돼지 단독균 인공감염 및 항생제 치료에 따른 Haptoglobin치의 변화

문진산,남향미,구복경,주이석,정석찬,김종염,박용호,Moon, Jin-san,Nam, Hyang-mi,Ku, Bok-gyeong,Joo, Yi-seok,Jung, Suk-chan,Kim, Joang-yeom,Park, Yong-ho 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.3

The changes of concentration of serum haptoglobin, body temperature, hematological result and antibody titers were compared in pigs given intravenously injection of E rhusiopathiae and control pigs. Pigs administered with E rhusiopathiae injections showed acute septicemia and arthritis. Also, statistically significant changes observed in the total white cell counts and body temperature. An increased haptoglobin concentration in serum was detected after E rhusiopathiae infection, but not during antibiotics treatment. The results indicated that measurement of the concentration of serum haptoglobin may be useful in the early diagnosis of infectious disease, such as Erysipelas.

오제스키병에 감염된 돼지의 serum amyloid A와 haptoglobin의 농도 변화

오윤택 ( Yoon Taek Oh ),조정곤 ( Jeong Gon Cho ) 한국가축위생학회 2002 韓國家畜衛生學會誌 Vol.25 No.1

The acute phase serum protein response is a well-known general indicator of inflammation, trauma or other pathological conditions and its relevance for the monitoring of the health status of domestic animals is being increasingly realized. The changes in serum protein composition which occur after tissue damage represent a part of the systemic response of the injured animals which is mediated by pro-inflammatory cytokines such as TNF-a, IL-6 and IL-1. These responses play a vital role in containing the tissue damage and enhancing the processes of repair and resolution. From a clinical perspective, the assay of acute phase proteins can provide a method for detecting inflammation. In animals, the most sensitive acute phase proteins are haptoglogin, serum amyloid A and a1-acid glycoprotein in response to inflammatory condition. The aim of this study was to assess the diagnostic value of the concentrations of serum amyloid A(SAA) and haptoglobin(HP) in serum of pigs infected with Aujeszky`s disease virus(ADV). Fifty pigs infected with ADV and 5 normal pigs were used in this experiment. The mean serum concentration of SAA of pigs infected with ADV was 96.8±7.1㎍/㎖(range, 36.0~187.5㎍/㎖) and that of normal pigs was 42.9±3.3㎍/㎖(range, 17.3~127.8㎍/㎖). The mean serum concentration of HP of pigs infected with ADV was 1,164.4 ± 96.9㎍/㎖(range, 790.2~1,769.2㎍/㎖) and that of normal pigs was 675.4±56.3㎍/㎖(range, 650.0-690.4㎍/㎖). The mean concentrations of SAA and HP in serum of pigs infected with ADV compared with those of normal pigs showed approximately a two-fold. It was concluded that the concentrations of SAA and HP in serum may proved to be diagnostic marker of Aujeszky`s disease.

Utilization of Ferroproteins by Candida albicans during Candidastasis by Apotransferrin

한용문 대한약학회 2005 Archives of Pharmacal Research Vol.28 No.8

Many reports have stated that some of the pathogenic bacteria can obtain iron from ferroproteins, such as cytochrome C, ferritin, hemin, hemoglobin, and myoglobin. These reports prompted us to determine if an opportunistic pathogenic fungus, Candida albicans, can utilize ferroproteins to circumvent the iron-regulatory effect of transferrin. The following assays were carried out to measure in vitro growth stimulation by the ferroproteins: as an initial step, C. albicans was cultured in iron-free (pretreated with apotransferrin for 24 h) culture medium. Once Candida albicans yeast cell growth reached stasis from iron starvation, individual ferroproteins were added to the culture media. Results showed that hemin, hemoglobin, and myoglobin supported a partial growth recovery. Additional studies with haptoglobin, a serum protein that interacts with the globin moiety of certain ferroproteins, established that C. albicans could obtain iron from the haptoglobin-ferroprotein complexes. These data indicate that the heme part of the ferroproteins is the source of iron. This implies that heme oxygenase, CaHMX1 might be involved in bringing about dissociation of heme-containing protein for iron-acquisition. In addition, anticandidal activity of transferrin takes place not only by the process of iron regulation, but also by direct interaction with the yeast cells

High prevalence of renal salt wasting induced by haptoglobin-related protein without signal peptide is linked to new syndrome of salt wasting in Alzheimer disease

Maesaka John K.,Imbriano Louis J.,Grant Candace,Miyawaki Nobuyuki 대한신장학회 2024 Kidney Research and Clinical Practice Vol.43 No.1

The subject of hyponatremia is undergoing significant changes after developing a more pathophysiologic approach that is superior to the ineffective volume approach and can more effectively identify the different causes of hyponatremia. This new approach identified cerebral salt wasting (CSW) in 24 (38%) of 62 hyponatremic patients from the medical wards of the hospital with 21 showing no evidence of cerebral disease to support our proposal to change CSW to renal salt wasting (RSW). RSW had to be differentiated from the syndrome of inappropriate secretion of antidiuretic hormone (SIADH) because of diametrically opposite therapeutic goals of water-restricting water-logged patients with SIADH or administering salt water to volume-depleted patients with RSW. Both syndromes present with identical clinical parameters that require a difficult protocol to make such a differentiation possible. We describe rat clearance studies demonstrating natriuretic activity in the plasma of patients with neurosurgical and Alzheimer diseases (AD) and eventually identify the protein as haptoglobin-related protein without signal peptide, which can serve as a biomarker to simplify diagnosis of RSW and delivery of the proper management to improve clinical outcomes. We also discuss the introduction of a new syndrome of RSW in AD and its implications. The high prevalence of RSW and identification of the natriuretic factor have created debates over the existence of RSW with none questioning or addressing the pathophysiologic data that identified patients with RSW. We also discuss the potentially large group of patients with RSW who are normonatremic.

Utilization of Ferroproteins by Candida albicans during Candidastasis by Apotransferrin

Han, Yong-Moon The Pharmaceutical Society of Korea 2005 Archives of Pharmacal Research Vol.28 No.8

Many reports have stated that some of the pathogenic bacteria can obtain iron from ferroproteins, such as cytochrome C, ferritin, hemin, hemoglobin, and myoglobin. These reports prompted us to determine if an opportunistic pathogenic fungus, Candida albicans, can utilize ferroproteins to circumvent the iron-regulatory effect of transferrin. The following assays were carried out to measure in vitro growth stimulation by the ferroproteins: as an initial step, C. albicans was cultured in iron-free (pretreated with apotransferrin for 24h) culture medium. Once Candida albicans yeast cell growth reached stasis from iron starvation, individual ferroproteins were added to the culture media. Results showed that hemin, hemoglobin, and myoglobin supported a partial growth recovery. Additional studies with haptoglobin, a serum protein that interacts with the globin moiety of certain ferroproteins, established that C. albicans could obtain iron from the haptoglobin-ferroprotein complexes. These data indicate that the heme part of the ferroproteins is the source of iron. This implies that heme oxygenase, CaHMX1 might be involved in bringing about dissociation of heme-containing protein for iron-acquisition. In addition, anticandidal activity of transferrin takes place not only by the process of iron regulation, but also by direct interaction with the yeast cells.

Anaphylactic Transfusion Reaction in a Patient with Anhaptoglobinemia: The First Case in Korea

Hyunsoo Kim,Jonghyeon Choi,박경운,김현숙,민유홍,Moon Jung Kim,김현옥 대한진단검사의학회 2012 Annals of Laboratory Medicine Vol.32 No.4

Anaphylactic transfusion reactions are rare complications of blood transfusions. Anhaptoglobinemia, a condition that has high incidence in Asia, can cause allergic transfusion reactions or anaphylaxis in severe cases. A 50-yr-old Korean woman was diagnosed with relapsed acute promyelocytic leukemia. She developed thrombocytopenia during chemotherapy and an anaphylactic transfusion reaction on the 4th and 5th platelet transfusions immediately after the transfusion of the platelet concentrates was initiated. Blood analysis showed no detectable serum haptoglobin. We examined her genetic phenotype and detected anhaptoglobinemia, which occurs because of an allelic deletion in the Hp gene cluster. The presence of an antibody against haptoglobin was detected by performing ELISA. To prevent anaphylactic reactions, apheresis platelets were transfused after washing. Consequently, anaphylactic transfusion reactions did not develop. Here, we report the first case of anhaptoglobinemia causing anaphylactic transfusion reaction in Korea.

Evidence of Tandem Repeat and Extra Thiol-groups Resulted in the Polymeric Formation of Bovine Haptoglobin: A Unique Structure of Hp 2-2 Phenotype

( Yi An Lai ),( I Hsiang Lai ),( Chi Feng Tseng ),( James Lee ),( Simon J. T. Mao ) 생화학분자생물학회 2007 BMB Reports Vol.40 No.6

Utilization of ferroproteins by Candida albicans during candidastasis by apo-ovotransferrin

한용문 동덕여자대학교 종합약학연구소 2001 동덕약학연구지 Vol.5 No.-

Recent reports have shown that some pathogenic bacteria can obtain iron from ferroproteins, such as cytochrome C, ferritin, hemin, hemoglobin and myoglobin. These studies prompted to determine if the opportunistic pathogenic fungi, Candida albicans, can utilize ferroproteins to circumvent the iron-regulatory effect of ovotransferrin. Assays to measure growth stimulation in vitro by the ferroproteins were conducted as follows: C. albicans was cultured in iron-free (pretreated with apo-ovotransferrin for 24 h) culture medium. Once C. albicans yeast cell growth reached stasis from iron starvation, individual ferroproteins were added to the culture media. The results suggested that hemin, hemoglobin, and myoglobin were able to support a partial growth recovery. Additional studies with haptoglobin, a serum protein that interacts with the globin moiety of certain ferroproteins, established that C. albicans was able to obtain iron from the haptoglobin-ferroprotein complexes. This result suggests that the heme group may be the iron source from the ferroproteins.

Evidence of Tandem Repeat and Extra Thiol-groups Resulted in the Polymeric Formation of Bovine Haptoglobin: A Unique Structure of Hp 2-2 Phenotype

Lai, Yi An,Lai, I Hsiang,Tseng, Chi Feng,Lee, James,Mao, Simon J.T. Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.6

Human plasma Hp is classified as 1-1, 2-1, and 2-2. They are inherited from two alleles Hp 1 and Hp 2, but there is only Hp 1 in almost all the animal species. Hp 2-2 molecule is extremely large and heterogeneous associated with the development of inflammatory-related diseases. In this study, we expressed entire bovine Hp in E. coli as a $\alpha\beta$ linear form. Interestingly, the antibodies prepared against this form could recognize the subunit of native Hp. In stead of a complicated column method, the antibody was able to isolate bovine Hp via immunoaffinity and gelfiltration columns. The isolated Hp is polymeric containing two major molecular forms (660 and 730 kDa). Their size and hemoglobin binding complex are significantly larger than that of human Hp 2-2. The amino-acid sequence deducted from the nucleotide sequence is similar to human Hp 2 containing a tandem repeat over the $\alpha$ chain. Thus, the Hp 2 allele is not unique in human. We also found that there is one additional -SH group (Cys-97) in bovine $\alpha$ chain with a total of 8 -SH groups, which may be responsible for the overall polymeric structure that is markedly different from human Hp 2-2. The significance of the finding and its relationship to structural evolution are also discussed.

Haptoglobin Concentration in the Cord Blood of Uninfected Korean Newborns

( Seong Jin Choi ),( Byoungkook Lee ),( Kwangjin Ahn ),( Young Uh ) 대한주산의학회 2018 Perinatology Vol.29 No.4

Objective: Highly sensitive haptoglobin measurement should be used in neonates because the haptoglobin concentration in neonates is lower than that of adults. The aim of this study was to establish the reference values of haptoglobin levels in the cord blood of uninfected neonates. Methods: The cord blood of 29 preterm and 51 term babies was collected, and data from the mother and the newborn were recorded. The haptoglobin concentrations of 80 cord blood samples were simultaneously measured by enzyme-linked immunosorbent assay (ELISA; Assaypro, St Charles, MO, USA) and immunoturbidimetry assay (Roche Diagnostics, Basel, Switzerland). C-reactive protein (CRP) was also measured by immunoturbidimetry assay (Roche Diagnostics, Switzerland). Results: Mean values of CRP and ELISA haptoglobin were not significantly different between preterm and term babies. The 2.5 percentile and 97.5 percentile values of ELISA haptoglobin concentration were as follows: 80 neonates, 0.01 mg/dL and 0.59 mg/dL; 29 preterm babies, 0.08 mg/dL and 0.18 mg/dL; and 51 term babies, 0.07 mg/dL and 0.23 mg/dL. There were no differences in ELISA haptoglobin concentration according to maternal underlying diseases, delivery method, usage of antibiotics or steroids before delivery, gestational age, gender of baby, or twin gestation. Conclusion: A highly sensitive haptoglobin method should be used to determine the haptoglobin concentration in Korean newborns because the reference values of cord blood haptoglobin concentration in Korean newborns are less than the lower detection limit for commonly used immunoturbidimetric haptoglobin measurement methods.