약제내성과 종양세포크기의 다양성

정해원,최진영,이원영 대한보건협회 1988 대한보건연구 Vol.14 No.1

Drug resistance of P 815 mouse tumor cells after various drug treatment were studied "by scoring the changes of cell sizes. Percent of cells with abnormal large cell size (larger than 15㎛. in diameter) increased by treatment of anticancer drugs. When the cells were treated with adriamycin, cells larger than 15㎛ in diameter were observed at second day post-treatment and the percent of larger cells reached maximum at 9th day post-treatment. Similar trends were observed following the treatment with cisplatin, 5-Fu, or methotrexate. Inverse relationship between survival of tumor cell population and percent distribution of large cells was observed when cisplatin was treated. Thus it was suggested that co-existence of abnormal large cells and cells in original size in the tumor cell population after drug treatment might be a key to determine that the cell line is resistant which thereby indication for next drug of choice.

말티즈견에서 발생한 파종성혈관내응고를 동반한 거미막하 출혈 증례

정해원,이희천,문종현,정동인 한국임상수의학회 2014 한국임상수의학회지 Vol.31 No.4

An 11-year-old male Maltese dog was presented with sudden onset of convulsion and right sided circling. On neurological examination, left side proprioception and menace reflexes were delayed. Blood examinations indicatedsevere thrombocytopenia and increased hepatic enzymes. On brain magnetic resonance imaging, lesions were foundedon the left lateral subarachnoid space area. Those lesions showed hyperintense on T1-weighted images, hyperintenseon T2-weighted images and hyperintense on fluid attenuated inversion recovery images. Cerebrospinal fluid analysisrevealed xanthochromia and erythrophagocytosis. Coagulation test results demonstrated that fibrin degradation productand D-dimer concentrations were higher than normal range. The patient expired few hours after presentation. Thiscase report demonstrates intracranial hemorrhage with disseminated intravascular coagulation in a dog.

치주질환 병인균의 길항작용과 bacteriocin 유사물질의 특성에 관한 연구

정해원,정종평,손성희 서울大學校 齒科大學 1988 齒大 論文集 Vol.12 No.1

광전송기술의 표준화동향

정해원,조성준,김재근 한국통신학회 1996 정보와 통신 Vol.13 No.12

초고속 액세스 망의 모델화 및 경제성에 대한 검토

정해원,박기식,노장래,조성준 한국통신학회 1997 한국통신학회논문지 Vol.22 No.12

It is generallyaccepted that broadband access technologies will play an important role in the National Information Infrastructure. However, the provision of direct fibers to residential and small business customers has always been considered a challenge. To accelerage the provision of Broadband Access Facilities, alternatibe paths could be identified and actaivated in parallel. In this paepr, we present an overview of the alternative access technologies focusing on xDSL, FTTx, HFC, HFR, and suggest the modeling of the access netetwork systems which is using of the technologies. Also to investigate the economics of each access systems, we derve the numerical equations which is summed up the al parameter including the labor cost of the model system. The proposed equation has a variable which is the cell size (or subsciber clusters) and calculate the real cost values which is normalized by the copper cable unit cost. From the numerical resutls, we conclude that the economic access method to provide these broadband capabilities is depended on the subscriber penetration, the patterns of subscriber distribution density and existing communication infrastructures. It can be expected that our analytical model and equations provides an elegant solution to choose the optimal broadband access technologies to residential and small business customers.

무선 LAN 과 무선 ATM LAN

정해원,이영교,조성준 한국통신학회 1998 정보와 통신 Vol.15 No.2

석면에 의한 CHO 세포의 염색체 이상 유발 기전에 관한 연구

정해원,김현주 한국독성학회 1995 Toxicological Research Vol.11 No.1

In order to examine the mechanism of asbestos clastogenicity, CHO cells were treated with chrysotile and crocidolite. Crocidolite and chrysotile were able to induce lipid peroxidation in a dose dependent manner. Ultrafiltrate of culture media from CHO cells treated with chrysotile/crocidolite induced sister chromatid exchange in CHO cells. Ultrafiltrate of culture media from CHO cells treated with chrysotile induced chromosome aberration but it was not statistically significant. Simultaneous treatment of 3-Aminobenzamide (3-AB) or cytosine arabinoside (Ara C) with crocidolite had no effect on the frequency of chromosome aberration by crocidolite whetease posttreatment of caffeine significantly increased the chromosomel aberration by crocidolite. This indicated that DNA damage by asbestos took place at late stage of cell cycle. The results suggested that the ultrafiltrate of media contained clastogenic factor (CF) and lipid peroxidation might be involved in the formation of CF.

광대역 무선 가입자 망의 현황 및 관련 기술 개발 동향

정해원,박봉혁,이형호 한국통신학회 1999 정보와 통신 Vol.16 No.10

방사선에 의한 염색체이상과 DNA 함량과의 관계

정해원,김수영,하성환,김태환,조철구,Chung, Hai-Won,Kim, Su-Young,Ha, Sung-Whan,Kim, Tae-Hwan,Cho, Chul-Koo 대한방사선방어학회 2001 방사선방어학회지 Vol.26 No.2

To study the relationship between the DNA content of human chromosomes and their involvement in radiation-induced structural chromosome aberrations, the frequencies of translocations and dicentrics induced in human lymphocytes after in vitro exposure to 2Gy of x-tay were analysed by fluorescence in situ hybridization(FISH). Single whole chromosome probes(WCP), specific for chromosomes 1, 2, 4, 7, 8, 9, and 21 and triple combination of probes tot chromosome 1, 2 & 4 were used separately. A significant deviation of the frequency of symmetrical translocation and dicentrics from a DNA-proportional distribution was observed. Chromosomes 2, 7, 8, 9 and 21 were less frequently involved in the formation of symmetrical translocations and dicentrics than expected, whereas chromosomes 1 and 4 were more frequently involved. Chromosome 2 and 4 showed a higher frequency of acentric fragments. When triple combination probe for chromosome 1, 2 & 4 was used, no differences were found between the observed and expected frequency of exchange type aberrations. The results showed that the frequency of radiation-induced chromosome aberrations was not proportional to DNA contents, suggesting the difference in the susceptibility to specific aberrations among individual chromosomes. The results also indicated that the FISH technique with combination of probes for chromosome 1, 2 & 4 was useful for radiation biodosimetry.

FISH기법 및 단세포전기영동기법을 이용한 저선량 방사선에 의한 DNA 상해 및 염색체이상 평가

정해원,김수영,김병모,김선진,김태환,조철구,하성환,Chung, Hai-Won,Kim, Su-Young,Kim, Byung-Mo,Kim, Sun-Jin,Kim, Tae-Hwan,Cho, Chul-Koo,Ha, Sung-Whan 대한방사선방어학회 2000 방사선방어학회지 Vol.25 No.4

Comparative study was performed for the assessment of DNA damage and Chromosomal aberration in human lymphocyte exposed to low dose radiation using fluorescence in situ hybridization(FISH) and single cell gel electrophoresis(SCGE). Chromosomal aberrations in human lymphocytes exposed to radiation at doses of 5, 10, 30 and 50cGy were analysed with whole chromosome-specific probes by human chromosome 1, 2 and 4 according to PAINT system. FISH with chromosome-specific probe has been used to be a valid and rapid method fer detection of chromosome rearrangements induced by low dose radiation. The frequencies of stable translocation per cell equivalents were 0.0116, 0.0375, 0.040f, 0.0727 and 0.0814 for 0, 5, 10, 30 and 50cGy, respectively, and those of dicentric were 0.00, 0.0125, 0.174, 0.0291 and 0.0407 respectively. Radiation induced DNA damage in human lymphocyte in a dose-dependent manner at low doses from 5cGy to 50cGy, which were analysed by single tell gel electrophoresis(SCGE). From above results, FISH seemed to be useful for radiation biodosimetry by which the frequencies of stable aberrations in human lymphocyte can be observed more easily than by conventional method and SCGE also seemed to be sensitive method f9r detecting DNA damage by low dose radiation exposure, so that those methods will improve our technique to perform meaningful biodosimetry for radiation at low doses.