해양생물 추출물의 대장암세포주에 대한 항암 작용 검색

정주희 한국식품위생안전성학회 2017 한국식품위생안전성학회지 Vol.32 No.1

Recently, the area of marine resources has become concerned with sources for the next generation of the bio-industry. Until present, development of the marine resources has remained limited, although a large number of these resources are considered to have potential for various significant biological activities. Most marine sponges, marine algae and coral could be used to create specific compounds for survival against a harsh environment. Therefore, it was necessary that these materials needed to be elucidated with biological activities, such as like anti-inflammatory, anti-viral or anti-cancer effects for their utilization in the bio-industry. In this study, we screened extracts of marine resources for their anti-cancer effect on human colorectal cancer cells. These resources were collected at Kosrae of Micronesia on April, 2013 and extracted with methanol. Cytotoxicity of marine resources was observed. Of a total of 20 specimens, three specimens dose-dependently demonstration inhibition of cell viability. Furthermore, cells treated with these specimens for 48h were induced p53, p21, Bax and caspase-3. The results suggest that they involved p53-mediated apoptosis. Two positive specimens (1304KO-327 and 1304KO-329) were verified as the identical materials, which are Hyrtios sp. Unfortunately 1304KO-207 was not yet classified and needed to identify in the further study. There results suggested that marine resources with positive potential in anticancer effect would be good candidates as useful bio-resources.

중앙 집중식 스케줄링 기반 라우팅 프로토콜을 사용한 신뢰성 높은 424MHz IoT 네트워크의 설계 및 구현

정주희,권구인 한국차세대컴퓨팅학회 2018 한국차세대컴퓨팅학회 논문지 Vol.14 No.3

Communication over long distance using low power are the basic requirements for wireless IoT network. Current IoT network routing protocols only handles uplink transmissions. However, to control these IoT devices, downlink data transmissions are required. For the control message transmissions, it should have 100% reliability unlike other data transmission. Moreover, since traffic will be increased by bi-directional transmission, stable data transmission will not be possible with a contention-based MAC layer alone. Therefore, this paper proposes IoT network design using centralized scheduling-based routing protocol. The proposed method sets the path, and sends packets based on the central scheduling to prevent packet collision in advance. Bi-directional reliable links are established by considering parameters such as PER, RSSI and LQI using 424MHz band which is strong against interference and available for long distance communication. Experimental results show that the proposed method transmits data with high reliability. 무선 IoT 네트워크는 낮은 력을 사용하여 장거리에서도 통신이 가능하도록 설계되어야 한다. 이를 한 기존의 라우 로토콜은 상향 링크로의 데이터 송만을 고려하다. 그러나 IoT 기기들을 제어해야 하는 경우에는 하향 링크로의 송 한 필요하며 제어 메시지 송은 일반 데이터 송과 달리 100% 신뢰성을 보장해야 하는데 양방 향 송으로 인해 트래픽이 많아질 경우 경쟁기반의 MAC 로토콜로는 안정인 데이터 송이 불가능하다. 따라 서 본 논문에서는 앙 통제하의 스링 기반 라우 로토콜을 사용한 IoT 네트워크 설계 방법을 제안한다. 제 안한 방법은 앙의 스링 기반으로 경로 설정과 패킷 송을 제어하여 패킷 충돌을 사에 방지한다. 경로 설정 시 양방향 모두 PER, RSSI, LQI 등의 라미터를 고려하고, 간섭에 강하고 장거리 통신이 가능한 424MHz 역을 사용하여 안정인 링크를 설정한다. 시뮬이션이 아닌 필드실험을 하여 PCB 제작된 통신모듈로 실험 을 하으며, 실험 결과 제안한 방법이 매우 안정으로 데이터를 송함을 확인할 수 있었다.

Chalcone-templated Hsp90 inhibitors and their effects on gefitinib resistance in non-small cell lung cancer (NSCLC)

정주희,오용진,권택규,서영호 대한약학회 2017 Archives of Pharmacal Research Vol.40 No.1

The molecular chaperone Hsp90 has emerged asan attractive cancer therapeutic target due to its role incellular homeostasis by modulating the stabilization andmaturation of many oncogenic proteins. In this study, wedesigned and synthesized a series of Hsp90 inhibitors thathybridized NVP-AUY992 (2) and PU3 (3) in the chalconescaffold using a structure-based approach. Our resultsindicate that compound 1g inhibited the proliferation ofgefitinib-resistant non-small cell lung cancer (H1975) cells,downregulated the expression of client proteins of Hsp90including EGFR, MET, Her2 and Akt, and up-regulated theexpression of Hsp70. The compound 1g represents a newclass of Hsp90 inhibitors with a chalcone structure. Thedesign, synthesis, and evaluation of 1g are describedherein.

COVID-19 유행시기에 병원간호사의 외상후스트레스와 정서적소진의 관계에서 회복탄력성의 매개효과

정주희,송영숙 한국간호교육학회 2022 한국간호교육학회지 Vol.28 No.2

Purpose: This study explored the mediating role of resilience between post-traumatic stress and emotional exhaustion in nurses during the COVID-19 pandemic. Methods: The data were collected from 193 nurses working at a hospital in Daegu from October 16 to 30, 2020, in the middle of the COVID-19 pandemic. Participants conducted self-reported surveys of post-traumatic stress (Impact of Event Scale-Revised Korean version), emotional exhaustion (Maslach Burnout Inventory), and resilience (Korean Resilience Quotient Test). The data were analyzed using descriptive statistics, frequency, t-tests, Mann-Whitney U tests, Kruskal-Wallis tests, Pearson correlation coefficients, and multiple regression. Mediation analysis was performed using the Baron and Kenny method and Sobel test. Results: The mean scores for post-traumatic stress, resilience, and emotional exhaustion were 16.18±16.18, 183.41±18.29, and 32.64±9.66, respectively. A higher level of post-traumatic stress was associated with lower resilience (r=-.20, p=.004) and with higher emotional exhaustion (r=.33, p<.001). A higher level of resilience was associated with lower emotional exhaustion (r=-.35, p<.001). The resilience was confirmed to have a partial mediating effect in the relationship between post-traumatic stress and emotional exhaustion, verified by the Sobel test (Z=2.31, p=.02). Conclusion: Resilience had a partial mediating effect in the relationship between post-traumatic stress and emotional exhaustion. Thus, nurses should develop individual strengths to reduce emotional exhaustion, and managers should also make efforts to increase the nurses’ resilience. It is also necessary to develop and apply a program to strengthen nurses’ resilience.

중년층 맞벌이 여성의 HMR 선택속성이 충성도에 미치는 영향: 기업 신뢰에 대한 매개역할을 중심으로

정주희 (사)한국조리학회 2019 한국조리학회지 Vol.25 No.11

The purpose of this study was to examine the effects of HMR selective attributes on loyalty and to examine whether corporate trust plays a role between HMR selective attributes and loyalty among middle-aged working women consumers. The survey was conducted from March 12, 2019 to April 27, 2019 by the convenience sampling method. A total of 300 questionnaires were distributed and the final 236 copies were used as useful data. The analysis results are as follows. First, the practicality among the HMR selective attributes of middle-aged working women showed the most significant effect on loyalty, and the material safety had a negative effect on loyalty. Second, corporate trust plays an mediating role between practicality and loyalty among HMR selective attributes of middle-aged working women. Therefore, this study contribute to provide useful data for securing loyal customers by grasping the middle-aged women's selective attributes for HMR products. In addition, present study has helped to build a positive corporate image through corporate trust.

공정무역 커피에 대한 소비가치가 브랜드 태도와 구매의도에 미치는 영향

정주희,최미선,김예영,Jung, Ju Hee,Choi, Mi Sun,Kim, Ye Young 한국식생활문화학회 2017 한국식생활문화학회지 Vol.32 No.5

When purchasing products or services, consumers tend to purchase multiple value attributes through the acquisition of a product rather than physical characteristics. This study empirically analyzed the relations between consumption value, brand attitude, and purchase intention of customers purchasing fair-trade coffee, by dividing consumption values that would have significant effects on consumers' actual purchase behavior into five levels. Among the consumption value factors of customers purchasing fair-trade coffee, the functional value, social value, emotional value, and rare value all had significant effects on brand attitude and purchase intention while the influence of situational value on brand attitude and purchase intention was not verified. These finding are partially concordant with those of preceding studies, and the consumption value of consumers purchasing fair-trade coffee has positive effects on purchase behavior; therefore, it would be necessary for companies selling fair-trade products to understand consumers' specific values. Moreover, analysis of the influence of brand attitude on purchase intention of customers purchasing fair-trade coffee revealed that purchase intention increased when brand attitude was higher. These finding indicate that it is necessary to identify measures to increase purchase intention by targeting consumer groups with high brand attitude with intensive promotions.

식용곤충식품에 대한 소비자 인식 연구

정주희,임배균,배세정 한국식생활문화학회 2018 한국식생활문화학회지 Vol.33 No.6

The purpose of this study is to investigate consumers’ perception of edible insect foods and carry out importance-satisfaction research on the choice attributes of edible insect foods. Through that, this researcher intends to examine the potential of food materials as future food resources and provide foundational data to devise marketing strategies needed to develop products made of edible insect foods. According to the results, female have more information about edible insect foods than male (p<0.05), and male gained higher points in challenging for new foods (p<0.001), overall satisfaction (p<0.001), and purchase intention (p<0.01) than female. Both importance (p<0.05) and satisfaction (p<0.001) about the mean of the choice attributes of edible insects are found to be higher in male than female. The sustenance area showing high importance and high satisfaction both includes taste, smell, freshness, sanitation, safety, and shelf-life. Accordingly, to understand edible insect foods righteously, it is needed to deliver information constantly and publicize them aggressively. Also, it is necessary to develop various kinds of food made of edible insect foods and functional food utilizing their nutritive value.

컴퓨터를 이용한 수리논리학 교육

정주희 대한수학교육학회 1999 수학교육학연구 Vol.9 No.1

This paper discusses jLogic, a mathematical logic education software developed by the author. jLogic is basically a MS-Windows based software that can construct first-order models, formulas and thet their satisfiablity. Logical formulas are easily input by a "keyboard" maintained by jLogic. A special finite model, called the "Toy World" can be visually cinstructed and modified. The user is supposed to answer the following 3 questions about the selected logical expression: 1. Is it a grammatically correct logical formula? 2. Is it a sentence that has a definite truth value? 3. Is th sentence true or false? When the user inputs his answer in the "Inspector window" and then presses the OK button, jLogic instantly tests the validity of the answer and tells the user the result. jLogic is freely downloaded from http://gauss.kyungpook.ac.kr/~jlogic/

초임계 이산화탄소를 이용한 5-FU 함유 생분해성 고분자 미세입자 제조

정주희,정인일,주현재,신재란,임교빈,유종훈 한국생물공학회 2008 KSBB Journal Vol.23 No.5

To obtain maximal efficacy with minimal systemic side-effects, many studies have been carried out to achieve the controlled release of 5-fluorouracil (5-FU). In this study, biodegradable poly(L-lactide) (L-PLA) microparticles containing 5-FU were prepared by a process, called aerosol solvent extraction system (ASES), utilizing supercritical carbon dioxide. The effects of various organic solvents, drug/polymer feeding ratio, polymer molecular weight, and blending with the same polymers with different molecular weights on the formation of 5-FU loaded microparticles were investigated under a predetermined operating condition from our previous study. The drug recovery, entrapment efficiency, and in vitro drug release kinetics were determined by HPLC assays. The drug recovery obtained from the ASES process was found to be very high, whereas the drug entrapment efficiency was considerably low in all the experiments due to the poor affinity between L-PLA and 5-FU. These results indicated that the precipitation rate of L-PLA might be quite different from that of 5-FU so that there was little chance to form 5-FU loaded L-PLA microparticles.

뷰티 유튜버 채널 구독동기가 추천의도에 미치는 영향: 만족도의 매개효과를 중심으로

정주희,홍원기 한국무역연구원 2024 무역연구 Vol.20 No.5

Purpose – Recently, video platforms like YouTube have become primary channels for consumers to gather information and form opinions about products. This powerful medium surpasses the impact of traditional media in corporate marketing. In this environment, Beauty YouTubers have emerged as influential figures that significantly affect consumer choices and purchasing decisions. Based on this background, this study aims to analyze the mediating effect of viewer satisfaction on the relationship between subscription motivation for beauty YouTuber channels and consumer intention to recommend. Viewer satisfaction is considered a crucial variable that mediates the communication process between information providers and consumers in today's video-centric environment. Design/Methodology/Approach – An online survey using Google Forms was conducted, and data from 202 consumers subscribing to Beauty YouTuber channels were used for analysis. Findings – The research hypotheses were tested using SPSS Process Macro, and the results showed that all subscription motivations, playfulness, professionalism, and recency, significantly influence the intention to recommend. Additionally, viewer satisfaction was found to partially mediate the relationship between subscription motivation and recommendation intention. Research Implications – These findings suggest that companies collaborating with Beauty YouTubers should recognize the importance of subscription motivation and viewer satisfaction to enhance recommendation intention.