견의 총백혈구수 및 호산구수의 동시적계산을 위한 개량희석액에 관한 연구

이성호,이방환,이원창,Lee Sung Ho,Lee Bang Whan,Lee Won Chang 대한수의사회 1978 대한수의사회지 Vol.14 No.1

The study was conducted in an attempt to estimate the efficiency of various diluting fluids for eosinophil count to oxalated blood and EDTA blood of human and canine, and to prepare the improved diluting fluid adaptable to concurrent direct counts of eosi

Expression of Luteinizing Hormone (LH) Subunit Genes in the Rat Ovary

이성호,Lee, Sung-Ho The Korean Society for Reproductive Medicine 1998 Clinical and Experimental Reproductive Medicine Vol.25 No.2

흰쥐 난소에서의 Luteinizing hormonoe (LH) subunit 유전자 발현과 LH polypeptide의 존재를 조사하였다. 이를 위해 LH subunit들에 대한 역전사 중합효소연쇄반응 (RT-PCR)을 시행하고, 난소내 LH 함량을 방사면역측정법으로 정량하였다. 뇌하수체와 정소에서 공통적으로 존재하는 LH-$\beta$ subunit$(LH-\beta})$의 exon에 해당되는 primer를 사용하여 RT-PCR을 시행한 결과 흰쥐 난소에서도 뇌하수체, 정소와 같이 306 bp band가 확인되었고, 정소특이적인 exon에 해당되는 primer를 사용한 결과 정소와 난소에서 예상대로 428 bp band가 검출되었다. 또한 LH, FSH, TSH 그리고 hCG에서 공통적으로 발현되는 common $\alpha$-subunit $(C_\alpha)$의 전사물질도 PCRdml 의해 증폭되었다. 방사면역측정법에서는 LH standard curve와 난소추출물을 사용한 curve가 동일하게 sigmoid 형태를 보임으로서 흰쥐 난소내에 immunoreactive LH가 존재함이 증명되었다. 인위적으로 성적인 성숙을 유도한 PMSG 주사 동물에서 혈중 LH 수준은 주사 후 48시간에 preovulatory LH surge와 유사한 최고 수준을 나타냈으나, 난소내 LH 함량의 경우 주사 24시간 후부터 급격히 감소하여 주사 48, 72시간군까지도 낮은 수준이 유지되었다. 이 결과는 흰쥐 난소의 LH가 생리적으로 조절되고 그 조절방식이 뇌하수체에서와는 다를 가능성을 시사하는 것이다. 본 연구는 흰쥐의 난소에서 LH가 유전자가 발현됨을 최초로 보고한 것이며, LH의 경우 내분비적 경로 (endocrine; 뇌하수체로 부터의 LH)외에도 국부적 경로 (autocrine이나 paracrine; 난소내에서 합성되는 LH)를 통해 난소의 생리와 기능 조절을 담당함을 시사한다. The present study was performed to analyze the expression of LH genes in the rat ovary. Expression of LH subunit genes in the rat ovary was demonstrated by amplification of ovarian RNA by RT-PCR. The ovarian $LH_\beta$ transcripts contained at least two parts of the published cDNA structure, the pituitary exons 1, 2 and 3 and the part of testicular ex on 1 in the major trancripts form in rat testis. Using RIA, significant amount of LH-like molecules were detected in crude ovarian extracts, and the competition curves with increasing amount of tissue extracts were parallel with those of standard peptide, indicating that the ovarian immunoreactive LH-like material is similar to authentic pituitary LH molecule. The administration of PMSG to immature rats resulted in a sharp decrease of the ovarian LH contents after 24 h post-injection. In conclusion, these findings demonstrate that genes for LH subunits are expressed in the rat ovary, and suggest that LH can playa central role in regulation of female reproduction with both endocrine (by pituitary LH) and auto- and/or para-crine (by ovarian LH) manner.

진행 위암에서의 위 전절제술에 동반된 원위부 췌-비장 절제

이성호,김욱,송교영,김진조,진형민,박조현,전해명,박승만,안창준,이준현,Lee, Sung-Ho,Kim, Wook,Song, Kyo-Young,Kim, Jin-Jo,Chin, Hyung-Min,Park, Jo-Hyun,Jeon, Hae-Myung,Park, Seung-Man,Ahn, Chang-Jun,Lee, Jun-Hyun 대한위암학회 2007 대한위암학회지 Vol.7 No.2

Purpose: Routine pancreatico-splenectomy with total gastrectomy should no longer be considered as the standard surgical procedure for gastric cancer because of the lack of proven surgical benefit for survival. The aim of this study is to evaluate the clinicopathologic factors and the survival of patients with locally advanced gastric cancer and they had undergone combined pancreatico-splenectomy with a curative intent. Material and Methods: We retrospectively reviewed a total of 118 patients who had undergone total gastrectomy with distal pancreatico-splenectomy from 1990 to 2001. The patients were divided into 2 groups: 90 patients who were free from cancer invasion (group I), and 28 patients with histologically proven cancer invasion into the pancreas (group II). The various clinicopathologic factors that were presumed to influence survival and the survival rates were analyzed. Results: The rate of pathological pancreatic invasion was 23.7%. The tumor stage, depth of invasion, pancreas invasion, lymph node metastasis, lymph node ratio, curability and the hepatic and peritoneal metastasis were statistically significance on univariate analysis. Among these factors, the tumor stage, lymph node ratio and curability were found to be independent prognostic factor on multivariate analysis. The 5-years survival rates were 36.2% for group I and 13.9% for group II. The morbidity rate was 22.1%, and this included pancreatic fistula (5.1%), intra-abdominal abscess (4.2%) and bleeding (4.2%). The overall mortality rate was 0.8%. Conclusion: Combined distal pancreatico-splenectomy with total gastrectomy with a curative intent was selectively indicated for those patients with visible tumor invasion to the pancreas, a difficult complete lymph node dissection around the distal pancreas and spleen, and no evidence of liver metastasis or peritoneal dissemination.

청송약수를 이용한 두부응고에 관한 기초연구

이성호,Lee, Sung-Ho 대한화학회 2009 대한화학회지 Vol.53 No.3

This is the basic study of Cheong-song mineral waters(Dalgi and Sinchon) for a coagulant of Tofu. For comparison, $CaCl_2,\;CaSO_4,\;MgCl_2\;and\;MgSO_4$ are used as reference coagulants. The coagulability test was conducted by measuring the percentage transmittancy of filtered whey solution at 600 nm wavelength, in $80\;{^{\circ}C}$ and 10 mL of solution volume which composed of 2 mL of soy milk and coagulants. The coagulability test of mineral waters require more than an aliquot of 8 mL. Good result obtained by adding 0.4, 1, 0.8, 0.8 mL of reference coagulants and this is equivalent to the amount of 66.14, 232.83, 95.68, 78.88 mg of Ca and Mg respectively. The percentage transmittancy of filtered whey solution with added 8 mL of Dalgi and Sinchon mineral waters showed 50.74 and 58.52 respectively and the amount of mineral content of Ca and Mg equivalent to 2.41 and 2.48 mg respectively which is very lower value than the reference coagulants. This means mineral water has coagulation ability even in the low concentration.

흰쥐 자궁과 부정소에서의 Luteinizing Hormone (LH) 유전자 발현

이성호,이영기,Lee, Sung-Ho,Lee, Young-Ki 대한생식의학회 1999 Clinical and Experimental Reproductive Medicine Vol.26 No.2

Recent studies clearly demonstrated that the novel expression of LH gene in the rat testis, and suggested the local action of the LH-like molecule. The present study was performed to analyze the expression of LH genes in the rat accessory reproductive organs. Expression of LH subunit genes in the rat uterus and epididymis was demonstrated by reverse transcription-polymerase chain reaction (RT-PCR) and specific LH radioimmunoassay (RIA). The $LH_{beta}$ transcripts in these organs contained the published cDNA structure, the pituitary type exons 1-3, which encoded the entire $LH_{beta}$ polypeptide. Presence of the transcripts for the ${\alpha}$-subunit in the rat reproductive tissues were also confirmed by RT-PCR. In the LH RIA, significant levels of LH were detected in crude extracts from the rat ovary, uterus and epididymis. The competition curves with increasing amount of tissue extracts were parallel with those of standard peptide, indicating that the immunoreactive LH-like materials in these tissues are similar to authentic pituitary LH molecule. In rat epididymis, the highest amount of immunoreactive LH was detected in corpus area. Our findings demonstrated that the genes for LH subunits are expressed in the rat accessory reproductive organs, and suggested that these extrapituitary LH may act as a local regulator with auto and/or paracrine manner.

Zig-Zag 결선 및 Open-Delta 방식을 이용한 새로운 고조파 저감장치의 개발

이성호,김기성,유상봉,Lee, Sung-Ho,Kim, Gi-Sung,Yoo, Sang-Bong 한국조명전기설비학회 2005 조명·전기설비학회논문지 Vol.19 No.1

지금까지의 고조파 필터는 중성선 리액터와 지그재그결선의 영상 고조파 전류를 제거하였으나, 부하율에 따라 고조파 저감율이 변하여 경부하에서는 고조파 저감율이 낮아지는 특성이 있고, 중성선 리액터 과열로 중성선 단선 위험의 문제가 발생하였다. 본 Zig-Zag 결선 및 Open Delta 방식을 이용한 새로운 고조파 저감장치(HANOS)는 Zig-Zag 결선의 Core Block내에 추가로 Open Delta 방식을 결선하여 중성선에 연결함으로써 중성선에 흐르는 영상 고조파 전류가 과열 없이 안전하게 제거되는 것을 시험 분석한 결과로 증명하였다. The conventional harmonic filters to reduce zero harmonic current from neutral reactors and Zig-Zag connection, have several disadvantages of the decreased reduction rate of harmonics under a light load, because they have the load factor-dependent reduction rate of harmonics, and the risk of potential breaking in the neutral line by heated neutral reactor. Based Zig-Zag connection and Open-Delta mode, this new harmonic eliminating device (HANOS) adopts the combination of Zig-Zag connection and Open-Delta mode-the latter is additionally applied to the transformer's core block for connection to the neutral line. The results of this study demonstrated that the new device could eliminate safely zero harmonic current running in the neutral line without heating.

ICP-MS에 의한 Tungsten Matrix 중의 미량원소분석

이성호,Lee, Sung-Ho 대한화학회 2008 대한화학회지 Vol.52 No.6

아스코빅산에 의한 백금족 금속 용액에서의 Pd (Ⅱ) 의 침전 특성

이성호,김광락,백승우,강희석,유재형,정흥석 ( Sung Ho Lee,Kwang Rag Kim,Seung Woo Paek,Hee Suk Kang,Hong Suk Chung ) 한국공업화학회 1998 응용화학 Vol.2 No.1

High-level wastes contain significant quantities of platinum group metals such as palladium, ruthenium, and rhodium. These metals are used for catalytic process, electronic and electrochemical industries, anti-corrosion alloys, dental applications etc. The demand of noble metals continues to grow in a steady manner. Many techniques for separation of the platinum group metals have been studied. In this study, the precipitation characteristics of Pd(II) by ascorbic acid in a simulated platinum metal solutions was investigated.

청송 신촌약수의 수질특성에 관한 연구

이성호,Lee, Sung-Ho 대한화학회 2000 대한화학회지 Vol.44 No.4

주강제 노출형 철골주각부의 휨 성능에 관한 실험 연구

이성호,박형철,오보환,Lee, Sung Ho,Park, Hyung Chul,Oh, Bo Hwan 한국강구조학회 2005 한국강구조학회 논문집 Vol.17 No.1

기존의 주각부는 보강을 위하여 주각부의 가공에 많은 인력과 시간이 필요하게 되며 용접부가 과다하게 되어 베이스 플레이트에 변형이 발생하여 현장 설치시 앵커볼트와의 조립이 어려운 점등의 문제가 있다. 또한 상부구조의 응력분포와 변형 등을 평가하는 것에 있어서 중요한 의미를 가지는 주각의 고정도는 베이스플레이트의 강성부족, 앵커볼트의 느슨함, 베이스플레이트 하면(下面) 모르타르의 충진불량 등에 의해서 실제 해석상의 완전고정보다는 고정도가 저하된다. 본 연구는 기존의 주각구성 방법을 개선하기 위해 응력의 분포에 따라 베이스 플레이트의 두께조절이 가능한 주강(鑄鋼)을 사용하여 베이스플레이트의 강성을 크게하고, 주각부를 보다 간편하게 구성할 수 있는 방법을 도입하였다. 이러한 주각구성법은 철판을 절단하여 용접하는 기존의 형식을 탈피하여 주각부의 베이스플레이트를 응력의 분포에 맞춰 그 두께를 조절할 수 있는 주강제품으로 제작함으로써 주각부의 가공 및 용접량을 최대한 줄이고 베이스플레이트의 강성을 증대시키며, 응력의 흐름도 명확하게 할 수 있을 뿐만 아니라 시공의 간편성, 품질의 향상을 도모하는 것에 그 목적이 있다. 이러한 주강제 주각부의 개발을 위하여, 기존 주각부와 주강제 주각부에 대한 실험체를 제작하여 반복가력 시험을 통하여 내력과 강성을 파악하고, 피로 시험을 통하여 장기적인 사용성을 확인하였다. 이상의 실험을 통해 주강제 주각부와 기존의 주각부의 구조적 성능을 비교하였을 때 내력과 강성면에서 동등한 성능을 보유하고 있는 것으로 나타났고, 사용하중의 반복가력 평가에서도 주강제 주각부가 안정적인 거동을 하는 것으로 평가되었다. Manufacturing conventional column base plate requires much manpower and time. In this research, a new method for constructing column base plate is introduced to improve on conventional methods through the use ofcast steel that is available for adjusting base plate thickness and enlarging base plate stiffness. The main purposes include reducing welding work, enlarging base plate stiffness, and clarifying the stress flow. Also, construction convenience and improvement in quality can be expected. For developing this cast steel base plate, test specimens of conventional and cast steel base plates are made and tested to analyze strength and stiffness. Also, the efficiency for long-term use is checked by fatigue tests. From these comparative tests, cast steel base plates have the same strength and stiffness as conventional base plates.