마우스, 랫트, 해명에서 집먼지 진드기 추출물의 일반 약리시험과 급성 및 아급성 독성에 관한 연구

노재열,김경환 한국독성학회 1998 Toxicological Research Vol.14 No.2

It has been reported that 50~70% of child asthma, bronchial asthma in adult, and allergic rhinitis are caused by house dust mite. The antigen extracted from house dust mite has been used for effective treatment against allergic diseases and for clinical test. This house dust mite antigen has been entirely imported from abroad. However, the composition and content of all the antigen imported vary from a brand to other brand. Thus, we need to standardize the composition and content of the antigen by developing it domestically. We proceeded pre-clinically general pharmacological test and toxicological test that are required for the eventual human use by utilizing the house dust mite cultured in Korea. In order to obtain information on general pharmacological tests such as its toxic signs in tissues or organs which are mainly affected, we examined the effect of house dust mite on the tensions of the isolated tissues and heart rates of cardiac muscle by recording with force displacement transducer of polygragh (Glass Model 7). We determined lethality of antigen extracted from house dust mite in mice and guinea pigs. We examined acute and subacute toxicity by administrating house dust mite extract of 500, 100, 20 times of the expected clinical dose. In male and female mice and guinea pigs, given a sigle intraperitoneal dose of antigen, $LD_{50}$ values were over 5.0 $\textrm{m}{\ell}$/kg, respectively. In animals administrated with house dust mite, there were no significant change of clinical symptom, body weight, food consumption, water consumption, eye examinations, urinalysis, blood biochemistry, and histopathological examinations in any animals tested. We found no toxic effect of this house dust mite. These results show that the house dust mite cultured by us could be used in the development of medicine against allergic diseases caused by the antigen of house dust mite.

우리나라 집먼지진드기 생태에 관한 고찰 및 표준 조사법 제안

용태순,정경용 대한 소아알레르기 호흡기학회 2011 Allergy Asthma & Respiratory Disease Vol.21 No.1

A large number of house dust mites live in homes worldwide and produce the most important allergens which cause allergic diseases to the genetically predisposed individuals perennially. Herewith, the literatures published on the survey of dust mites in Korea were reviewed. In most studies, mites were isolated and investigated from the house dust samples collected by using house- hold vacuum cleaners. Among more than 30 species of house dust mites recorded in Korea, Dermatophagoides farinae was found to be the most predominant species in homes, followed by D. pteronyssinus. House dust mites were most frequently encountered in dust from bedding samples followed by kitchen samples, in which Tyrophagus putrescentiae, a storage mite species, was the most frequently found species. The highest mite density and allergen concentration were shown in autumn. It would be useful if the standard method for the investigation of dust mite density is established for the field survey. Measuring the number of mites per unit area (1 m^2) collected in a given time (2 minutes) using a house-hold vacuum cleaners equiped with nonwovens is suggested here as an objective and convenient method for the survey on house dust mites. Mites can be detected directly from fine dust samples using a stereomicroscope or isolated by technique utilizing saturated salt water. In the future, more field surveys and laboratory studies are necessary for the investigation on the relationship between house dust mites in homes and various allergic diseases.

일반 스폰지베개 및 깃털베개 커버의 집먼지와 집먼지진드기 투과성

남해선 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

Previous studies have demonstrated significantly higher house dust mite allergen levels on synthetic pillows, compared to feather pillows. Reasons for these differences could be lower permeability of feather pillow coverings to allergen, greater house dust mite penetration of synthetic pillow covering, or a combination of both. This study was done to determine the permeability of synthetic and feather pillow coverings to house dust mites and house dust. Twenty live adult house dust mites were seeded on top of a type of synthetic and a type of feather pillow coverings (all in dupicate) with adequate food supply below in sealed culture dished and kept at 23℃ and 70% relative humidity. After 24hr and 48hr house dust mite numbers remaining on top of the coverings were enumerated microscopically. Aliquots of fine house dust (in triplicate) were placed on top of the synthetic and feather pillow coverings, shaken gently for 30min and penetrated dust collected and weighed. After 24hr, all 20 house dust mites had penetrated the standard synthetic pillow coverings, while no house dust mites had penetrated either the feather pillow after 48hr. Dust permeability for the standard synthetic and feather pillow coverings were 0.88% and 0.07%, respectively. These findings of total permeability of standard synthetic pillow coverings to house dust mites, and their greater permeability to house dust could explain their reported higher house dust mite allergen levels, compared to feather pillow coverings.

우리나라 집먼지진드기 생태에 관한 고찰 및 표준 조사법 제안

용태순 ( Tai Soon Yong ),정경용 ( Kyoung Yong Jeong ) 대한소아알레르기호흡기학회(구 대한소아알레르기 및 호흡기학회) 2011 소아알레르기 및 호흡기학회지 Vol.21 No.1

A large number of house dust mites live in homes worldwide and produce the most important allergens which cause allergic diseases to the genetically predisposed individuals perennially. Herewith, the literatures published on the survey of dust mites in Korea were reviewed. In most studies, mites were isolated and investigated from the house dust samples collected by using household vacuum cleaners. Among more than 30 species of house dust mites recorded in Korea, Dermato-phagoides farinae was found to be the most predominant species in homes, followed by D. pteronyssinus. House dust mites were most frequently encountered in dust from bedding samples followed by kitchen samples, in which Tyrophagus putrescentiae, a storage mite species, was the most frequently found species. The highest mite density and allergen concentration were shown in autumn. It would be useful if the standard method for the investigation of dust mite density is established for the field survey. Measuring the number of mites per unit area (1 m2) collected in a given time (2 minutes) using a house-hold vacuum cleaners equiped with nonwovens is suggested here as an objective and convenient method for the survey on house dust mites. Mites can be detected directly from fine dust samples using a stereomicroscope or isolated by technique utilizing saturated salt water. In the future, more field surveys and laboratory studies are necessary for the investigation on the relationship between house dust mites in homes and various allergic diseases. [Pediatr Allergy Respir Dis(Korea) 2011;21:4-16]

일부 주택의 침구류 및 실내공기 중의 집먼지 진드기 알러젠 농도 측정

손종렬(Jong-Ryeul Sohn),최달웅(Dal-Woong Choi),백용규(Yong-Kyu Baik) 한국생활환경학회 2006 한국생활환경학회지 Vol.13 No.3

House dust mite were the most important cause of allergic asthma and rhinitis. More than 70% of Korean children and about 50% of adult with respiratory allergy were sensitive to house dust mite. This experiment was examined the indoor environment in house and house dust mite existence inquiry. From, 23rd December 2005 to 28th February 2006, dust samples were collected from the bedclothing by a vacuum cleaner and air sampler. And the levels of Der f1 and Der p1 were determined by ELISA (Enzyme-Linked Immunosorbent Assay). The results were as follows : 1. The mite allergy contaminations of bedclothes in house were higher than international standards (2000 ng/g), 2. In type of mite, The Korean house the almost have the D. farinae other than D. pteronyeeinus of mite. 3. The Der f1 and Der p1 levels per gram of dust from the bedclothes were 2074.99 ng on average, but they did not exist in air. The concentrations of house dust mite were significantly high in the bedclothing. This results suggest that the bedclothes have enough concentrations of dust mites to develop the sensitization. The control of indoor environment should be emphasized to prevent the sensitization by the repeated exposure to dust mite.

일부 실내 환경 시설의 집먼지 진드기 검출에 따른 PM10과 HCHO의 비교평가

손종렬(Jong-Ryeul Sohn),강민구(Min-Gu Kang),곽호찬(Ho-Chan Kwak) 한국실내환경학회 2008 한국실내환경학회지 Vol.5 No.1

House dust mite of the indoor environment is one of the most important causes of allergic asthma and rhinitis. It has been known that more than 70% of Korean children and about 50% of adult with the respiratory allergy are sensitive to house dust mite. The purpose of our study is to find out the relationship between the concentration of formaldehyde (HCHO)/PM10 and the number of house dust mite in the indoor environment. In this study, sampling sites were education, social and dwelling facilities. We inquire the questionnaire of the resident on the indoor air quality in all sampling sites. At the same time, we measured the concentration of formaldeh yde(HCHO)/PM10 as well as house dust mites. it was complained by 20% respondents that the indoor air quality was bad and by 10% that the atopy symptom was evident. When a certain amount of house dust mites were detected, the contents of PM10 were observed high and that of HCHO low. However when the house dust mites were not detected, the concentration of PM10 was low and that of HCHO high. From our results, it is concluded that the indoor air quality (i.e. conc. of PM10/HCHO) is quite related to the contents of house dust mites supporting the fact that the symptom of atopy and asthma is due to these small organisms.

집 먼지진드기에 대한 유칼립투스정유의 기피효과

이재영 ( Jae Young Lee ),지차호 ( Cha Ho Jee ) 한국가축위생학회 2010 韓國家畜衛生學會誌 Vol.33 No.2

Majority of asthma and atopic dermatitis are known to be spontaneously sensitive to house dust mite allergen. Control of house dust mite populations has been principally achieved by using chemical insecticides. But the risk to human health would be a potential problem. Moreover, house dust mite remain as allergens even after death. So, It is more effective method keeping the house dust mites away than killing them. The use of plant-derived repellents has been considered as a promising alternative to chemical repellents. Eucalyptus is a diverse genus of flowering trees in the myrtle family, Myrtaceae. It has insect repellent properties and is an active ingredient in some commercial mosquito repellents. These studies were carried out to investigate repellent effects of Eucalyptus oil against house dust mites and compare lemongrass oil. Eucalyptus oil and lemongrass oil were exposed at different doses (0.2, 0.1, 0.05, 0.025, 0.0125, 0.00625μl/cm2) and different times (1, 3, 6 hours) on house dust mites. The most effective dose of Eucalyptus and lemongrass oils against house dust mites was 0.1μl/cm2 and 0.025μl/cm2. Each repellent effect (%) in most effective dose was 90.3% (Eucalyptus) and 80.8% (lemongrass).

진드기 체항원을 이용한 새응애 감염증에 대한 면역효과

이삼선,김재원,지차호,Lee, Sam-Sun,Kim, Jae-Won,Jee, Cha-Ho 대한수의학회 2002 大韓獸醫學會誌 Vol.42 No.2

Fowl red mite, Dermanyssus gallinae, is the most important ectoparasite affecting egg layers worldwide. More than 35 compounds have been used for fowl red mite control. Although some of them are efficient, several compounds are unsuitable in terms of food safety and environmental problems. Some compounds are efficient in theory but inadequate in practice. It is also expensive in material and labor to control effectively. Effective doses are very close to toxic doses and repeated treatment is required. Repeated, long term treatment of compounds on fowl red mite populations, may cause heritable resistance against the mites. In this study, antigenicity of fowl red mite and house dust mite, Dermatophagoides pteronyssinus, were identified by SDS-PAGE, silver staining, Western blotting and ELISA to investigate immune effects against fowl red mite using somatic antigens of fowl red mite and house dust mite. By SDS-PAGE, silver staining and Western blotting, several common antigens (110, 60, 56, 49, 46 kDa) of both fowl red mite and house dust mite were recognized. To identify immune effect of somatic antigens of fowl red mite and house dust mite, sixty white leghorn broilers(1 week old) were used. Among sixty white leghorn broilers, twenty were immunized with fowl red mite somatic antigens(Group I), twenty immunized with house dust mite antigens(Group II), and twenty were control group without antigen(Group III), respectively. After immunization, it was identified that antibody titers were increased both in group and II. Then all groups were challenged with fowl red mites. After 2 months, measurements of body weights, packed cell volume(PCV), ELISA OD values and numbers of mites were significant(p<0.05). These results suggest that fowl red mite and house dust mite, which are easy to collect and maintain, can be good vaccine candidates against fowl red mite in chicken.

집먼지진드기 체항원을 이용한 개 옴 감염증에 대한 면역효과

윤인수,김재원,지차호,Yoon, In-Soo,Kim, Jae-Won,Jee, Cha-Ho 대한수의학회 2003 大韓獸醫學會誌 Vol.43 No.4

Canine sarcoptic mite (Sarcoptes scabiei var. canis) burrow usually in the stratum corneum of the skin of dogs and rabbits. Antigens from the burrowing mites induce cutaneous inflammatory reaction and humoral and cell-mediated immune response in the host. The effect of immunization induced by somatic antigens of house dust mite (Dermatophagoides spp.) has been evaluated to control the canine sarcoptic mite in this experiment. Twelve common antigens (187, 142, 126, 120, 109, 92, 80, 68, 51, 30, 25, 17 kDa) were found using SDS-PAGE with silver staining and Western blot between canine sarcoptic mite and house dust mite. In order to evaluate the immunologic effect of these common antigens 10 New Zealand white rabbits were divided as 4 groups such as negative control (group I), positive challenged control (group II), vaccinated (group III), and vaccinated-challenged (group IV) groups. Group II was artificially infested with about 1,000 canine sarcoptic mites and group III and IV were immunized with somatic antigens of house dust mite. In addition group IV was artificially infested with about 1,000 canine sarcoptic mites and group II, IV were treated with ivermectin. At the 8 weeks of the vaccination with common antigen, the antibody titers of all groups of II, III and IV had been increased. Both infestation score and live canine sarcoptic mite counts of group IV were lower than group III. Infestation score of group II become 0 by 2 weeks and group IV by 4 weeks after infestation. These results suggest that house dust mite, which is easy to culture in vitro, can be a vaccine candidate for protection of canine sarcoptic mite infestation.

집 먼지 진드기에 대한 유칼립투스 세제의 기피효과

김혜성 ( Hye Sung Kim ),지차호 ( Cha Ho Jee ) 한국동물위생학회 2015 韓國家畜衛生學會誌 Vol.38 No.2

The exposure to house dust mites is closely connected with allergic diseases such as atopic dermatitis, allergic rhinitis and asthma. Control of house dust mite populations has been principally achieved by using chemical insecticides. But the risk to human health would be a potential problem. Moreover, house dust mite remain as allergens even after death. Therefore, repelling the house dust mites is more effective method than killing them. The use of plant-derived repellents has been considered as a promising alternative to chemical repellents. Eucalyptus oils are regarded as safe compounds, there have been attempts to commercialize and market the insecticides/repellent products containing Eucalyptus oil as such or based upon them. For that reason, we experimented repellent activity against these mites using laundry detergent with Eucalyptus oil in this experiment. House dust mites (Dermatophagoides farinae and Dermatophagoides pteronyssinus) were exposed at different concentrations (4, 8, 16, 32 L/mL) in fabric treated laundry detergent with Eucalyptus oil for 24 hours. The repellent effects (%) of laundry detergent with Eucalyptus oil exposed at 4, 8, 16, and 32 L/mL were 48.16%, 74.62%, 52.67%, and 44.47%, respectively. These results suggest that laundry detergent with Eucalyptus oil have potential effect to repel the house dust mites.