1,2-IND/Zn를 이용한 ninhydrin으로 처리한 지문의 후속 증강 연구

박은지(Eun-Jee Park),정하나(Ha-Na Jung),홍성욱(Sung-Wook Hong) 한국콘텐츠학회 2018 한국콘텐츠학회논문지 Vol.18 No.5

종이에 유류된 지문을 현출하기 위하여 전통적으로 사용되는 ninhydrin은 종종 배경색에 따라 사용이 제한되고 시약반응 환경에 따라 현출효과가 달라진다. 그러므로 최근에는 배경색에 제한이 없는 1,2-indanedione(1,2-IND) 등을 사용하고 있다. 이에, 본 연구는 지문 현출을 목적으로 ninhydrin을 처리한 종이에 1,2-IND/Zn를 후속 처리하였을 때 지문의 증강 여부를 확인하고자 하였다. 이를 위하여 시험관 내에서 아미노산과 ninhydrin 시약이 이미 반응한 용액에, 1,2-IND/Zn와 DFO를 추가했을 때의 반응을 관찰하고, ninhydrin만 처리한 지문과 ninhydrin을 처리한 후 1,2-IND/Zn 또는 DFO를 처리한 지문의 현출정도를 비교하였다. 그리고 ninhydrin을 처리한 종이의 보관 기간에 따른 1,2-IND/Zn 증강 효과를 관찰하였다. 그 결과, ninhydrin으로 처리한 종이 내 지문을 1,2-IND/Zn로 처리하였을 때 지문이 증강되었으며, 최대 6개월이 지난 시험지에서도 지문증강효과를 관찰할 수 있었다. Ninhydrin traditionally used to develop fingerprints on paper is often limited in its use depending on background color and its enhancement effect differs depending on reagent reaction condition. Recently 1.2-indanedione(1,2-IND), etc. which has no limitation of background color has been used to visualize the fingermark on paper, Therefore, this study was carried out for enhancement of fingerprints after 1,2-IND/Zn treatment on paper treated with ninhydrin. Comparison between 1,2-IND/Zn reaction after ninhydrin treatment and single ninhydrin reaction were conducted under various methods. Result shows the fingerprints were enhanced when ninhydrin-treated fingerprints were treated with 1,2-IND/Zn, and their enhancement was observed even after 6 months.

염화아연(Zinc Chloride)과 액화질소를 사용한 닌히드린(Ninhydrin) 형광지문의 증강 효과 비교 및 실무적 사용을 위한 새로운 냉각방법에 관한 연구

전수연,김창용,김미소,유제설 한국융합학회 2020 한국융합학회논문지 Vol.11 No.6

We identify the enhancement effects of zinc chloride and liquid nitrogen on low quality ninhydrin-developed fingerprint and we wanted to find out another cooling methods available for ninhydrin/Zn fingerprint enhancement besides the liquid nitrogen, which is hard to access. Artificial sweat was used to make the same level of fingerprints, and fingerprints developed by each technique were evaluated by SWGFAST standard and compared the average score and standard deviation. As a result of the experiment, ninhydrin/Zn-liquid nitrogen got 3.2 and 3.3 as average scores that enough to identify an individual such as 1,2-IND/Zn or DFO. Also, experiments using dry ice instead of liquid nitrogen as a way of the ninhydrin/Zn-developed fingerprints enhancement resulted in 3.0 and 2.9 as average scores, therefore, it was confirmed that dry ice is also a sufficient cooling method to be considered in practice in the field of forensic science. 법과학 분야에서 닌히드린(ninhydrin)은 다공성 표면 증거물에 남겨진 잠재지문을 현출하기 위해 사용하는 대표적인 시약이다. 하지만 지문 유류물의 양이 적은 잠재지문을 닌히드린으로 현출하면 개인식별을 하기에 충분하지 않은 지문이 현출될 가능성이 있다. 본 연구에서는, 이와 같이 품질이 낮은 닌히드린 현출 지문을 증강하기 위해 사용하는 염화아연과 액화질소의 효과를 알아보고, 쉽게 접하기 힘든 액화질소 외에 닌히드린/Zn 지문에 적용할 수 있는 또 다른 냉각방법이 있는지 찾아보고자 하였다. 동일한 수준의 지문을 제작하기 위해 인공 땀을 사용하였고, 각 기법으로 현출한 지문을 SWGFAST 기준을 통해 평가받아 평균과 표준편차를 비교하였다. 실험 결과, 닌히드린/Zn-액화질소는 3.2, 3.3점의 평균점수를 받아 1,2-IND/Zn, DFO와 마찬가지로 개인식별이 가능한 수준의 증강 효과를 보였다. 또한, 닌히드린/Zn 지문을 증강하는 방법으로 액화질소 대신 드라이아이스를 사용한 실험에서는 3.0, 2.9점의 평균점수가 나와, 실무적으로 충분히 고려해볼 수 있는 냉각 방법이라는 것을 확인할 수 있었다.

잠재지문 검출제로서 Ninhydrin 유도체들과 Glycine 과의 반응성에 관한 분자 홀로그래픽적인 QSPR분석

성낙도,김세곤,장석찬,조윤기,황태연,박성우 한국분석과학회 2007 분석과학 Vol.20 No.4

To search the ninhydrin derivatives that have high chromogenic and fluorogenic properties, molecularninhydrin analogues as latent fingerprint detector were derived and investigated quantitatively. The εLUMO(e.v.) energy of ninhydrin molecule was an important factor to reactivity of ninhydrin. And, it is suggestedthat the nucleophilic reaction by orbital-controlled reaction from the frontier molecular orbital (FMO) interactionbetween glycine and ninhydrin derivatives was more superior than that of electrophilic reaction by chargedcontroled reaction. The analytical results in atomic contribution maps also shows that the reactivity of ninhydrinsugested by HQSPR and QSPR model that the 5,6-dinitroninhydrin molecule would increase the reactivity asmuch as three times as compared to none substituted ninhydrin molecule.

Rapid selection of theanine-rich green tea (Camellia sinensis L.) trees and metabolites profiling by Fourier transform nearinfrared (FT-IR) spectroscopy

송현진,김용덕,정미진,안명숙,김석원,유장렬,최명석 한국식물생명공학회 2015 Plant biotechnology reports Vol.9 No.2

A simple and rapid ninhydrin-based colorimetric method was efficiently developed, and metabolites profiling of tea tree (Camellia sinensis L.) leaf extracts was carried out by Fourier transform near-infrared (FT-IR) spectroscopy. The tea extracts of Hadong region exhibited a wide range of variations in their theanine contents. In general, theanine and other amino acids were higher in classified theanine-rich lines than that of theanine-poor lines. Among selected theanine-rich lines, the theanine levels reached a maximum of 15.3 mg/g for line TR92, a value 51-fold more than that found in theanine-poor TP23 and TP156 lines. Therefore, ninhydrin-based colorimetric method can be efficiently adopted for the selection of theanine-rich plants thereby enabling selection of plants dependent on their chemical constituents. FT-IR spectroscopy analysis was conducted in 15 tea tree lines as theanine-rich 8 lines and -poor 7 lines. The spectral data were analyzed by principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA). PCA and PLS-DA could successfully discriminate theanine-rich and -poor lines. The quantitative prediction modeling of total amino acids and theanine from tea tree leaf lines was established using partial least square regression algorithm from FT-IR spectra. The regression coefficients (R2) between predicted values and estimated values of total amino acids and theanine were 0.976 and 0.992, respectively. These results showed that quantitative predictions of total amino acids and theanine were possible from FT-IR spectra of tea tree leaf lines with higher accuracy. These results suggest that the prediction system established in this study could be applied as a rapid selection of theanine-rich lines of tea tree.

Selective Methylation of the Ninhydrin-Phenol Adducts with I₂ in MeOH

Na, Jeong-Eun,GowriSankar, Saravanan,Lee, Sang-Ku,Kim, Jae-Nyoung Korean Chemical Society 2004 Bulletin of the Korean Chemical Society Vol.25 No.4

아미노산 반응시약을 이용한 충격흔 현출에 관한 연구

임효수,송예은,이은비,이상윤,김진표,박남규,서영일 한국법과학회 2016 한국법과학회지 Vol.17 No.2

특수 종이 표면에서 ninhydrin 계열 시약의 적용 및 효과성 비교에 관한 연구

김청(Chung Kim),조형우(Hyeong-Woo Cho),고현서(Hyun-Seo Koh),유제설(Je-Seol Yu) 한국콘텐츠학회 2016 한국콘텐츠학회논문지 Vol.16 No.9

해외의 연구들에 의하여 몇 가지 종이류에서는 ninhydrin 및 유사체 시약들의 잠재지문 현출 효과가 좋지 않다고 알려져 있는 바, 본 연구에서는 우리나라에서 사용되는 특수지에 대해 우리나라 실험 조건에서의 ninhydrin 계열 시약들의 효과는 어떤지 알아보았다. 이전 연구들을 토대로 6종의 종이류를 선택하였으며, 이들 종이류에 남겨진 잠재지문을 ninhydrin 계열 시약들의 4가지 기법을 적용하여 현출한 뒤 비교하였다. 실험 결과, 6종의 종이류 중에서 신문지가 실험에서 사용된 잠재지문 현출 기법들의 효과가 가장 떨어지는 표면이었으며 시약 중에서는 `1.2-IND/Zn`가 전체 표면에서 가장 높은 대조비의 지문을 현출해 내어 가장 뛰어난 시약으로 나타났다. 실험 결과를 토대로 우리나라 현장에서 발견된 특수지의 경우에 잠재 지문 현출 시약으로 `1.2-IND/Zn`를 우선적으로 사용하는 것이 좋다고 판단할 수 있었다. Some previous studies had proven that ninhydrin or its analogues qre not effective in developing fingermarks on some type of paper. This study has evaluated the efficiency of four reagents for detecting latent fingermarks on six unusual paper surfaces. Among the six types of paper surfaces, none of the reagents worked on the newspaper. The 1.2-IND/Zn developed fingermarks on most of the surfaces tested.

Optimization of the ninhydrin reaction and development of a multiwell plate-based high-throughput proline detection assay

Lee, Mi Rha,Kim, Cheol Soo,Park, Tusan,Choi, Young-Soo,Lee, Kyeong-Hwan Elsevier 2018 Analytical Biochemistry Vol.556 No.-

<P><B>Abstract</B></P> <P>We developed a high-throughput technique for highly sensitive measurement of trace amounts of proline, an indicator of drought stress in plants, using an optimized proline-ninhydrin reaction. In order to do this, proline detection time was minimized by omitting phosphoric acid from the ninhydrin reagent. Chromophore extraction using toluene was also omitted, thus lowering the risks to environment and human health, and allowing the use of readily available polystyrene plates. Proline detection sensitivity was assessed based on the concentration of sulfosalicylic acid in the solution, which indicated that 1% sulfosalicylic acid yielded the best sensitivity and linearity. These findings were applied to a multiwell plate-based multiplex analysis using a dry oven for the simultaneous analysis of a large number of drought-stressed plant samples with trace amounts of proline. The results showed that proline could be effectively detected in plants grown in soil with water content under 5%, demonstrating its potential for diagnosing drought early. The proposed multiwell plate-based multiplex assay is expected to be useful in manifold agricultural applications.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The proline-ninhydrin reaction was optimized for detecting trace proline in plants. </LI> <LI> Toluene was omitted in the reaction mixture, lowering environmental/human risk. </LI> <LI> A polystyrene multiwell-plate-based assay was developed. </LI> <LI> The assay allowed sensitive detection in actual, low-volume plant samples. </LI> </UL> </P>

A Simple Method to Determine Amino Acid Leakage and Germination Capabilities from Single Radish (Raphanus sativus L.) and Chinese Cabbage (Brassica rapa ssp. pekinensis) Seeds

민태기,홍바른 한국원예학회 2013 Horticulture, Environment, and Biotechnology Vol.54 No.3

The viability of radish (Raphanus sativus L.) and Chinese cabbage (Brassica rapa ssp. pekinensis ) seeds were evaluated using a seed leachate treated ninhydrin reagent that develops color from amino acid leakage. Three different cultivars of artificially aged radish and Chinese cabbage seeds were used for this purpose. The radish and Chinese cabbage seeds were individually soaked in distilled water and treated with the ninhydrin reagent. A clearly distinct purple color was observed for the treated seed leachate, with intensities that varied depending on the seed quality. Depending on the intensity, the color could be classified into four separate fractions: colorless, weak, medium or strong. Color absorbance values were classified into 9 fractions for radish seeds and 11 fractions for Chinese cabbage seeds. Subsequently, a germination test was performed with the seeds in any given fraction (classified by color intensity and absorbance) and correlations between germination and color intensity or absorbance were evaluated. In a test of three cultivars of artificially aged radish and Chinese cabbage seeds, the relationship between percent germinated and color intensity appeared to have a very high negative correlation in linear regression, with r2 = 0.97for radish seeds and r2 = 0.95 for Chinese cabbage seeds. Similarly, the relationship between the percent germinated and the absorbance had a high negative correlation, with r2 = 0.90 for radish seeds and r2 = 0.94 for Chinese cabbage seeds. In conclusion, the simple detection of amino acid leakage with ninhydrin reagent was successful at assessing individual seed quality, as high correlations between germination and color intensity/absorbance were revealed.

이중성 지문시약으로서 Ruhemann’s Purple 화합물과 그 금속이온착 화합물의 생성 반응성 및 DNA분자 보호 가능성

홍주은,최미정,성낙도 한국과학수사학회 2018 과학수사학회지 Vol.12 No.2

이중성 지문시약으로서 ninhydrin 유도체(N1-N6)와 아미노산 분자들(A1-A5)과의 반응생성물(Ruhemann’s Purple) 및 그 금속(M)이온 착 화합물의 반응성에 관련한 전산 화학적인 접근으로부터 유전자(DNA) 보호 가능성을 정량적으로 검토하였다. Ninhydrin 유도체와 아미노산 분자사이의 FMO에너지 도표로부터 리간드(L) 화합물(Schiff-base; Ruhemann’s Purple)로서 1,8-diazofluoren-9-one (L4) 분자의 생성이 우세하였으나 공명(resonance)현상으로 dipyridoimidazol-6-one (L6) 분자가 ZnCl2와 가장양호한 금속 착 화합물화 반응성을 나타내었다. Schiff-base (L) 생성반응 중 탈수된 물 분자들은수화된 금속 이온([M(H2O)n]z+)으로 전환되어 DNA 분자를 분해한다. 그러므로 DNA분자를 보호할수 있는 진정한 의미의 이중성 지문시약은 수화된 금속이온이나 또는 그의 aqua Schiff-base 착([M(L) (H2O)2Cl]+2) 화합물(M: L =1:1)이 아니라, 수화된 금속이온이 존재하지 않는 M: L =1:2 비율의 Schiff-base 착([M(L2) ]+2) 화합물이었다. 결론적으로 이들 수화되지 않은 착 화합물(M: L =1:2)들이 장 파장대(500-600 nm)의 차별화된 최대흡수 파장(λmax.)을 나타냄으로서 DNA 분자가 보호될 수 있는 신규 이중성 지문시약으로서 역할을 기대할 수 있었다.