전통주 주박의 항혈전 활성 평가

김미선(Mi-Sun Kim),이예슬(Ye-Seul Lee),김종식(Jong Sik Kim),신우창(Woo-Chang Shin),손호용(Ho-Yong Sohn) 한국생명과학회 2014 생명과학회지 Vol.24 No.8

전통주 주박을 이용한 고부가가치 식품소재 개발을 위해, 상업적 시설에서 생산된 3종 약주(J-B, J-S, J-Y) 및 2종 탁주(J-H, J-W) 주박의 ethanol 추출물 및 열수 추출물을 조제하고 이들의 혈액응고 저해활성, 혈소판 응집저해 활성 및 인간 적혈구 용혈활성을 평가하였다. 5종 주박의 pH는 3.90~4.29로 유사하였으나, brix는 5.0~27.0으로 다양하게 나타났으며, 수분 및 알코올 함량에서도 시료에 따라 1.8배의 차이를 나타내었다. 주박의 색차와 성분은 첨가된 부재료 및 사용누룩에 좌우되었으며, J-W 주박의 경우 수분함량이 80.3%, brix 13, 알코올 함량 1.8%를 함유하여 다른 주박에 비해 다양한 식품제조에 용이하게 이용 가능하리라 판단되었다. Ethanol 추출효율은 J-H, J-W, J-B, J-S, J-Y의 순, 열수 추출효율은 J-S, J-B, J-W, J-H, J-Y의 순으로 높았으며, 총폴리페놀 및 총플라보노이드함량은 ethanol 추출물 중에서는 J-H, 열수 추출물 중에서는 J-Y 주박에서 가장 높았다. 5종 주박의 10종 추출물은 모두 5 mg/ml 농도까지 인간 적혈구에 대한 용혈활성이 나타나지 않았으며, J-B, J-S, J-Y의 약주 주박의 ethanol 추출물에서 유의적인 혈액응고저해 활성이 나타났으며, J-W 탁주 주박의 열수 추출물에서 thrombin 저해 활성과 J-B, J-S 및 J-H 주박 열수 추출물에서 혈액 응고인자 저해활성을 확인하였다. 혈소판 응집저해 활성평가의 경우 J-W 탁주 주박의 ethanol 및 열수 추출물에서만 아스피린에 필적하는 우수한 활성이 확인되었다. 본 연구결과는 다양한 약주 및 탁주 주박이 항혈전 활성을 가지고 있으며, 주박으로부터 항혈전제 개발이 가능함을 제시하고 있다. In this study, ethanol and hot water extracts of lees from Korean traditional wine (J-B, J-S, J-Y, J-H, and J-W) were prepared, and their effects on blood coagulation, platelet aggregation, and hemolysis of human red blood cells (hRBCs) were investigated to develop functional food ingredients from lees. The pH and brix of the lees ranged from 3.90 to 4.29 and 5.0 to 27.0o, respectively, and there was a huge difference in the water and ethanol content among the lees. The nuruk and additives used affected the color and physicochemical properties of lees. The J-W takju made from only rice and traditional nuruk, which has 13o brix and 1.8% of alcohol, has potential as functional food ingredient. With regard to the extraction yields of lees, higher yields were obtained from J-H, which contains different medicinal plants, in ethanol, followed by J-W, J-B, J-S, and J-Y. Higher extraction yields of lees were obtained from J-S in hot water, followed by J-B, J-W, J-H, and J-Y, respectively. The ethanol extract of J-H and the hot water extract of J-Y had the highest contents of total polyphenol and total flavonoids among the lees extracts. The 10 lees extracts did not show hemolysis activity against hRBCs up to 5 mg/ml. In an anticoagulation activity assay, the ethanol extracts of three yakju lees (J-B, J-S, and J-Y) and the hot water extract of J-W inhibited thrombin activity, whereas the hot water extract of J-B, J-S, and J-H inhibited blood coagulation factors. In an antiplatelet aggregation activity assay, only the J-W takju lees showed significant inhibition activity. Our results suggest that lees from traditional wine had high potential as a novel antithrombosis agent.

포스트휴먼 감수성 함양 교육을 위한 미술작품 사례분석

이예슬,허윤정,Lee, Yea-Seul,Huh, Yoon-Jung 중소기업융합학회 2020 융합정보논문지 Vol.10 No.11

The emergence of post-human beings in the 4th Industrial Revolution era led to an epistemological shift in the need for reflection on new relationships with non-human beings, away from human-centered modern humanism. For this reflection, post-human sensibility to empathize and understand the surrounding world is required. In order to cultivate this sensibility, we analyzed the case of art works that can think about and experience the post-human era based on the criteria of 'animal-becoming', 'earth-becoming', and 'machine-being' presented by post-humanist researcher Bridotti. Since the work of art reflects the spirit of the era, we confirmed the positive aspect of the text that can reflect and experience the post-human era. This study is meaningful as a basic study by presenting art works that can be used in art education to improve post-human sensitivity.

고구마 소주 주박의 항균 및 항혈전 활성

김미선 ( Mi Sun Kim ),이예슬 ( Ye Seul Lee ),김종식 ( Jong Sik Kim ),신우창 ( Woo Chang Shin ),손호용 ( Ho Yong Sohn ) 한국미생물생명공학회 2014 한국미생물·생명공학회지 Vol.42 No.3

Sweet potato soju (SPS), a form of traditional distilled alcoholic liquor in Korea, is manufactured by the distillation of fermented broth under normal pressure, thus providing it for a uniquely smooth taste infused with the flavor of sweet potato. After distillation, the lees of SPS is produced as by-product and discarded. In this study, the ethanol and hot water extracts of lees of SPS, and their subsequent organic solvent fractions using hexane, ethylacetate (EA), butanol, and water residue were prepared in an effort at the efficient re-use of the lees of SPS. The ethanol extraction yield was 1.36-fold higher than that of the hot water extraction, and the EA fraction revealed the highest total polyphenol content among the solvent fractions. The various extracts and solvent fractions did not demonstrate hemolytic activity at up to 0.5 mg/ml concentrations against human red blood cells. In the bio-activity assay, only the EA fraction displayed a broad spectrum of anti-microbial activity against different pathogenic and food spoilage bacteria, and demonstrated significant anti-coagulation activity by inhibitions of thrombin, prothrombin and blood coagulation factors. Furthermore, only the EA fraction from the hot water extract of the lees of SPS showed anti-platelet aggregation activity, which is comparable to aspirin (a commercially available drug). Our results suggest that the EA fraction of the hot water extract prepared from the lees of SPS has a high potential as a novel resource for anti-microbial and anti-thrombosis agents.

물리적 착유 국산 대두박 활용 추출 단백질 특성 분석

이예슬 ( Ye-seul Lee ),정현경 ( Hyun-kyung Jung ),이주오 ( Ju-o Lee ),유현채 ( Hyeon-chae Yoo ),이현동 ( Hyun-dong Lee ) 한국농업기계학회 2025 한국농업기계학회 학술발표논문집 Vol.30 No.2

인동덩굴 추출물과 분획물의 항산화 활성 및 산화적 스트레스에 대한 세포 보호 효과

이예슬 ( Ye Seul Lee ),윤믿음 ( Mid Eum Yun ),이윤주 ( Yun Ju Lee ),박영민 ( Young Min Park ),이상래 ( Sang Lae Lee ),박수남 ( Soo Nam Park ) 한국미생물생명공학회 2018 한국미생물·생명공학회지 Vol.46 No.1

In this study, the antioxidant activities and cytoprotective effects against oxidative stress of Lonicera japonica Thunb. 50% ethanol extract and ethyl acetate fraction were investigated. Using the 1,1-diphenyl- 2-picrylhydrazyl assay, the free radical scavenging activity (FSC50) of L. japonica Thunb. 50% ethanol extract and ethyl acetate fraction was determined as 152.00 and 77.25 μg/ml, respectively. To measure the reactive oxygen species (ROS) scavenging activity, the total antioxidant capacity (OSC50) was determined by using a luminol-dependent chemiluminescence assay. The antioxidant activity of the ethyl acetate fraction (0.33 μg/ml) was approximately four times stronger than that of the 50% ethanol extract (1.12 μg/ml). The protective effect against ¹O₂-induced cellular damage of human erythrocytes (τ₅₀) was 46.0 min at 10 μg/ml of the 50% ethanol extract and 52.3 min at 1 μg/ml of the ethyl acetate fraction. We also investigated the cytoprotective effects against oxidative stress induced by H₂O₂ and the intracellular ROS scavenging activity in response to UVB irradiation and found that the extract and fraction protected human skin cells from damage and reduced ROS. These results confirmed that L. japonica Thunb. was a valuable plant-derived natural antioxidant with potential for development as an antioxidative functional ingredient.

흰점박이꽃무지 수확 후 세척-데침-건조공정에 따른 품질분석

이예슬 ( Ye-seul Lee ),박경민 ( Kyung-min Park ),정현경 ( Hyun-kyung Jung ),이주오 ( Ju-o Lee ),유현채 ( Hyeon-chae Yoo ),이현동 ( Hyun-dong Lee ) 한국농업기계학회 2025 한국농업기계학회 학술발표논문집 Vol.30 No.2

국가습지 DB를 활용한 남한 내륙습지의 토지피복 유형 특성 분석

이예슬 ( Lee Ye-seul ),윤혜연 ( Yoon Hye-yeon ),이성호 ( Lee Seong-ho ),장동호 ( Jang Dong-ho ),윤광성 ( Yun Kwang-sung ),이창수 ( Lee Chang-su ) 한국지형학회 2020 한국지형학회지 Vol.27 No.4

This study modified the properties and boundaries of the inland wetland types through the structural edit of the National Wetland DB, and analyzed the characteristics of the different land cover by area and the entire inland wetlands of South Korea. The inland wetlands of the Gangwon Basin had a small area of waters. In addition, the ratio of natural barren was high, reflecting the characteristics of the upper reaches of the large river in the east and west part of Gangwon Province. The Geum River Basin had a high percentage of aggregate land due to the development of large alluvial land, and the ratio of artistic barren was low, so various ecosystem service of wetland elements were distributed evenly. The Nakdong River Basin had a high proportion of waters as water level in the channel rose due to the installation of 4 Major Rivers Beam, and the ratio of Natural barren was low. Moreover, the water level of the main attributes flowing into the Nakdong River drainage system was not high, so the ratio of vegetation concentration was high. The Yeongsan River Basin showed that Waters had the high proportion. And the distribution of Natural barrens represented differently according to the Yeongsan River Basin and the Seomjin River Basin. Finally, Sand and Gravels supplied to rivers during precipitation were deposited in the main stream of the Han River Basin, and the differences between the side and high side was large in the area, reflecting the characteristics of the mouth of a river, so the Natural barren of Clay was distributed.

생팥과 증자팥의 성분 및 생리활성 비교

이륜경 ( Ryun Kyung Lee ),김미선 ( Mi Sun Kim ),이예슬 ( Ye Seul Lee ),이만효 ( Man Hyo Lee ),이종화 ( Jong Hwa Lee ),손호용 ( Ho Yong Sohn ) 한국미생물생명공학회 2014 한국미생물·생명공학회지 Vol.42 No.2

In the course of study for the development of functional food using red beans (azuki beans, Phaseolus radiatus L.), the ethanol extracts from raw-red bean (RRB) and boiled-red bean (BRB) were prepared, and the components and various biological activities of both were compared. It was observed that the extraction yield, and the total polyphenol content, of the BRB were 1.2 times higher than that of the RRB. However, the contents of total flavonoid, total sugar and reducing sugar in the BRB were 30, 27.9 and 30.8% respectively when compared with those of RRB. In relation to antioxidative activity, both RRB and BRB exhibited moderate DPPH anion, ABTS cation, and nitrite scavenging activities and reducing power, though in all cases RRB demonstrated stronger activities than BRB. The extracts of RRB and BRB did not reveal any antimicrobial activities. In a α- amylase inhibitory activity assay, RRB was higher than BRB, while BRB showed higher α-glucosidase inhibitory activity than RRB. A strong and particular activity was observed in an anti-thrombosis activity assay of RRB. The extract of RRB demonstrated strong inhibitions against prothrombin and blood coagulation factors, with moderate thrombin inhibition. However, the extract of BRB did not exhibit any significant anti-thrombosis activity. Our results indicate that RRB has different, but useful biological activities, and loss or elimination of the biologically active substances in RRB occurs during the production of BRB. Therefore, to develop more functional foods from red beans, a study of suitable boiling, heating and drying processes is essential, and the efficient re-use of boiled waste water from the boiling process is necessary. These results could be applied to the further development of functional red bean beverages and sweat red bean pastes.

무 · 배추 수집기 개발을 위한 최근 연구동향 분석

이예슬 ( Ye-seul Lee ),김종문 ( Jong-mun Kim ),김용주 ( Yong-joo Kim ),정다운 ( Da-un Jeong ),강태경 ( Tae-kyung Kang ),이상희 ( Sang-hee Lee ),정선옥 ( Sun-ok Chung ) 한국농업기계학회 2017 한국농업기계학회 학술발표논문집 Vol.22 No.2

밭작물 기계화를 위해서는 단일 기계뿐 아니라 생산 전과정기계화 작업체계가 완성되어야 한다. 특히 무· 배추는 김치의 주재료이며 수확작업의 대부분이 인력으로 수행되고 있어 기계화 기술 개발이 시급하다. 또한 자주식 개발 연구가 일부 진행 중이지만, 기계화율을 향상시키고 농업기계 임대사업 등의 효과를 극대화하기 위하여 트랙터 부착형 무·배추 수집기 개발이 필요하다. 따라서 본 연구에서는 트랙터 부착형 무·배추 수집기 개발을 위한 연구동향을 분석하였다. 국외 제품 분석 결과, 주로 보행형, 트랙터 부착형 또는 트랙터-트레일러와 연계하여 후처리까지 수행하는 수확기의 개발이 진행되었다. 뿌리절단날로는 진동굴취날, 원판뿌리절단날, 원판뿌리유도날 등이 사용되고 있으며 이송부의 컨베이어벨트로는 연질고무돌기부착벨트와 평벨트를 주로 사용하고 있다. 일본 Yanmar사 26마력 엔진 무 수확기는 크게 주행부, 수확부, 수취부로 나눠져 있다. 변속방식은 HST 무단변속, 주행방식은 무한궤도식이다. 덴마크 ASA-Lift사 양배추 수확기는 1조식, 2조식 방식으로 접이식 적재함을 탑재하고 있는 트랙터 부착형 배추수확기이다. 국내 연구 동향 분석 결과, 1998~2001년에 농촌진흥청에서 수행된 “배추재배 일관기계화 시스템”연구의 세부과제로 개발된 “트랙터 부착형 배추 수확기”가 유일한 연구이며, 배추물성 측정, 주요 설계요인에 대한 기초시험, 시작품에 대한 현장실증시험 등 체계적인 연구를 수행하였다.

마늘 생산 전과정기계화 현장적응 시험

이예슬 ( Yeseul Lee ),최일수 ( Ilsu Choi ),강나래 ( Narae Kang ),김영화 ( Younghwa Kim ),우제근 ( Jeakeun Woo ),김영근 ( Youngkeun Kim ),최용 ( Yong Choi ),권영석 ( Youngseok Kwon ),김대철 ( Daecheol Kim ) 한국농업기계학회 2020 한국농업기계학회 학술발표논문집 Vol.25 No.1

마늘 재배 기계화율은 ’18년 58.6%로 보고되고 있으며, 파종(15.9%)과 수확(36.7%) 작업의 기계화율이 미흡한 실정이다. 또한, 최근 5년간 마늘의 총 생산비용 중 노동비가 52.23%를 차지하고 있어 노동력과 생산비 절감을 위해 마늘 재배 전과정에 농기계를 투입한 전과정기계화 시스템개발이 필요하다. 따라서 본 연구에서는 마늘 재배 생력화를 위해 경운·정지에서 수확까지 마늘 생산 주요 작업공정에 농기계를 투입하여 전과정기계화 작업체계 확립 및 효과분석을 수행하고자 하였다. 현장적응 시험은 난지형 마늘을 대상으로 수행하였으며, 전라남도 무안군 바이오에너지작물센터 구내 포장과 충청남도 공주시 우성면 소재 농가포장에 각각 시험포장을 조성하였다. 최근 난지형 마늘 수확작업의 경우 기존의 ‘굴취→비닐제거→수집→줄기절단’에서 노동력 부족에 따라 ‘줄기절단→비닐제거→굴취→수집’체계로 바뀌는 경향이 조사되었으며, 이를 반영하여 파쇄기 등을 이용하여 수확시기 줄기를 절단한 마늘을 굴취 및 수집하는 수집형 마늘 수확기(시작기)를 투입한 전과정 기계화 포장시험을 수행하였다. 관행구의 경우 종자준비(쪽분리선별기)→본밭준비(로터베이터·비료살포기)→제초제살포(동력분무기)→두둑성형·피복(휴립피복기)→파종(인력)→재배관리→수확(굴취기+인력)→줄기후처리(줄기절단기)로 작업체계를 구성하였으며, 각각의 작업공정별로 농가 보급이 활발한 농기계 외에는 관행 재배방법을 참고하여 인력을 투입하였다. 기계화구의 경우 종자준비(쪽분리선별기)→본밭준비(로터베이터·비료살포기)→두둑성형·피복·제초제(복합휴립피복기)→파종(파종기)→재배관리→줄기전처리(파쇄기)→수확(시작기)로 작업체계를 구성하였으며, 보급이 활발한 농기계 외에 트랙터용 파종기와 같이 보급 초기이거나, 개발중인 시작기(수집형 수확기)를 투입하여 마늘 재배 주요 작업공정 전과정에 농기계를 투입하였다. 관행구와 기계화구 소요 작업시간 분석을 통해 전과정 기계화 효과를 분석하였으며, 그 결과 마늘 생산 전과정기계화 기술은 관행 작업보다 노동력 92.7% 절감 효과가 있는 것으로 나타났다.