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- 저자 : 이상권(Lee, Sang-Gwon) (검색결과 385 건)
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이상권,손정훈,최의성,이상기,Lee, Sang-Kwon,Sohn, Jung-Hoon,Choi, Eui-Sung,Rhee, Sang-Ki 생화학분자생물학회 1993 한국생화학회지 Vol.26 No.3
한국산 거머리인 Hirudo nipponia와 Whitmania sp.의 추출액으로 trypsin, factor Xa, thrombin에 대한 저해활성을 조사하였다. Hirudo nipponia로부터 얻은 추출액은 $20{\mu}g/ml$ 농도에서 이 효소들의 활성을 완전히 저해하는 반면 Whitmania sp.의 추출액은 동일조건하에서 trypsin만을 저해하였다. 이 Hirudo nipponia의 추출물로부터 항thrombin 인자를 분리 정제하기 위하여 anion exchange chromatography, size exclusion chromatography 및 HPLC를 이용하였고 최종적으로 세 종류의 단백질(I-A,I-B,I-C)을 얻었다. 이 중 두 종류 단백질(I-A, I-B)의 아미노말단 서열을 부분적으로 분석한 결과 이 단백질이 주로 산성 아미노산잔기로 구성되어 있으며 heparin cofactor II의 일부분과 유사성이 있음이 발견되었다. Two Korean leech species, Hirudo nipponia and Whitmania sp., were examined for the presence of anticoagulant activity. Crude extracts of the leech head parts were fractionated by ethanol and the anticoagulant activity of these extracts was assessed with respect to the inhibition of thrombin, factor Xa and trypsin using the chromogenic substrate assay. While the extract from Hirudo nipponia inhibited all three serine proteases employed in the assay, that of Whitmania sp. inhibited only trypsin, but not thrombin and factor Xa. Thus, the extract from Hirudo nipponia which shows antithrombin activity was subjected to further purification. Three steps of column chromatography including gel filtration on Superose 12 column, anion exchange on Mono-Q and reverse phase HPLC on C-8 column could resolve 3 antithrombin activity peaks from anti-trypsin and anti-factor Xa activities, indicating the presence of multiple forms of thrombin-specific inhibitors in Hirudo nipponia. N-terminal amino acid sequences of the two of these forms showed a limited degree of homology with a portion of heparin cofactor II.
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이상권,김상필,송현,김종욱,송명근,이재원,Lee, Sang-Gwon,Kim, Sang-Pil,Song, Hyun,Kim, Jong-Ook,Song, Meung-Gun,Lee, Jae-Won 대한흉부심장혈관외과학회 1999 Journal of Chest Surgery Vol.32 No.8
Background: To secure a rapid and safe approach which is at the same time cosmetically appealing, we employed the right anterolateral thoracotomy incision for repair of atrial septal defects and valvular heart diseases in the adult. Material and method: Between October 1989 and June 1998, 44 adult patients underwent open heart surgery through right anterolateral thoracotomy at our institution. Operative time, cardiopulmonary bypass time, aortic cross clamp time, blood loss until chest tube removal, length of ICU stay, days to discharge, and survival were compared with those that received cardiac surgery via conventional sternotomy. Result: No significant differences were observed between the two groups. There was no death and no additional morbidity directly related to this approach. Cosmetically satisfying results were obtained with safety using the right anterolateral thoracotomy approach. Conclusion: Our data show that the right anterolateral thoracotomy approach is a safe alternative to conventional median sternotomy as it offers excellent exposure and aesthetically more acceptable wounds while not adding on to the operative risks.
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이상권,송주동,김강미,김종민,이상률,유영현,박영철,Lee, Sang-Kwon,Song, Ju-Dong,Kim, Kang-Mi,Kim, Jong-Min,Lee, Sang-Yull,Yoo, Young-Hyun,Park, Young-Chul Korean Society of Life Science 2007 생명과학회지 Vol.17 No.9
The topoisomerase II inhibitor etoposide causes an accumulation of DNA double strand breaks within the nuclei of cells. In this study, we investigated the effect of etoposide on the cell growth and apoptosis of human RPE cells. Etoposide evoked a significant inhibition of cell growth, and also induced DNA fragmentation in ARPE-19 cells. In addition, etoposide significantly up-regulated the expression of heme oxygenase-1 (HO-1), which is a stress-responsive protein and is known to play a protective role against the oxidative injury. And, etoposide-induced HO-1 expression was affected by the ROS scavenger N-acetyl cysteine. We also used oligonucleotides interfering with HO-1 mRNA (siRNA) for the inhibition of HO-1 expression. Interestingly, knock-down of the HO-1 gene significantly increased the level of DNA fragmentation in etoposide-treated ARPE-19 cells. In conclusion, these results suggest that up-regulated HO-1 plays as an anti-apoptotic factor in the process of apoptosis of ARPE-19 cells stimulated by etoposide.
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소아 백혈병에서 중추신경계 예방 치료에 따른 백질뇌병증:자기공명영상 소견
이상권,김태헌,김용주,강덕식,Lee, Sang-Gwon,Kim, Tae-Heon,Kim, Yong-Ju,Gang, Deok-Sik 대한영상의학회 2001 대한영상의학회지 Vol.45 No.4
Purpose: To evaluate the MR imaging findings and the usefulness of MR imaging in the diagnosis and follow-up of leukoencephalopathy following CNS prophylaxis therapy in pediatric leukemia. Materials and Methods: We retrospectively evaluated the MR imaging findings of eight children with white matter abnormalities on MR out of seventeen acute leukemic patients with various neuropsychiatric symptoms who received intrathecal methotrexate administration, with or without cranial irradiation. In all cases, initial MR was performed within a week of the onset of neuropsychiatric symptoms. Follow-up MR was performed one to sixteen months after initial study, and the MR imaging findings were compared with the initial findings. Results: The initial MR imaging findings were classified into three categories: focal or multifocal white matter abnormalities (3/8), and diffuse white matter abnormalities without enhancement (3/8), and diffuse white matter abnormalities with enhancement (2/8). At follow-up MR, diffuse or focal atrophic changes were noted in all children. White matter abnormalities improved in two out of three patients with focal or multifocal white matter abnormalities. In five with diffuse white matter abnormalities, the extent of these showed no significant change, but contrast enhancement was markedly reduced in two children in whom diffuse white matter abnormalities with enhancement had been demonstrated. Conclusion: In pediatric leukemia, the MR imaging findings of leukoencephalopathy following CNS prophylaxis therapy are variable, but are specific with the clinical history of neuropsychiatric symptoms after intrathecal methotrexate administration, with or without cranial irradiation. The MR imaging is valuable in the diagnosis and follow-up of leukoencephalopathy following CNS prophylaxis therapy in pediatric leukemia.
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위선암에서 Matrix Metalloproteinase-2, Cathepsin D 및 E-cadherin의 발현
이상권,박승철,김원우,김욱,이교영,전해명,김응국,고용복,Lee Sang Kuon,Park Seung Cheol,Kim Won Woo,Kim Wook,Lee Kyo Young,Jeon Hae Myung,Kim Eung Kook,Koh Yong Bok 대한위암학회 2002 대한위암학회지 Vol.2 No.3
Purpose: The prognosis of gastric cancer depends on the depth of invasion, lymph-node metastasis, invasion to adjacent tissues, and distant metastasis. Recently, it is known that tumor-associated proteases and adhesion molecules have been shown to play a relevant role in the process of progression and metastasis. The purpose of our study was to demonstrate the value of MMP-2 (matrix metalloproteinase), cathepsin D and E-cadherin as prognostic factors. Materials and Methods: In this study, formalin-fixed, paraffin-embedded tissue blocks from 69 patients with gastric cancer were immunohistochemically studied using antibodies to MMP-2, cathepsin D, and E-cadherin, and their expressions were analyzed according to the pathologic stage, lymph-node metastasis, histological differentiation, and patient survival. The medical records of these patients were retrospectively reviewed. Results: Increased expression of MMP-2 significantly correlated with advanced pathologic stage (P=0.026). Patients with lymph-node metastasis also had increased expression of MMP-2. Those patients with increased expression of MMP-2 showed a poorer survival; nevertheless, it was not statistically significant. Increased expression of cathepsin D significantly correlated with advanced pathologic stage (p=0.029). However, no correlation was observed between advanced pathologic stage and either lymph-node status or histological differentiation. Patients with increased expression of cathepsin D had a poorer survival, but that result was not statistically significant. No association was found between reduced expression of E-cadherin and pathologic stage, lymph-node status, or histological differentiation. Also, no correlation was found between the expression of E-cadherin and survival. In addition, when a combination of MMP-2 and cathepsin D expressions was analyzed, if both were negative, the survival seems to be longer, but it was not statistically significant. Conclusions: In patients with gastric cancer, expressions of MMP-2 and cathepsin D correlated with tumor stage; therefore, they may be considered as prognostic factors.
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