리그닌/PVA 나노섬유 웹의 수분 특성 및 생분해성 평가

송유정 ( Youjung Song ),이은실 ( Eunsil Lee ),이승신 ( Seungsin Lee ) 한국의류학회 2017 한국의류학회지 Vol.41 No.3

The biodegradation and water absorption properties of lignin/poly(vinyl alcohol) (PVA) nanofibrous webs are investigated. Lignin/PVA nanofibrous webs containing 0, 50, and 85wt% of lignin were prepared via an electrospinning process to observe the effect of the lignin concentration on the biodegradability and water absorption properties of lignin/PVA nanofibrous webs. The morphology of the materials was examined by field emission scanning electron microscopy (FE-SEM) and atomic force microscopy (AFM). To understand the wetting behavior and hydrophilic nature of the electrospun lignin/PVA nanofibrous webs, the water absorbency, contact angle, and water uptake were examined. The enzymatic degradation of lignin/PVA nanofibrous webs was investigated using laccase by measuring total organic carbon (TOC) concentration over a course of 50 days. Water drops were absorbed immediately into all of the specimens. The water uptake of lignin/PVA nanofibrous webs increased as the amount of PVA in the lignin/PVA hybrid webs increased. The enzymatic degradation experiment indicated that the inherent biodegradability of lignin was retained after its transformation into nanofibers. Our findings imply that blending these two types of polymers is promising because it can lead to the development of a new range of multifunctional materials such as antimicrobial absorbent nanotextiles based on sustainable biopolymers.

BMP법에 의한 리그닌의 혐기성 분해 및 GC와 GC/MS을 이용한 리그닌 분해산물 측정

김석구 유기성자원학회 2008 유기물자원화 Vol.16 No.3

The traditional view of the fate of lignin under anaerobic conditions is that it is recalcitrant because molecular oxygen is required for depolymerization. The presence of lignin is apparently the most important factor affecting the biodegradability of ligneous materials. The initial step in the degradation of ligneous material to smaller intermediates is catalyzed by enzymes secreted by microorganisms and is generally regarded as the rate limiting step in the microbial mineralization of organic matter. Biochemical methane potential (BMP) test, typically used to assess anaerobic biodegradability of liquid wastes with added nutrients and bacteria, have been adapted to assess initial biodegradation of ligneous material under anaerobic conditions. A method based on selective inhibition of microorganism activity, by 3% toluene, has been used to measure using the initial degradation rate of ligneous material and the accumulation of lignin-derived compounds.

Oxidative biodegradation of single-walled carbon nanotubes by partially purified lignin peroxidase from Sparassis latifolia mushroom’

Gayathri Chandrasekaran,신현재,최수경,이영철,김근중 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.32 No.-

Two types of carbon nanotubes (usually single-walled carbon nanotubes (SWCNTs) and multi-walled carbon nanotubes (MWCNTs)) have been intensively focused on academic researches and mass-produced for wide applications such as composite materials, biosensors, and drug delivery systems. However, due to oxidative stress-dependent and physically-induced cellular toxicity of CNTs, many efforts to render biocompatible and biodegradable properties in CNTs have been highlighted. Thus, taking into the consideration of exposure in human health and the environment, biodegradation of CNTs as a potential disposal is highly addressed. In this study, lignin peroxidase (LiP) was isolated and partially purified from the fruiting bodies of the edible mushroom Sparassis latifolia (S. latifolia). The biodegradation of raw grade and thermally-treated carboxylated SWCNTs (denoted as ASA and AST) with this enzyme was investigated, prior to more biodegradation-resistant MWCNTs. The interactions between the SWCNTs and LiP were investigated using various techniques, and the intermediate by-products of the LiP degradation were identified. Our findings demonstrated that both ASA and AST were efficiently degraded by LiP where the producing radicals by the LiP played a critical role in the biodegradation of SWCNTs. The final degraded products were confirmed with the generation of CO2 gas. Conclusively, the low extraction cost of partially purified enzyme from mushrooms can make this approach a promising alternative in environmental bioremediation as a practical application.

Oxidative biodegradation of single-walled carbon nanotubes by partially purified lignin peroxidase from Sparassis latifolia mushroom

Gayathri Chandrasekaran,최수경,이영철,김근중,신현재 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.5

Two types of carbon nanotubes (usually single-walled carbon nanotubes (SWCNTs) and multi-walled carbon nanotubes (MWCNTs)) have been intensively focused on academic researches and mass-produced for wide applications such as composite materials, biosensors, and drug delivery systems. However, due to oxidative stress-dependent and physically-induced cellular toxicity of CNTs, many efforts to render biocompatible and biodegradable properties in CNTs have been highlighted. Thus, taking into the consideration of exposure in human health and the environment, biodegradation of CNTs as a potential disposal is highly addressed. In this study, lignin peroxidase (LiP) was isolated and partially purified from the fruiting bodies of the edible mushroom Sparassis latifolia (S. latifolia). The biodegradation of raw grade and thermally-treated carboxylated SWCNTs (denoted as ASA and AST) with this enzyme was investigated, prior to more biodegradation-resistant MWCNTs. The interactions between the SWCNTs and LiP were investigated using various techniques, and the intermediate byproducts of the LiP degradation were identified. Our findings demonstrated that both ASA and AST were efficiently degraded by LiP where the producing radicals by the LiP played a critical role in the biodegradation of SWCNTs. The final degraded products were confirmed with the generation of CO2 gas. Conclusively, the low extraction cost of partially purified enzyme from mushrooms can make this approach a promising alternative in environmental bioremediation as a practical application.

Oxidative biodegradation of single-walled carbon nanotubes by partially purified lignin peroxidase from <i>Sparassis latifolia</i> mushroom

Chandrasekaran, Gayathri,Choi, Soo-Kyung,Lee, Young-Chul,Kim, Geun-Joong,Shin, Hyun-Jae Elsevier 2014 Journal of industrial and engineering chemistry Vol.20 No.5

<P><B>Abstract</B></P> <P>Two types of carbon nanotubes (usually single-walled carbon nanotubes (SWCNTs) and multi-walled carbon nanotubes (MWCNTs)) have been intensively focused on academic researches and mass-produced for wide applications such as composite materials, biosensors, and drug delivery systems. However, due to oxidative stress-dependent and physically-induced cellular toxicity of CNTs, many efforts to render biocompatible and biodegradable properties in CNTs have been highlighted. Thus, taking into the consideration of exposure in human health and the environment, biodegradation of CNTs as a potential disposal is highly addressed. In this study, lignin peroxidase (LiP) was isolated and partially purified from the fruiting bodies of the edible mushroom <I>Sparassis latifolia</I> (<I>S. latifolia</I>). The biodegradation of raw grade and thermally-treated carboxylated SWCNTs (denoted as ASA and AST) with this enzyme was investigated, prior to more biodegradation-resistant MWCNTs. The interactions between the SWCNTs and LiP were investigated using various techniques, and the intermediate by-products of the LiP degradation were identified. Our findings demonstrated that both ASA and AST were efficiently degraded by LiP where the producing radicals by the LiP played a critical role in the biodegradation of SWCNTs. The final degraded products were confirmed with the generation of CO<SUB>2</SUB> gas. Conclusively, the low extraction cost of partially purified enzyme from mushrooms can make this approach a promising alternative in environmental bioremediation as a practical application.</P>

충진재의 생분해도가 돈분 퇴비화 효율에 미치는 영향에 관한 연구

김성균,최경호,정문식 한국환경보건학회 1997 한국환경보건학회지 Vol.23 No.2

A study on the effect of the biodegradability of the composting bulking agent in the swine manurecomposting was carried out in a batch system. The purpose of this study is to prove the effect of the biodegradability of the composting-bulking agent on the efficiency of the composting. In this study, it is the lignins: Klason-Lignin in the volatile solid that the index of the biodegradability of the composting-material mixes which are pig manure-rice straw pig manure-sawdusts pig manuremixture of rice and ricestraw (2:1) pig manure-mixture of rice and sawdust (1:1). It was carried out in the same condition (moisture contents, air supply rate, C/N ratio, initial input weight, porosity-structure) except the biodegradability of the raw material mixes. One of the results from this study is that the biodegradability of the bulking agent in the sense of the VS lignin content is not an insignificant factor in composting reaction. The less contents of the lignin in VS, the more efficiencies of the cornposting reaction in use of these parameters for the degree of the reaction: temperature, the trends of the ash contents, the change pattern of the C/N ratio. Under some assumptions, it is able to induce rough model on the relation of the VS lignin contents with the efficiency of the degradability. In this model, the biodegradability of the bulking agent is not an insignificant factor however, it is flexible within some degrees of range.

Construction and Characterization of Fully Biodegradable Foam from Lignin-reinforced PBAT Biocomposites

홍서화,박진환,황석호 한국공업화학회 2021 한국공업화학회 연구논문 초록집 Vol.2021 No.1

미동정 부후균에 의한 소나무재의 Lignin 분해와 주사전자현미경 (SEM)을 이용한 관찰

박헌 ( Heon Park ),민경희 ( Kyeong Heui Min ) 한국목재공학회 2003 목재공학 Vol.31 No.4

Discovery of Hypothetical Proteins of Phanerochaete chrysosporium Secretome as Accessory Proteins of Lignin Peroxidase for Lignin Biodegradation

Seung-hyun HAN,Yong Hwan KIM 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10

The white rot fungus Phanerochaete chrysosporium is one of the well-known lignin biodegrading organisms with secretory enzymes lignin peroxidase (LiP) and manganese peroxidase. Lignin utilization using living organisms is less efficient because cultivating fungi or harvesting degradation products are not easy. Therefore in vitro lignin degradation with native or recombinant LiP from common expression hosts like Escherichia coli or Pichia pastoris has been investigated. However, there are also some challenges, for example, low enzyme yieldsin native system, or insoluble expression in recombinant system. Another big problem in in vitro lignin biodegradation is that the degradation products are not successfully obtained yet. Therefore, it is estimated that there are fundamental differences between in vitro and Mother Nature conditions for lignin degradation. In this study, we hypothesized that there will be accessory proteins in the extracellular space of P. chrysosporium that co-work with LiP for lignin degradation. We searched all reported hypothetical proteins of the secretome of P. chrysosporium and attempted to express these proteins to find any synergistic effects with LiP.

Plant Terpenes and Lignin as Natural Cosubstrates in Biodegradation of Polyclorinated Biphenyls(PCBs)and Polycyclic Aromatic Hydrocarbons(PAHs)

Koh, Sung-Cheol,Park, Young-In,Koo, Yoon-Mo,So, Jae-Seong 한국해양대학교 해양과학기술연구소 2000 硏究論文集 Vol.9 No.2

The objective of this minireview is to examine how cometabolic biodegradation of polychlorinated biphenyls (PCBs) and polycyclic aromatic hydrocarbons (PAHs) might be affected by plant terpenes and lignins as natural substrates abundant in nature. The topics covered, hence, are environmental significance of PCBs and PAHs, nature and distribution of plant terpenes and lignin, structural and metabolic similarities of the natural compounds to PCBs and PAHs, and possible roles of the natural substrates in inducing the biodegradative pathways of PCBs and PAHs.