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Lyu Su-Yun,Rhim Jee-Young,Park Won-Bong The Pharmaceutical Society of Korea 2005 Archives of Pharmacal Research Vol.28 No.11
Flavonoids, a group of low molecular weight phenylbenzopyrones, have various pharmacological properties including antioxidant, anticancer, bactericidal, and anti-inflammatory. We carried out anti-herpetic assays on 18 flavonoids in five classes and a virus-induced cytopathic effect (CPE) inhibitory assay, plaque reduction assay, and yield reduction assay were performed. When flavonoids were applied at various concentrations to Vero cells infected by HSV-1 and 2, most of the f1avonoids showed inhibitory effects on virus-induced CPE. Among the flavonoids, EC, ECG (flavanols), genistein (isoflavone), naringenin (flavanone), and quercetin (flavonol) showed a high level of CPE inhibitory activity. The antiviral activity of flavonoids were also examined by a plaque reduction assay. EC, ECG, galangin, and kaempferol showed a strong antiviral activity, and catechin, EGC, EGCG, naringenin, chrysin, baicalin, fisetin, myricetin, quercetin, and genistein showed moderate inhibitory effects against HSV-1. In these experiments, flavanols and flavonols appeared to be more active than flavones. Furthermore, treatment of Vero cells with ECG and galangin (which previously showed strong antiviral activities) before virus adsorption led to a slight enhancement of inhibition as determined by a yield reduction assay, indicating that an intracellular effect may also be involved.
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Young Sook Kim(김영숙) 한국생명과학회 2007 생명과학회지 Vol.17 No.10
Nocardiopis sp. 유래의 protein kinase 저해제인 KT5720, KT5926을 사용하여 Vesicular Stomatitis Virus (VSV) 증식에 미치는 세포변성의 억제를 지표로서 연구하였다. 그 결과 cAMP 의존 kinase를 저해하는 KT5720, mysion light chain kinase (MLCK)를 저해하는 KT5926이 VSV 에 의한 세포변성을 억제시켰다. Protein kinase inhibitor KT5720은 VSV의 증식에 영향을 미치지는 않지만, 세포변성과정은 억제하는 것으로 나타났다. Virus RNA 및 단백질 합성에서 PKA KT5720이 영향을 미치지 않는 것은 virus 증식에 영향이 없는 것으로 나타난다. Virus 증식과정에서 성질이 다른 protein kinase가 관여하는데, 이것은 negative strand virus에 대한 항virus 제 개발에 중요한 역할을 한다고 생각한다. I investigated the effect of KT5720, an inhibitor of protein kinase A, on the vesicular stomatitis virus (VSV) infection in BHK- 21cell cultures . The virus inducted cytopathic effect (CPE) was almost completely suppressed by KT5720 at 5uM. The inhibitor, however, did not affect replication of the virus nor the synthesis of viral macromolecules. KT5720, did not block the cytoskeletal disruption, while the cell rounding was suppressed. And, the KT5720-sensitive function may be involved in developing the VSV-induced CPE, but not essential for the virus replications.
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국내분리 porcine reproductive and respiratory syndrome(PRRS) virus의 특성에 관한 연구
장경수,권창희,전무형 충남대학교 수의과대학 동물의과학연구소 1995 動物醫科學硏究誌 Vol.3 No.-
The Korean isolates of porcine reproductive and respiratory syndrom(PRRS) virus, KPRRSV-1 and KPRRSV-2, were characterized on the aspects of sensitivity on physicochemical treatment, typing of nucleic acid, hemagglutinating activity, replication in cultured cells and morphology in electron microscopy. The isolates were labile to ether or chloroform, acid(pH 3.0) or alkali(pH 8.0), and heating(56℃, 30min). The viruses showed no haemagglutinating activity, and inhibition of replication in the medium containing 5-bromo-2'-deoxyuridine. Virus replication was more active in porcine alveolar macrophage(PAM) as compared with that in MARC-145 cell line, showing the maximum titer of 10^4.6TCID_50/㎖ at 5 days post-exposure(pe). Cytopathic effects(CPE) were observed more evidently in the PAM cells than in MARC-145 cell line. In electron microscopy, observation for the isolates showed oval or spherical types of virions, surrounded by a thin membrane-like envelope. The virions were from 45 to 86nm in diameter containing the cores of 35∼50nm in size
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Li, Zubing,Liu, Jing,Zhao, Yifang Korean Society for Biochemistry and Molecular Biol 2005 Journal of biochemistry and molecular biology Vol.38 No.1
GLPG (Ganoderma lucidum proteoglycan) was a bioactive fraction obtained by the liquid fermentation of the mycelia of Ganoderma lucidum, EtOH precipitation, and DEAE-cellulose column chromatography. GLPG was a proteoglycan with a carbohydrate: protein ratio of 10.4: 1. Its antiviral activities against herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) were investigated using a cytopathic inhibition assay. GLPG inhibited cell death in a dose-dependent manner in HSV-infected cells. In addition, it had no cytotoxic effect even at 2 mg/ml. In order to study the mode of action of the antiviral activity of GLPG, cells were treated with GLPG before, during, and after infection, and viral titer in the supernatant of cell culture 48 h post-infection was determined using a $TCID_{50}$ assay. The antiviral effects of GLPG were more remarkable before viral treatment than after treatment. Although the precise mechanism has yet to be defined, our work suggests that GLPG inhibits viral replication by interfering with the early events of viral adsorption and entry into target cells. Thus, this proteoglycan appears to be a candidate anti-HSV agent.
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( Zu Bing Li ),( Jing Liu ),( Yi Fang Zhao ) 생화학분자생물학회 2005 BMB Reports Vol.38 No.1
GLPG (Ganoderma lucidum proteoglycan) was a bioactive fraction obtained by the liquid fermentation of the mycelia of Ganoderma lucidum, EtOH precipitation, and DEAE-cellulose column chromatography. GLPG was a proteoglycan with a carbohydrate: protein ratio of 10.4: 1. Its antiviral activities against herpes simplex virus type 1 (HSV 1) and type 2 (HSV-2) were investigated using a cytopathic inhibition assay. GLPG inhibited cell death in a dose-dependent manner in HSV-infected cells. In addition, it had no cytotoxic effect even at 2 mg/ml. In order to study the mode of action of the antiviral activity of GLPG, cells were treated with GLPG before, during, and after infection, and viral titer in the supernatant of cell culture 48 h post-infection was determined using a TCIDG_(50) assay. The antiviral effects of GLPG were more remarkable before viral treatment than after treatment. Although the precise mechanism has yet to be defined, our work suggests that GLPG inhibits viral replication by interfering with the early events of viral adsorption and entry into target cells. Thus, this proteoglycan appears to be a candidate anti-HSV agent.
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