국민의 식생활과 건강보조식품의 필요성에 관한 연구

이정수 대구산업정보대학 1995 논문집 Vol.9 No.2

As mentioned above,we know that the intake of food by people in our country has nearly approched the encouraged amount of nutrition. Based on this fact, many nutritionists conclude that there is no health impediment due to malnutrition, for the nutritive condition of Korean people today is much better than that in the past,and the intake of food and the encouraged amount of nutrition are nearly balanced. Nevertheless, we should not overlook a few points:imperfection of the encouraged amount of nutrition itself;probable imbalance of nutrition due to excessive intake of manufactured food and fast food;the menace to human health due to theaggravation of environmental pollution ; and probable malnutrition due to the destruction of nutrients during the process of cooking because of imperfect education for nutrition. Therefore, in order to maintain the necessary balance of perfact nutrition, we should duly consider the following points : 1. Elimination of environmental pollution. 2. Consumption of food and drink with natural nutrition(without the process of manufacture and cooking) if possible. 3. Reduction of the intake of manufactureed food as much as possible. 4. Consumption of high fiber food and low carbohydrate food if possible. Putting aside various points of view,we can see that the condition of human health is always threatened by various factor even though the dietary life for human health has nearly approached the encouraged amount of nutrition. Therefore, we should empasize the necessity of preventive measure or improvement of supplemental food for human health.

Verapamil이 심장의 교감신경 β-수용체에 미치는 영향

이정수 ( Jeung Soo Lee ),은종영 ( Jong Young Eun ),조명제 ( Myung Jae Cho ) 한국위생과학회 2004 한국위생과학회지 Vol.10 No.1

개와 쥐 심실의 Muscarinic receptor의 성질에 관한 연구

이정수 ( Jeung Soo Lee ),김명주 ( Myung Joo Kim ) 한국위생과학회 2003 한국위생과학회지 Vol.9 No.1

인삼 Saponin, 은시호 Saponin 및 계면활성제가 적혈구의 용적변화 및 Fragility에 미치는 영향

이신웅(Shin Woong Lee),이정수(Jeung Soo Lee),이수근(Soo Kun Lee),옥창건(Chang Kun Ok),김영희(Young Hie Kim) 대한약학회 1989 약학회지 Vol.33 No.1

The effects of Gypsophila saponin, sodium dodecylsulfate (SDS) and Triton X-100 on volume changes and fragility of red blood cells were compared to ginseng saponin to elucidate whether there are any difference in their action on membrane lipid. Cell volume was decreased to about 38% in 1M NaCl and increased to about 20% in 1/10M NaCl. Hematocrit value was decreased by Gypsophila saponin, SDS, and Triton X-100 which caused hemolysis in isotonic NaCl solution. These detergents also inhibited increase of cell volume and accelerated hemolysis in hypotonic solution. However, ginseng saponin did not influence to osmotic volume changes and hemolysis of red blood cells. These results suggest that the disruptive effect of plant saponin on membrane barriers induced by removing membrane lipid is different from their source and ginseng saponin has very low affinity to membrane lipid.

쥐 뇌 Na+ , K+ -ATPase 의 활성 및 [3H] Ouabain 결합에 미치는 Cadmium 의 영향

이신웅(Shin Woong Lee),이정수(Jeung Soo Lee) 영남대학교 자원문제연구소 1991 자원문제연구논문집 Vol.10 No.-

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인삼 사포닌이 개 심실 형질막의 K+-의존성 포스파타제 활성에 미치는 영향

이신웅(Shin Woong Lee),이정수(Jeung Soo Lee) 대한약학회 1992 약학회지 Vol.36 No.2

The effects of ginseng saponins, gypsophila saponin, sodium dodecyl sulfate(SDS), and Triton X-100 on membrane K+-dependent phosphatase activity which is lipid dependent and represents dephosphorylation step of the complete Na+, k+-ATPases reaction were investigated in this study to elucidate whether the effects of ginseng saponins are due to the detergent action, using sarcolemma enriched preparation isolated from dog ventricle. Na+, K+-ATPase and K+-dependent phosphatase activities of cardiac sarcolemma wee about 143mcmol Pi/mg protein/hr and 34mcmol p-nitrophenol/mg protein/hr, respectively. While ginseng saponins(triol>total>diol) inhibited K+-dependent phosphatase activity, gypsophila saponin, and low dose of SDS(0.4mcg/mcg protein), and Triton X-100 (0.6mcg/mcg protein) increased the enzyme activity, indicating disruptive effect of detergents on membrane barriers. The activating effect of low doses of Triton X-100 on membrane K+-dependent phosphatase appeared at concentration decreasing light scattering. However, the inhibitory effect of ginseng saponin apperared before a decrease in light scattering. Theses results suggest that low concentrations of ginseng saponins inhibit the membrane K+-dependent phosphatase by interacting directly with enzyme before membrane disruption.

자궁 무스카린수용체의 확인 및 분류

이신웅(Shin Woong Lee),이정수(Jeung Soo Lee),박영주(Young Joo Park) 대한약학회 1992 약학회지 Vol.36 No.3

The muscarinic acetylcholine receptors of the dog unpregnant uterus were characterized using [3H] quinuclidinyl benzilate(QNB) as a radioligand and the binding of muscarinic receptor agonists and antagonists in the uterus was compared to that in the urinary bladder which contains almost exclusively the M2 receptors in order to determine the receptor subtypes in the uterus. [3H]QNB binding to uterus and bladder was rapid, saturable and reversible. Scatchard analysis of the saturation data gave linear plots and the Hill coefficients were close to unit, which indicated that each preparation contained a single population of specific binding sites for [3H]QNB. The KD values(l2OpM) for QNB were almost identical in both organs, whereas the Bmax value of 256fmol/mg protein in the uterus was significantly different from that of 563fmol/mg protein in the bladder. Muscarinic agonists and antagonists inhibited in a competitive manner the [3H]QNB binding to the same extent in both organs. The competition binding studies using antagonists(atropine and pirenzepine) exhibited a single binding site and this site had a low affinity for pirenzepine with the Ki value of about 330nM. However, high and low affinity binding sites were observed with carbachol, methacholine and oxotremorine. These binding studies with agonists and antagonists did not show any differences in drug affinities between uterus and bladder. These results indicate that the muscarinic receptors in the uterus are M2 receptors which have a low affinity for pirenzepine.

칡 열수추출물이 흰쥐의 알코올 대사효소계에 미치는 영향

김명주(Myung-Joo Kim),이정수(Jeung-Soo Lee),하오명(Oh-Myung Ha),장주연(Joo-Yeun Jang),조수열(Soo-Yeul Cho) 한국식품영양과학회 2002 한국식품영양과학회지 Vol.31 No.1

갈화와 갈근이 알코올성 간손상 흰쥐의 알코올 대사효소계에 미치는 영향을 구명하기 위하여 알코올을 투여한 흰쥐에게 갈화와 갈근 열수추출물을 2수준으로 나누어 5주간 급여한 후 그 효과를 관찰하였다. 체중증가량, 식이섭취량과 식이효율은 알코올 투여시 감소하였으며 갈화와 갈근추출물 급여로 회복되는 경향을 보였다. 장기 무게에 미치는 칡추출물의 영향은 알코올 투여로 증가된 간, 뇌와 심장 무게를 감소시켰는데 갈화가 갈근에 비하여 뇌와 심장 무게의 감소효과가 큰 것으로 나타났다. 알코올 대사효소 활성 변화에서 알코올 투여시 유의적으로 증가된 ADH와 MEOS 활성은 칡추출물 급여시 감소된 반면 AlDH 활성은 대조군에서 유의적으로 감소되었는데 칡추출물 급여시 그 활성이 증가되었다. MAO 활성과 과산화수소 함량도 알코올 투여시 증가되었으며 갈화와 갈근 열수추출물 급여시 유의적으로 감소되었다. 이상의 결과에서 갈화와 갈근 열수추출물 급여는 알코올 중독된 흰쥐의 알코올 대사계 효소 활성 변화에 영향을 미쳐 알코올성 간손상 완화에 효과적일 것으로 사료된다. The effects of Pueraria flos (PF) and Pueraria radix (PR) water extract on the hepatic alcohol metabolic enzyme activities were examined in rats that were orally administered ethanol (25% v/v, 5 g/kg body weight/day) for 5 weeks. The PF and PR water extract were supplemented in a diet, based on 1.2 g or 2.4 g of raw PF or PR/kg body weight/day. Alcohol administration without the PF or PR supplementation significantly decreased net weight gain, feed intake and feed efficiency ratio. However, both dose of the PF or PR supplementation resulted in significant enhancement of growth and suppression of increased relative weight of liver, brain and heart by alcohol administration. Activities of hepatic alcohol dehydrogenase and microsomal ethanol oxidizing system were higher in the alcohol treated group than in the normal group, while aldehyde dehydrogenase activity was significantly lowered in the alcohol treated group. The hepatic metabolic enzyme activities altered by alcohol administration were normalized by both doses of PF or PR supplement. Hepatic monoamine oxidase activity and hydrogen peroxide, which were significantly higher in the alcohol treated group than in the normal group, were also decreased by the supplementation with either PF or PR. These results indicate that low- or high- supplementation of either water extract PF or PR may alleviate ethanol-induced hepatotoxicity by altering alcohol metabolic enzyme acitivities.

항 Histamine제와 Muscarinic Receptor와의 상호작용(II) -대뇌 Muscarinic M1 Receptor에 대한 작용-

이신웅(Shin Woong Lee),박영주(Young Joo Park),박인숙(In Sook Park),이정수(Jeung Soo Lee) 대한약학회 1990 약학회지 Vol.34 No.4

A single uniform population of specific, saturable, high affinity binding site of [3H] guinuclidinyl benzilate(QNB) was identified in the rat cerebral microsomes. The Kd value(37.2pM) for [3H]QNB calculated from the kinetically derived rate constants was in agreement with the Kd value(48.9pM) determined by analysis of saturation isotherms at various receptor concentrations. Dimenhydrinate(DMH), histamine H1-blocker, increased Kd value for [3H] QNB without affecting the binding site concentrations and this effect resulted from the ability of DMH to Slow [3H]QNB-receptor association. Pirenzepine inhibition curve of [3H]QNB binding was shallow(nH = 0.52) indicating the presence of two receptor subtypes with high (M1-site) and low(M2-site) affinity for pirenzepine. Analysis of these inhibition curves yielded that 68% of the total receptor populations were of the M1-subtype and the remaining 32% of the M2subtype. Ki values for the M1- and M2-subtypes were 2.42nM and 629.3nM, respectively. Ki values for H1-blockers that inhibited [3H]QNB binding varied with a wide range (0.02-2.5mcM). The Pseudo-Hill coefficients for inhibition of [3H]QNB binding by most of H1-blockers examined except for oxomemazine inhibition of [3H]QNB binding were close to one. The inhibition curve for oxomemazine in competition with [3H]QNB was shallow(nH = 0.74) indicating the presence of two receptor populations with different affinities for this drug. The proportion of high and low affinity was 33:67. The Ki values for oxomemazine were 0.045 +/- 0.016mcM for high affinity and 1.145+/-0.232mcM for low affinity sites. These data indicate that muscarinic receptor blocking potency of H1-blockers varies widely between different drugs and that most of H1-blockers examined are nonselective antagonist for the muscarinic receptor subtypes, whereas oxomemazine might be capable of distinguishing between subclasses of muscarinic receptor.

쥐 심실에서 Digitalis Receptor Desensitization 에 관한 연구

이신웅(Shin Woong Lee),이정수(Jeung Soo Lee),장태수(Tae Soo Jang) 한국응용약물학회 1994 Biomolecules & Therapeutics(구 응용약물학회지) Vol.2 No.2

[³H]Ouabain binding parameters (K_D and B_(max)) to control rat ventricular strips and Langendorff preparations which were not previously exposed to ouabain were compared with those to both preparations that had been first exposed to a complete ouabain dose range of dose-response curve (10^(-8) M to 10^(-4) M). In rat ventricular strips and Langendorff perfused heart preparations, cumulative dose-response curves of ouabain revealed biphasic positive inotropic effects, a low-dose effect and a high-dose effect with ED_(50) values of 0.5μM and 35μM ouabain, respectively. The low-dose effect in ventricular strip disappeared or was diminished significantly when the ouabain dose-response curve was repeated after the washout of the effects of the first dose-response curve, whereas there were no significant differences in the maximal high-dose effect in both exposures to oubain. However, both of the control and ouabain-preexposed Langendorff perfused hearts revealed the same low-dose effects. The K_D value for [³H]ouabain binding and the ouabain binding site concentration (B_(max)) estimated by [³H]ouabain displacement assay in control preparations were 230 nM and 2 pmol/mg protein, respectively. [³H]Ouabain binding parameters were not changed by repeated exposure to high concentrations of ouabain. These results suggest that digitalis receptor desensitization in the rat ventricular strip may due to the change of post-receptor events induced by ouabain binding to a high affinity site (α₂ isoform).