 isochore의 사용함에 기인한다. 금학광상의 경우는 낮은 압력조건 (<1~2kb)에서 균일화 온도와 유체포유물의 포획온도가 비슷하기 때문에 큰 차이를 보여 주지 않았다. 따라서 1~2 kb보다 높은 압력조건에서 형성된 광상에서는 광상의 온도-압력조건 추정에 있어 균일화 온도의 잘못된 적용과 적당치 못한 isochore의 사용을 피하여야 하며 이를 위하여 본 연구에서 소개된 컴퓨터 프로그램 이용을 추천한다. Fluid inclusion has been widely used to study the origin and physiochemical conditions of ore deposits. However, it is difficult to get the accurate physiochemical data from fluid inclusion study due to the error of microthermometric data and the complexity of calculation of density and isochore of fluid inclusion. The computer programs HALWAT, CO₂, and CHNACL written by Nicholls and Crowford (1985) partly contributed to improve the accuracy of physiochemical data by using complicated equations. These programs are applied to determine the densities and isochores of fluid inclusions for the Cretaceous Keumhak mine using Choi and So’s data (1992) and for the Jurassic Samhwanghak mine using Yun’s data (1990). The estimated P-T for Keumhak mine from calculated isochores of coexisting fluid inclusions are 230°~290℃ and 500~800 bar which matche well to the P-T estimated by Choi and So (280°~360℃ and 500~800 bar, 1992). However, the P-T for Samwhanghak mine estimated in this study by combining the calculated isochores and sulfur isotope geothermometer data by Yun (1990) are about 4~7 kb at 329±50°~344±55℃ which are quite different form the P-T estimates by Yun (255°~294℃ and 1.2~1.9 kb, 1990). This discrepancy caused by misinterpretation of homogenization temperature (Th) of fluid inclusion and by application of inappropriate isochores. The application of homogenization temperature and/or inappropriately selected isochore to determine the trapping P-T condition of ore-deposits should be avoided, particularly for ore-deposits formed at pressures higher than 1~2 kb.

A Study on Hybrid Image Coder Using a Reconfigurable Multiprocessor System (Study II : Parallel Algorithm Implementation

최상훈,이광기,김인,이용균,박규태,Choi, Sang-Hoon,Lee, Kwang-Kee,Kim, In,Lee, Yong-Kyun,Park, Kyu-Tae The Institute of Electronics and Information Engin 1993 전자공학회논문지-B Vol.b30 No.10

Motion picture algorithms are realized on the multiprocessor system presented in the Study I. For the most efficient processing of the algorithms, pipelining and geometrical parallel processing methods are employed, and processing time, communication load and efficiency of each algorithm are compared. The performance of the implemented system is compared and analysed with reference to MPEG coding algorithm. Theoretical calculations and experimental results both shows that geometrical partitioning is a more suitable parallel processing algorithm for moving picture coding having the advantage of easy algorithm modification and expansion, and the overall efficiency is higher than pipelining.

The Geochemistry of Copper-bearing Hydrothermal Vein Deposits in Goseong Mining District (Samsan Area), Gyeongsang Basin, Korea

최상훈,소칠섭,권순학,최광준,Choi, Sang Hoon,So, Chil Sup,Kweon, Soon Hag,Choi, Kwang Jun The Korean Society of Economic and Environmental G 1994 자원환경지질 Vol.27 No.2

Copper-bearing hydrothermal vein mineralization of the Samsan area was deposited in two stages (I and II) of quartz-calcite-sulfide veins which fill fissures in Cretaceous volcanic and sedimentary rocks of the Gyeongsang basin. The major ore minerals, chalcopyrite and sphalerite, together with pyrite, galena, hematite, and minor sulfosalts, occur with epidote and chlorite as gangue minerals in stage I quartz veins. Chlorite geothermometry, fluid inclusion and stable isotope data indicate that copper ore was deposited mainly at temperatures between $330^{\circ}C$ and $280^{\circ}C$ from fluids with salinities between 12 and 3 equiv. wt % NaCl. Evidence of fluid boiling indicates a range of pressures from ${\leq}100$ to 200 bars bars. Within ore stage I there was an apparent decrease in ${\delta}^{34}S$ values of $H_{2}S$ with paragenetic time, from 8.0 to 2.3 per mil. This pattern was likely achieved through progressive increases in activity of oxygen accompanying boiling and mixing. In the early part of the first stage, the high temperature, high salinity fluids gave way to progressively cooler and more dilute fluids of the late parts in the first stage and of the second stage. There is a systematic decrease in calculated ${\delta}^{18}O_{water}$ values with decreasing temperature in the Samsan hydrothermal system, from values of -86 per mil for early portion of stage I through -5.9 per mil for late portion of stage I to -6.3 per mil for stage II. The ${\delta}D$ values of fluid inclusion waters also decrease with paragenetic time from -76 per mil to -86 per mil. These trends combined with mineral paragenesis and fluid inclusion data are interpreted to indicate progressive cooler, more oxidizing meteoric water inundation of an early exchanged meteoric hydrothermal system.

Morphology, Mineralogy and Genetic Implication of Placer Gold from the Huongkhe Area, Vietnam

최상훈,최선규,한진균,Choi, Sang-Hoon,Choi, Seon-Gyu,Han, Jin-Kyun The Korean Society of Economic and Environmental G 1996 자원환경지질 Vol.29 No.3

Placer gold in collected heavy minerals from several localities in Huongkhe area, is consistently very finegrained (${\leq}100$ to $400{\mu}m$). The size and size distribution show somewhat differences at Dongdo and Hoahai : at Dongdo, predominant relatively larger and wide distribution; at Hoahai, characteristic relatively finer and narrow distribution range. The morphology of gold grains is divided into the four groups assumed by the dimension ratio : spherical, subprismoidal, prismoidal, and irregular. The gold grains at Dongdo show wide morphological distribution, whereas, at Hoahai, spherical form is predominant (${\approx}75%$). Three main types of gold are classified based on their chemical composition and mode of occurrence: type I (electrum; fineness=568~931), type II (amalgam; fineness=671~927), and type III (native gold; fineness=923~999). Type I gold contains, relatively high and variable silver contents (${\approx}11$ to 58 atomic % Ag), and has been classified into two subtypes based on their silver contents (type IA, ${\approx}11{\sim}39$ atomic % Ag; type IB, ${\approx}40{\sim}58$ atomic % Ag). However, type I gold would have been generally original compositions of electrum which originated at the provenance deposits. Mercury reacts with gold and silver to form amalgam (type II gold) which has variable Hg contents (1.2~30.5 atomic % Hg). The mercury contents in gold grains at Hoahai (10.9~30.5 atomic % Hg) are higher than those at Dongdo (5.8~21.1 atomic % Hg). The gold grains from the area generally exhibit a high-purity gold (type III) rim. The individual rims on the various grains range from <1 to $80{\mu}m$ in thickness and have silver contents of <10 atomic percent Ag, even though the core compositions range from ${\approx}11$ to 58 atomic percent Ag. The rim of gold most likely is responsible for the commonly cited cases of gold from placer deposits assaying at higher values of fineness than the gold in the corresponding source lode. The gold-rich rim in the Huongkhe area apparently forms by a combination of self-electrorefining and preferential dissolution of silver under oxidizing nature during the weathering and transport process. All data of gold grains in the Huongkhe area suggest that the transport distances and/or time of placer gold at Hoahai are generally farther than those at Dongdo. The mercurian gold bearing provenance deposits at Dongdo and Hoahai would be suggest nearest epithermal gold-silver vein-type.

Geochemical Environments of Copper-bearing Ore Mineralization in the Haman Mineralized Area

최상훈,Choi, Sang-Hoon The Korean Society of Economic and Environmental G 2009 자원환경지질 Vol.42 No.1

The Haman mineralized area is located within the Cretaceous Gyeongsang Basin along the southeastern part of the Korean peninsula. Almost all occurrences in the Haman area are representative of copper-bearing polymetallic hydrothermal vein-type mineralization. Within the area are a number of fissure-filling hydrothermal veins which contain tourmaline, quartz and carbonates with Fe-oxide, base-metal sulfide and sulfosalt minerals. The Gunbuk, Jeilgunbuk and Haman mines are each located on such veins. The ore and gangue mineral paragenesis can be divided into three distinct stages: Stage I, tourmaline + quartz + Fe-Cu ore mineralization; Stage II, quartz + sulfides + sulfosalts + carbonates; Stage III, barren calcite. Equilibrium thermodynamic data combined with mineral paragenesis indicate that copper minerals precipitated mainly within a temperature range of $350^{\circ}C$ to $250^{\circ}C$. During early mineralization at $350^{\circ}C$, significant amounts of copper ($10^3$ to $10^2\;ppm$) could be dissolved in weakly acid NaCl solutions. For late mineralization at $250^{\circ}C$, about $10^0$ to $10^{-1}\;ppm$ copper could be dissolved. Equilibrium thermodynamic interpretation indicates that the copper in the Haman-Gunbuk systems could have been transported as a chloride complex and the copper precipitation occurred as a result of cooling accompanied by changes in the geochemical environments ($fs_2$, $fo_2$, pH, etc.) resulting in decrease of solubility of copper chloride complexes.

비대칭형 스크류 로터용 플라이커터의 치형설계에 대한 연구

최상훈,Choi, Sang-Hun 대한기계학회 1997 大韓機械學會論文集A Vol.21 No.1

In this study, we designed tooth profile of the fly-cutter for antisymmetric rotor which is used in screw compressor. In order to verify this profile, we manufactured three different pairs(J46, N46, P46) of antisymmetric rotor using fly-cutter. We got the following conclusions from this study. (1) We obtained better contact condition using 3pairs of rotor which are manufactured by the fly-cutter. (2) We could prevent the cutter interference near bottom point of the robe of screw rotor.

자전연소 합성법을 이용한 W-B 화합물 합성 및 조건 변수의 영향

최상훈,원창환,Choi, Sang-Hoon,Nersisyan, Hayk,Won, Changwhan 한국재료학회 2014 한국재료학회지 Vol.24 No.5

Due to their unique properties, tungsten borides are good candidates for the industrial applications where certain features such as high hardness, chemical inertness, resistance to high temperatures, thermal shock and corrosion. In this study, conditions were investigated for producing tungsten boride powder from tungsten oxide($WO_3$) by self-propagating high-temperature synthesis (SHS) followed by HCl leaching techniques. In the first stage of the study, the exothermicity of the $WO_3$-Mg reaction was investigated by computer simulation. Based on the simulation experimental study was conducted and the SHS products consisting of borides and other compounds were obtained starting with different initial molar ratios of $WO_3$, Mg and $B_2O_3$. It was found that $WO_3$, Mg and $B_2O_3$ reaction system produced high combustion temperature and radical reaction so that diffusion between W and B was not properly occurred. Addition of NaCl and replacement of $B_2O_3$ with B successfully solved the diffusion problem. From the optimum condition tungsten boride($W_2B$ and WB) powders which has 0.1~0.9 um particle size were synthesized.

5.8GHz DSRC 노변기지국용 안테나 모듈

최상훈(Sanghoon Choi),이원조(WONJO LEE),조항기(Hangki Cho),나희경(Heukyong Na),김상국(Sangkook Kim),권대혁(Daehyuk Kwon),천동필(Dongpil Chun) 한국자동차공학회 2003 한국자동차공학회 Symposium Vol.2003 No.8

An integrated antenna module for RSE in DSRC system is proposed, designed, and manufactured. A waveguide slot array antenna with strip dipoles is used and an analytical technique is applied for the fast design of the array antenna. An RF module is integrated into the waveguide assembly. The results show that the antenna module conforms to the DSRC standards with advantage of cost and size.<br/>