전해산화수의 제어 조건에 따른 살균 효과

정기정 ( Ki Jeong Jeong ),박수길 ( Soo Gil Park ) 충북대학교 건설기술연구소 2012 建設技術論文集 Vol.31 No.2

Electrolyzed water generated by electrolysis of added sodium chloride in distilled water Of Lap water. This electrolyzed water was separated Electrolyzed Reduced Water[ERW] and Electrolyzed Acidic Water[EAW]. Electrolyzed Acidic Water has some characteristics that is pH 2.2 ~ 2.6, Oxidation-Reduction Potential(ORP) > +1100mV, Available Chorine Concentration(ACC) 20 ~ (j(Jppm Electrolyzed Reduced Water had some characteristics that is pH > 10, Oxidation-Reduction Potential(ORP) < -800mV, Available Chlorine Concentration(ACC) < 1ppm Electrolyzed Acidic Water can be easily manufactured and has low pH but, this water is safe and environmentally-friendly and shows strong sterilization effect. In this study, we confirmed sterilization effect of electrolyzed oxidation water. And we confirmed sterilization effect 311d principles of HOCl and ORP that main factors of sterilization effect. .Also, Vole confirmed how can applied in practical life. Resides, we experimel1Led sterilization effect using E.coli and Staphylococcus Aureus that is easily exposed in the practical life. In addition, we used various condition 3lld concentrations solutions(Electrolyzed Acidic Water and Distilled Water) to confirmed sterilization effect. Finally, we confirmed the result using method of Colony Count.

산성 전해수가 콩나물의 생육에 미치는 영향

윤동준,이정동,강동진,박순기,황영현 한국생명과학회 2004 생명과학회지 Vol.14 No.5

To investigate the effect of the electrolyzed acidic water for soybean sprouts growth, the responses of characteristics of soybean sprouts were evaluated. Soybean sprouts grown by the electrolyzed acidic water showed shorter length in total body, root, and hypocotyl, etc. but they were evaluated to be increased in hypocotyl diameter and weight per sprout. Total length of soybean sprouts grown for 5 days by electrolyzed acidic water were much shorter than those by tap water. Soybean sprouts grown by tap water showed rapid growth in length even after 5 days but no more growth in length for those grown by electrolyzed acidic water. The growth of hypocotyl showed the same tendency as total length. No difference in root length among the soybean sprouts grown for 4 ~ 11 days by electrolyzed acidic water while those grown by tap water showed continuous rapid growth in length. The diameter of hypocotyl was thicker in those grown by electrolyzed acidic water than those grown by tap water and increased up 5 days. The weight of cotyledon grown by electrolyzed acidic water showed the proportional increase to the growing days but those grown by tap water showed no increase in hypocotyl weight up to 7 days, but a little bit increase after 11 days with the growth of new buds. The fresh weight per sprout was higher in those grown by electrolyzed acidic water until 7 days than tap water but it was the same weight in 11 days cultivation. The electrolyzed acidic water effected on shortening of hypocotyl and root length, thickening of hypocotyl diameter, and enlarging of cotyledon during soybean sprout cultivation. 산성 전해수를 이용하여 콩나물을 생육시켰을 때 콩나물 관련 주요형질들의 반응을 조사하여 콩나물 재배수로 사용 가능성을 검토하였다. 산성전해수로 생육시킨 콩나물은 전체길이,뿌리길이, 하배축 길이 등이 짧아졌고, 하배축의 두께, 콩나물 개체당 무게 등은 늘어 난 것으로 평가되었다. 하배축 횡단면 관찰에서 산성전해수에서 자란 콩나물의 하배축내 세포가 대조구에 비해 커져 하배축의 두께가 두꺼워진 것으로 평가되었으며, 생육일수별 콩나물 주요 특성에서 콩나물 전체길이는 산성전해수에서 자란 콩나물의 전체 길이가 더 짧았으며, 수돗물에서 자란 콩나물은 생육 후 5일째 이후에 급격히 길이 신장을 하였으나 전해수에서 자란 콩나물은 길이 신장은 거의 변화가 없었다. 하배축의 신장은 콩나물 전체길이와 같은 경향을 보였는데 생육 후 5일째에는 품종간 신장의 차이가 관찰되었다. 뿌리 생육 특성은 전해수에서 생육시킨 콩나물은 생육 4일째에서 11일째까지 뿌리 길이의 차이가 없었으나 수돗물에서 생육시킨 콩나물의 뿌리길이는 생육일수가 증가함에 따라 급격하게 신장을 보였다. 하배축의 두께는 산성전해수에서 생육시킨 콩나물이 수돗물에서 생육시킨 콩나물보다 더 두꺼웠는데 생육 후 5일째까지 두께가 증가하다가 생육 후 5일째 이후부터 다소 감소하였으며, 콩나물 자엽의 무게는 산성전해수에서 생육시킨 콩나물의 자엽무게가 생육일수가 증가할수록 현저하게 증가하였으나 수돗물에서 생육시킨 콩나물은 생육 후 7일째까지는 거의 변화를 보이지 않았다. 콩나물의 생체중은 전해수에서 생육시킨 콩나물이 재배 후 7일 까지는 무거웠으나 11일째는 산성전해수와 수돗물에서 자른 콩나물의 생체중이 거의 비슷하였다.

해조류를 이용한 해수소금 제조기법 및 성분분석

이승원,김현주,문덕수,정동호,최학선 한국해양공학회 2007 韓國海洋工學會誌 Vol.21 No.4

In this research, we have developed a manufacturing process for seawater salt by horizontal spray drying technique using the deep ocean water and seaweed(sea tangle). Deep ocean water, strong acidic electrolyzed water and strong alkaline electrolyzed water were used as extraction solvent of seaweed. Sodium content in seaweed extract solution by strong alkaline electrolyzed water was 1.63 (mg/g), which was 3.5 times lower than of seaweed extract by strong acidic electrolyzed water. Major mineral content(Na, K, Ca) in seawater salt by deep ocean water were higher than strong acidic electrolyzed water and strong alkaline electrolyzed water. On the contrary, Mg contents in seawater salt by deep ocean water were lower than strong acidic electrolyzed water and strong alkaline electrolyzed water. Based on the results of seawater salt production using seaweed, it is possible to make low-salt efficiently.1. 서 론소금은 인간이 생존하기 위해 없어서는 안 될 필수적인 자원이며, 이를 구하기 위한 노력은 아주 오래 전부터 이루어져 왔으며 지금도 계속되고 있다. 21세기에 들어서 생활수준의 향상과 함께 성인병, 퇴행성질환, 대사성질환 등이 증가함에 발맞추어 약물에 의한 치료 못지않게 질병의 예방 및 치료 효능을 갖는 기능성 식품에 대한 필요성이 증가하고, 건강을 우선시 하는 경향이 강해지면서 좋은 소금에 대한 욕구가 강해지고 있다. 현재, 소금은 다양한 방법으로 제조되고 있으며, 식용 소금은 KS규격에 따라 천일염과 정제염으로 구분되고, 정제염은 다시 기계적으로 대량생산되는 기계염과 가열공정을 거친 가공염(구운소금, 볶은소금, 생금, 죽염)으로 구분되며, 외국에서 수입된 암염 등이 있다(문덕수 등, 2006). 소금의 제조와 그 특성에 관한 연구는 극히 미미하여 돌소금의 생산구조(Jeong, 1998)와 정제염에 관한 연구, 소금종류별 무기물 조성(Jo and Shin, 1998), 중금속 함량(Park et al., 2000; Hwang, 1988), 외형구조에 대한 부분적인 연구(Ha and Park, 1998) 등이 있을 뿐이다.

Clinical application of disinfectant water and alkaline reduced water in Tikapur hospital, Nepal

Ma Easter Joy Sajo,Yang-Suk Yoon,Kyu-Jae Lee,Dong-Won Ahn,Narendra Kumar Khanal 한국물학회 2013 한국물학회 학술대회 Vol.2013 No.03

Electrolyzed disinfectant water and alkaline reduced water have been utilized in different clinical treatment and application in different countries such as Japan, Korea, America and China. Electrolyzed disinfectant water is known for its strong antimicrobial effect and has been used as an effective disinfectant for medical supplies, hand washing, and for treatment of wounds, burns, diabetic foot, and other skin diseases. We aimed to improve health services in Tikapur including health services at Tikapur Hospital. We have clinically applied Electrolyzed Acidic Water in treatment for burn, diabetic foot, and wounds dressing in patients in Tikapur Hospital from August 2012 up to present. Wound and Burn dressing for patients using electrolyzed disinfectant water gave promising treatment effect, also, with the other patients treated with electrolyzed acidic water. Another application is the use of Alkaline Reduced Water (ARW) for patients with digestive disorders and stone diseases. Patients with kidney stones were administered with ARW with around 2L per day. Recently, improvements have believed to be found with patients in Tikapur hospital. The application of electrolyzed disinfectant water and alkaline reduced water suggests a progress and development in Tikapur hospital and also for improvement of health services to the community as well.

Comparison of Antimicrobial Activity of Electrolyzed Water Using Various Electrodes against Biofilm of Oral Pathogens

Yun S Yoo,Hyun-Seung Shin,Sung-Hoon Lee 대한구강생물학회 2015 International Journal of Oral Biology Vol.40 No.3

Biofilms of oral microbes can cause various diseases in the oral cavity, such as dental caries, periodontitis and mucosal disease. Electrolyzed water generated by an electric current passed via water using a metal electrode has an antimicrobial effect on pathogenic bacteria which cause food poisoning. This study investigated the antimicrobial activity of electrolyzed waters using various metal electrodes on the floatage and biofilms of oral microbes. The electrolyzed water was generated by passing electric current using copper, silver and platinum electrodes. The electrolyzed water has a neutral pH. Streptococcus mutans, Porphyromonas gingivalis and Tannerella forsythia were cultured, and were used to form a biofilm using specific media. The floatage and biofilm of the microbes were then treated with the electrolyzed water. The electrolyzed water using platinum electrode (EWP) exhibited strong antimicrobial activity against the floatage and biofilm of the oral microbes. However, the electrolyzed water using copper and silver electrodes had no effect. The EWP disrupted the biofilm of oral microbes, except the S. mutans biofilm. Comparing the different electrolyzed waters that we created the platinum electrode generated water may be an ideal candidate for prevention of dental caries and periodontitis.

산성전리수의 생물학적 특성

김윤경 ( Yoon Kyoung Kim ),민병술 ( Byoung Sul Min ),민중기 ( Joong Kee Min ),이종권 ( Jong Kwon Lee ),이윤배 ( Yoon Bae Lee ),류근걸 ( Kun Kul Ryoo ),이미영 ( Mi Young Lee ) 한국환경생물학회 2004 환경생물 : 환경생물학회지 Vol.22 No.2

본 연구에서는 산성전리수의 일반적인 생물학적 특성을 간략히 살펴보았다. 직선형 DNA를 산성전리수에서 4℃와 25℃에서 약 10분간 반응시킨 결과 각각 40%와 50%의 DNA가 분해되었다. 그러나 산성전리수를 사용한 고온에서의 DNA 증폭반응 실험에서 DNA 분해없이 정상적으로 DNA 증폭반응이 일어났다. 산성전리수가 단백질의 안정도에 미치는 영향을 살펴본 결과 증류수에서는 총 7일 동안의 반응시간동안 단백질의 분해가 거의 일어나지 않았으나, 산성전리수에서는 제4일에서부터 단백질의 분해가 본격적으로 일어나기 시작하였다. 산성전리수에서 볍씨를 발아시켜 본 결과 증류수에서와 동일한 발아율을 나타냈으며, 산성전리수는 배양토에서 벼 유묘의 뿌리의 길이와 총 길이를 억제시킨다. 산성전리수는 해양미세조류의 성장곡선과 세포수에는 거의 영향을 미치지 않았다. 또한 산성전리수는 Polyphenol oxidase의 비활성을 약 50% 억제시킴으로써 감자의 갈변을 억제하였다. Biological characteristics of anodic electrolyzed water were investigated in this study. Linear DNAs which were incubated at 4? and 25? for 10 mins in the anodic electrolyzed water were degraded about 40% and 50%, respectively. But the DNA was amplified pretty well without any degradation through polymerase chain reaction in the presence of anodic electrolyzed water. Protein degradation hardly occurred in the distilled water during entire incubation time of 7 days, while protein began to be degraded from 4 days in the anodic electrolyzed water. Rice seeds could germinate in the distilled water and anodic electrolyzed water with the same germination ratio, however, the anodic electrolyzed water inhibited the growth of roots and total length of rice seedlings in the soil. Anodic electrolyzed water did not affect the growth curve and cell number of marine alga significantly. The anodic electrolyzed water inhibited the browning of potato by inactivating 50% of polyphenol oxidase activity.

Antibacterial Effect of Electrolyzed Water on Oral Bacteria

Lee Sung-Hoon,Choi Bong-Kyu The Microbiological Society of Korea 2006 The journal of microbiology Vol.44 No.4

This study investigated the antibacterial effect of electrolyzed water on oral bacteria both in vitro and in vivo. Tap water was electrolyzed in a water vessel using platinum cell technology. The electrolyzed tap water (called Puri-water) was put in contact with five major periodontopathogens or toothbrushes contaminated with these bacteria for 30 sec. In addition, Puri-water was used as a mouthwash for 30 sec in 16 subjects and the antibacterial effect on salivary bacteria was evaluated. Puri-water significantly reduced the growth of all periodontopathogens in culture and on toothbrushes, and that of aerobic and anaerobic bacteria in saliva, when compared to the effect of tap water. It also significantly reduced mutans streptococci growing on mitis salivarius-bacitracin agar. Our results demonstrate that the electrolyzed tap water is effective as a mouthwash and for toothbrush disinfection.

Antifungal effect of electrolyzed hydrogen water on Candida albicans biofilm

표경열,유연승,백동헌 대한턱관절교합학회 2015 구강회복응용과학지 Vol.31 No.3

Purpose: Candida albicans can cause mucosal disease in many vulnerable patients. Also they are associated with denture-related stomatitis. Electrolyzed water is generated by electric current passed via water using various metal electrodes and has antimicrobial activity. The aim of this study was to investigate antifungal activity of electrolyzed water on C. albicans biofilm. Materials and Methods: C. albicans was cultured by sabouraud dextrose broth and F-12 nutrient medium in aerobic and 5% CO2 condition to form blastoconidia (yeast) and hyphae type, respectively. For formation of C. albicans biofilm, C. albicans was cultivated on rough surface 6-well plate by using F-12 nutrient medium in CO2 incubator for 48 hr. After electrolyzing tap water using various metal electrodes, the blastoconidia and hyphal type of C. albicans were treated with electrolyzed water. C. albicans formed blastoconidia and hyphae type when they were cultured by sabouraud dextrose broth and F-12 nutrient medium, respectively. Results: The electrolyzed water using palladium electrode (EWP) exhibited antifungal effect on blastoconidia of C. albicans. Also, the EWP significantly has antifungal activity against C. albicans biofilm and hyphae. In the electrolyzed water using various metal electrodes, only the EWP have antifungal activity. Conclusion: The EWP may use a gargle solution and a soaking solution for prevention of oral candidiasis and denture-related stomatitis due to antifungal activity.

Antibacterial Effect of Electrolyzed Water on Oral Bacteria

이성훈,최봉규 한국미생물학회 2006 The journal of microbiology Vol.44 No.4

This study investigated the antibacterial effect of electrolyzed water on oral bacteria both in vitro and in vivo. Tap water was electrolyzed in a water vessel using platinum cell technology. The electrolyzed tap water (called Puri-water) was put in contact with five major periodontopathogens or toothbrushes contaminated with these bacteria for 30 sec. In addition, Puri-water was used as a mouthwash for 30 sec in 16 subjects and the antibacterial effect on salivary bacteria was evaluated. Puri-water significantly reduced the growth of all periodontopathogens in culture and on toothbrushes, and that of aerobic and anaerobic bacteria in saliva, when compared to the effect of tap water. It also significantly reduced mutans streptococci growing on mitis salivarius-bacitracin agar. Our results demonstrate that the electrolyzed tap water is effective as a mouthwash and for toothbrush disinfection.

전기영동 겔과 녹차성분에 대한 환원전리수의 침투력과 용해력

류근걸(Ryoo Kun-kul),이윤배(Lee Yoon-Bae),이종권(Lee Jong-kwon),이미영(Lee Mi-Young) 한국산학기술학회 2005 한국산학기술학회논문지 Vol.6 No.1

본 연구에서는 전기영동 젤에 대한 환원전리수의 침투력과 녹차성분에 대한 환원전리수의 용해력을 일반 물과 서로 비교하였다. 환원전리수로 제조한 CBB-R 염색시약으로 polyacrylamide 젤 상에서 단백질을 다양한 시간 동안 염색한 후, 증류수로 제조한 CBB-R 염색시약에 의한 영색강도와 서로 비교하였다. 그 결과 환원전리수로 제조한 CBB-R 염색시약은 증류수로 제조한 CBB-R 염색시약보다 먼저 단백질을 강하게 염색시켰다. 뿐만 아니라 25℃에서 환원전리수는 일반 물에 비하여 녹차성분에 대해 극히 탁월한 용해력을 나타내었다. 이러한 결과는 환원전리수가 일반 물보다 침투력과 용해력이 매우 강력하다는 것을 보여준다. The permeability of cathodic electrolyzed water toward electrophoretic gel and dissolvability of cathodic electrolyzed water toward green tea components were compared with those of general waters in this investigation. Stained band intensities of the proteins by CBB-R prepared in cathodic electrolyzed water were compared with those in deionized water for various time intervals. Proteins were stained first by CBB-R in cathodic electrolyzed water as compared with those by CBB-R in deionized water. Moreover, cathodic electrolyzed water showed dramatically enhanced solubility toward green tea components at 25℃ than general waters. These results suggest much greater permeability and dissolvability of cathodic electrolyzed water than those of general waters.