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Macrophages로부터 IL-1β 분비 및 전사에 있어서 한국산 겨우살이 추출물 M11C (non-lectin components)의 효과
장성호,전명하,강태봉,문세환,이준호,성낙술,이성태,김종배,허억,Chang, Sung Ho,Jun, Myung Ha,Kang, Tae Bong,Mun, Se Hwan,Lee, Jun Ho,Seong, Nak Sul,Lee, Sung Tae,Kim, Jong Bae,Her, Erk 대한면역학회 2001 Immune Network Vol.1 No.2
Background: Korean mistletoe (Viscum album) extract has been found to posses immunostimulatory activity. In this study, Korean mistletoe extract, M11C (non-lectin components), was used to know whether this extract might activate mouse peritoneal macrophages to produce interleukin $1{\beta}$ (IL-$1{\beta}$). Methods: Hemagglutination assay was carried out to examine whether M11C contained a lectin or not. To know the effect of M11C on the production of IL-$1{\beta}$, the macrophages were treated by the M11C, and then collected the supernatant (M11C stimulated macrophages-conditioned media; MMCM). MMCM was analyzed for the IL-$1{\beta}$ quantification and mRNA expression by means of ELISA and RT-PCR, respectively. Results: Maximum effective dose and time of M11C on IL-$1{\beta}$ production from macrophages were $20{\mu}g/m{\ell}$ and 8 hours, respectively. This ELISA data was reconfirmed by immunoblotting assay. indicating that M11C is a good candidate for an immunomodulator. The dose and time dependent effects of M11C on the expression of IL-$1{\beta}$ mRNA from macrophages was also shown in expression of mRNA detected by RT-PCR. Treatment dose and time for the maximum expression of IL-$1{\beta}$ mRNA were $20{\mu}g/m{\ell}$ and 4 hours, respectively. Maximum gene expression of IL-$1{\beta}$ was much earlier than maximum production of it. Conclusion: As results, Korean mistletoe extract, M11C, may be used for an immunomodulator. This will be able to make up for and solve the problems caused by existent immunoagent with many adverse effects through many other studies in future including one molecule extraction.
한국산 겨우살이 추출물 M11C(렉틴 구성물질)가단구세포의 TNF-α 유전자 발현유도 및 분비에 비치는 효과
허억 한국약용작물학회 2007 韓國藥用作物學會誌 Vol.15 No.1
It is well-known that Korean mistletoe (Viscum album) extract has an immune activity and anticancer effect. In this study, Korean mistletoe extract, M11C (non-lectin components), was used to examine whether this extract might activate human peripheral monocyte to produce tumor necrosis factor-α (TNF-α). To examine the effect of M11C on the production of TNF-α from monocyte, the monocyte were stimulated by the M11C, and then collected the supernatant (M11C stimulated monocyte-conditioned media; MCM). MCM was treated to the TNF-α sensitive L929 cells, and then L929 cytotoxicity was measured by means of MTT. MCM had cytotoxic effect on L929. And the cytotoxic effect of MCM on L929 was almost abolished by anti-TNF-α antibody. These data indicated that MCM contained TNF-α, suggesting the TNF-α generation from M11C-stimulated monocyte. This suggestion was confirmed from the data that TNF-α was highly detected in MCM by immunoblotting technique. M11C effect on TNF-α production from monocyte was in the dose and stimulating time dependent manners. Also the effect of M11C on the expression of TNF-α mRNA from monocyte was shown in the dose and stimulating time dependent manners. As a result, Korean mistletoe extract, M11C, could be used for an immunostimulator.