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      • KCI등재

        저습지 출토 수침고목재의 미생물 분해 특징 및 미생물 군집 분석

        김민석,김종식,차미영,임주원 한국문화재보존과학회 2023 보존과학회지 Vol.39 No.4

        Anatomical and chemical changes due to microbial decay and microbial communities inthree waterlogged archaeological woods, namely Dongnim 1, Dongnim 2, and Dongnim 3, excavatedfrom the wetland of the Dongnim-dong archaeological site in Gwangju, were investigated. Dongnim1−3 exhibited a maximum moisture content approximately 6 to 8 times higher than that of undecayedrecent wood, and there was no correlation with the burial period. Chemical analysis revealed an increasein ash and hot water extractive content in Dongnim 1−3 compared to undecayed recent wood, whilethe solvent extractive content tended to decrease. Additionally, there was a significant decrease inholocellulose content with a relative increase in lignin concentration in Dongnim 1−3. Microscopicobservations showed erosion bacteria decay and soft rot decay in Dongnim 1−3. The microbialcommunity analysis of Dongnim 2 and 3 revealed the dominance of Ascomycota (98% to 99%) inthe fungal community and Proteobacteria (34% to 55%) as the predominant bacterial phylum. Nonetheless, significant differences in microbial community structure and diversity were observedbetween Dongnim 2 and Dongnim 3.

      • SCIEKCI등재

        Antifungal Activity of Eucalyptus-Derived Phenolics Against Postharvest Pathogens of Kiwifruits

        Oh, Soon-Ok,Kim, Jung-A,Jeon, Hae-Sook,Park, Jong-Cheol,Koh, Young-Jin,Hur, Hyun,Hur, Jae-Seoun The Korean Society of Plant Pathology 2008 Plant Pathology Journal Vol.24 No.3

        Antifungal activities of natural substrances from Eucalyptus darlympleana, E. globules, E. gunnii and E. unigera were evaluated against postharvest pathogens of kiwifruits, Botrytis cinerea, Botryosphaeria dothidea, and Diaporthe actinidiae, to screen effective natural substances as an alternative to chemical fungicides. Methanol extract of the Eucalyptus trees showed strong antagonistic activity against the pathogenic fungi. Among them, E. unigera and E. darlympleana effectively inhibited mycelial growth of the pathogens. For chemical identification of the antifungal substances, the methanol extract of E. darlympleana leaves was successively partitioned with $CH_2Cl_2$, EtOAc, n-BuOH and $H_2O$. Among the fractions, $CH_2Cl_2$ and n-BuOH showed strong inhibitory activity of mycelial growth of the fungi. Five compounds were isolated from EtOAc and n-BuOH fractions subjected to $SiO_2$ column chromatography. Two phenolic compounds(gallic acid and 3,4-dihydroxybenzoic acid) and three flavonoid compounds(quercetin, quercetin-3-O-$\alpha$-L-rhamnoside, quercetin-3-O-$\beta$-glucoside) were identified by $^1H$-NMR and $^{13}C$-NMR spectroscopy. Among them, only gallic acid was found to be effective in mycelial growth and spore germination of B. cinerea at relatively high concentrations. The results suggest that gallic acid can be a safer and more acceptable alternative to current synthetic fungicides controlling soft rot decay of kiwifruit during postharvest storage.

      • KCI등재

        Antifungal Activity of Eucalyptus-Derived Phenolics Against Postharvest Pathogens of Kiwifruits

        오순옥,김정아,박종철,고영진,허현,허재선,전해숙 한국식물병리학회 2008 Plant Pathology Journal Vol.24 No.3

        Antifungal activities of natural substrances from Eucalyptus darlympleana, E. globules, E. gunnii and E. unigera were evaluated against postharvest pathogens of kiwifruits, Botrytis cinerea, Botryosphaeria dothidea, and Diaporthe actinidiae, to screen effective natural substances as an alternative to chemical fungicides. Methanol extract of the Eucalyptus trees showed strong antagonistic activity against the pathogenic fungi. Among them, E. unigera and E. darlympleana effectively inhibited mycelial growth of the pathogens. For chemical identification of the antifungal substances, the methanol extract of E. darlympleana leaves was successively partitioned with CH2Cl2, EtOAc, n-BuOH and H2O. Among the fractions, CH2Cl2 and n-BuOH showed strong inhibitory activity of mycelial growth of the fungi. Five compounds were isolated from EtOAc and n-BuOH fractions subjected to SiO2 column chromatography. Two phenolic compounds (gallic acid and 3,4-dihydroxybenzoic acid) and three flavonoid compounds (quercetin, quercetin-3-O-α-L-rhamnoside, quercetin-3-O-β- D-glucoside) were identified by 1H-NMR and 13C-NMR spectroscopy. Among them, only gallic acid was found to be effective in mycelial growth and spore germination of B. cinerea at relatively high concentrations. The results suggest that gallic acid can be a safer and more acceptable alternative to current synthetic fungicides controlling soft rot decay of kiwifruit during postharvest storage.

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