양이온 폴리머와 음이온/양쪽성 계면활성제로 형성된 코아세르베이트 건조 필름 특성이 샴푸 건조 후 사용감에 미치는 영향

손성길 ( Seong Kil Son ),전현욱 ( Hyun Wook Jeon ),이인호 ( In Ho Lee ),장석윤 ( Sug Youn Chang ) 대한화장품학회 2012 대한화장품학회지 Vol.38 No.2

본 연구는 건조된 코아세르베이트 필름의 물리적 특성이 샴푸 건조 후 모발 사용감에 미치는 상관관계를 조사하기 위하여 실시하였다. 단순 샴푸 조성물은 동일한 조성의 계면활성제에 양이온 전하 밀도가 서로 다른 두 종류의 양이온 폴리머를 사용하여 제조하였다. 이 단순 조성물을 물에 희석하여 코아세르베이트(Coacervate)를 형성되도록 하였고, 3000 rpm, 30 min 조건으로 원심 분리하여 형성된 코아세르베이트를 얻었다. 얻어진 코아세르베이트를 유리판 위에 균일한 두께로 도포하고 50 ℃ 건조기에서 1 h 건조하여 코아세르베이트 필름을 얻었다. 이렇게 얻어진 코아세르베이트 필름의 접촉각과 코아세르베이트의 SEM 이미지 조사를 수행하였고, 코아세르베이트의 수분 보유량과 수분 유지력을 동시에 조사하였다. 샴푸 후 건조된 모발의 부드러움과 보습감은 모발타래를 이용하여 전문 미용 패널이 평가를 수행하였다. 본 실험결과 건조된 코아세르베이트 필름의 특성이 샴푸 후 건조된 모발의 부드러움 및 보습감에 영향을 주는 것으로 확인되었다. The purpose of this study was to examine the correlation between physical properties of dried coacervate films and dry feel for shampoo composition. Simple shampoo compositions were made of two different cationic charge density polymers in the same surfactant compositions. The simple shampoo compositions were diluted with distilled water to make coacervate. Formed coacervate was collected by centrifuge (3,000 rpm, 30 min). Coacervate was coated on the glass plates and dried in drying oven (for 1 h, 50 ℃) to make the thin film. We carried out an experiment on measurement of contact angle, moisture loss ratio and SEM image analysis of the dried coacervate film. Dry feelings of the shampoos were evaluated by panel using hair tresses. Results show that the properties of dried coacervate films affect the dry feel of the after shampooing.

샴푸 코아세르베이트 정량 분석법

송상훈 ( Sang-hun Song ),박봉정 ( Bongjeong Park ),손성길 ( Seong Kil Son ),최수규 ( Soo Gyu Choi ) 대한화장품학회 2018 대한화장품학회지 Vol.44 No.3

본 연구에서는 투과도 평가법, 유리관 정치법과 원심분리 평가법을 통해 샴푸의 코아세르베이트 생성량을 정량화하는 시도를 하였으며, 각 방법의 수행 조건을 달리하여 값의 정확성을 높이고 편차를 적게 하는 가장 최적의 조건을 구해서 평가법의 표준화를 확립하였다. 코아세르베이트가 많이 생성되는 샘플과 적게 생성되는 샘플을 선정하여 서로 비교하여 검증한 결과, 다양한 조건에서의 투과도 값 및 원심분리 값에 대한 재현성과 유의차를 확보하였다. 이를 바탕으로 원심분리법은 빠르고 적은 편차로 코아세르베이트 생성량을 평가할 수 있는 평가법임을 결론내릴 수 있었으며, 건조잔량분과 수분을 포함한 코아세르베이트의 폴리머간 생성 패턴 차이가 없음도 발견하여 액상 상태로 코아세르베이트 함량을 평가할 수 있는 편리한 평가법임을 발견하였다. This study attempts to quantify amount of coacervate generated in a formulation of shampoo with three methods using optical transmission, suspension in a glass tube, and centrifugation. For the correct data acquisition, each method has been optimized to estimate the amount of coacervate with minimum standard deviation. To simply and quantitatively estimate amount of coacervate, two formulations of shampoo were employed as a large or small amount of coacervate generated. Comparisons of the two formulations in repetitive measurement elucidated that the methods by the centrifugation efficiently can be utilized to estimate the accurate amount of coacervate. Additionally, the amount of coacervate by the centrifugation does not exhibit the difference of patterns before and after drying in a shampoo formulation with different conditioning polymers, and this suggests that the centrifugation is a superior method to compare amount of coacervate in liquid.

Iron-clad Complex Coacervate Based Tough Coating of Metalloprotein in Mussel Byssus

Hyungbin KIM,Dong Soo HWANG 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10

The cuticle of byssus, a stiff yet extensible coating, has a biphasic ultra-structure with soft-elastic granules embedded in a hard matrix. The role of the distributed granules in the matrix has been proposed as a stress-damper, however, their physicochemical fundamentals have been poorly explored. Here, we report a toughening process of the cuticle via coacervation and metal-coordination of mussel foot protein-1, a major metalloprotein in the mussel cuticle. We found, when the positively charged self-coacervate of mfp-1 meets positive Fe3+ cation, the positively charged mfp-1 coacervate transforms into the oppositely charged complex coacervate with charge neutralization due to the formation of Fe3+-catechol coordinate complex. Transition from the like-charged coacervate to oppositely charged coacervate due to Fe3+ results in stiff enhancement of the mechanical properties, i.e., 30-fold in viscosity, 16-fold in elasticity, and 20-fold in cohesive energy. And thiol-rich reducing condition provides reversible yet strong sacrificial bonds inside the oppositely charged coacervate. Thus, the neutral mfp-1-Fe3+ complex coacervates, which are granular-like soft-elastomers, induce energy dissipation and self-healing for toughening during tension. And they can switch from the pre-stressed state to repeat stress-damping. The results may provide strategies for the design of tough nature-inspired materials.

Effect of complex coacervate of mussel inspired copolymers

나행요,최재곤,손정선 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.1

Complex coacervation-phase separation occurs between positively and negatively charged in aqueous solutions. It is known that marine mussel has been consist of positively charged, however it can be induced the complex coacervate by strong cation-π interaction. Cation- π interaction was occurred between electron rich π system such as aromatic ring and cations, which it was a non-covalent interaction. In this work, marine mussel inspired, biomimetic water-borne adhesive was synthesized by radical copolymerization with different kinds of acrylate functional group. To better understand the complex coacervate, we investigated the effect of cation-π interaction by various kind of metal cations (Mg²⁺, Ca²⁺, Fe³⁺, V⁵⁺) and catechol of DOPA derivatives with different substrates. We found that catechol chelates metal ions to form strong complex coacervate with metal ions, including Mg²⁺, Ca²⁺, Fe³⁺ and V⁵⁺.

Coacervate-mediated exogenous growth factor delivery for scarless skin regeneration

Park, Uiseon,Lee, Min Suk,Jeon, Jin,Lee, Sangmin,Hwang, Mintai P.,Wang, Yadong,Yang, Hee Seok,Kim, Kyobum Elsevier 2019 Acta Biomaterialia: structure-property-function re Vol.90 No.-

<P><B>Abstract</B></P> <P>Although there are numerous medical applications to recover damaged skin tissue, scarless wound healing is being extensively investigated to provide a better therapeutic outcome. The exogenous delivery of therapeutic growth factors (GFs) is one of the engineering strategies for skin regeneration. This study presents an exogenous GF delivery platform developed using coacervates (Coa), a tertiary complex of poly(ethylene argininyl aspartate diglyceride) (PEAD) polycation, heparin, and cargo GFs (i.e<I>.</I>, transforming growth factor beta 3 (TGF-β3) and interleukin 10 (IL-10)). Coa encompasses the advantage of high biocompatibility, facile preparation, protection of cargo GFs, and sustained GF release. We therefore speculated that coacervate-mediated dual delivery of TGF-β3/IL-10 would exhibit synergistic effects for the reduction of scar formation during physiological wound healing. Our results indicate that the exogenous administration of dual GF via Coa enhances the proliferation and migration of skin-related cells. Gene expression profiles using RT-PCR revealed up-regulation of ECM formation at early stage of wound healing and down-regulation of scar-related genes at later stages. Furthermore, direct injection of the dual GF Coa into the edges of damaged skin in a rat skin wound defect model demonstrated accelerated wound closure and skin regeneration after 3 weeks. Histological evaluation and immunohistochemical staining also revealed enhanced formation of the epidermal layer along with facilitated angiogenesis following dual GF Coa delivery. Based on these results, we conclude that polycation-mediated Coa fabrication and exogenous dual GF delivery via the Coa platform effectively augments both the quantity and quality of regenerated skin tissues without scar formation.</P> <P><B>Statement of Significance</B></P> <P>This study was conducted to develop a simple administration platform for scarless skin regeneration using polycation-based coacervates with dual GFs. Both <I>in vitro</I> and <I>in vivo</I> studies were performed to confirm the therapeutic efficacy of this platform toward scarless wound healing. Our results demonstrate that the platform developed by us enhances the proliferation and migration of skin-related cells. Sequential modulation in various gene expression profiles suggests a balanced collagen-remodeling process by dual GFs. Furthermore, <I>in vivo</I> histological evaluation demonstrates that our technique enhances clear epidermis formation with less scab and thicker woven structure of collagen bundle, similar to that of a normal tissue. We propose that simple administration of dual GFs with Coa has the potential to be applied as a clinical approach for fundamental scarless skin regeneration.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Coacervate microcapsules of vitamin U optimized by central composite design (CCD)

Dae‑Duk Kim,Ji‑Su Kim,Ki‑Taek Kim,Ju‑Hwan Park,Jae‑Young Lee,Min-Hwan Kim,Hyung Geun Min,Ik‑Hwan Moon,Choon‑Young Choi,Bo Hyun Kim 한국약제학회 2019 Journal of Pharmaceutical Investigation Vol.49 No.3

The purpose of this study was to prepare microcapsules encapsulating vitamin U (VU, S-methylmethionine) by complex coacervation. Multiple emulsion method was applied for encapsulating highly water-soluble VU. The composition of microcapsules was optimized using central composite design (CCD). The weight ratio of gelatin to gum arabic (X1) and the volume of oil phase (X2) were set as two independent variables to obtain the maximum weight of microcapsules (Y1) and the content of VU (Y2) in microcapsules higher than 2.5%. When the microcapsules were prepared based on the optimized composition, its actual Y1 and Y2 values were 95% of the predicted values. Morphology of microcapsules observed by the optical and the fluorescence microscopes was round shape. Scanning electron microscopy (SEM) image of internal particles also confirmed that the multiple emulsions were encapsulated in the microcapules. Mean particle size of microcapsules measured by the laser diffraction particle size analyzer was 79.17 μm. Differential scanning calorimetry (DSC) showed that VU exists in an amorphous state in the coacervate microcapsules. Thus, it can be concluded that hydrophilic VU was successfully encapsulated in the coacervate microcapsules using the multiple emulsion method, and the design of experiment (DoE) technique was useful to optimize the formulation.

Gelatin-alginate coacervates for circumventing proteolysis and probing intermolecular interactions by SPR

Lau, Hui-Chong,Jeong, Seonghee,Kim, Aeri Elsevier 2018 INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES Vol.117 No.-

<P><B>Abstract</B></P> <P>Complex coacervates based on natural biopolymers have been explored as a protein delivery system to provide controlled release of loaded protein and circumvent the proteolytic degradation in chronic wounds. The coacervates composed of gelatin A (GA) and sodium alginate (SA) were optimized with respect to turbidity, size, zeta potential, and polydispersity index. Bovine serum albumin (BSA), a model protein, was effectively encapsulated in the coacervates, resulting in protection from trypsin digestion and controlled release. In contrast, EGF was not encapsulated in the same coacervates. Striking difference in the encapsulation efficiencies of BSA and EGF, despite their similar net charges, was attributed to their different levels of binding to GA based on the surface plasmon resonance (SPR) biosensor analysis. In conclusion, GA and SA coacervates can protect the encapsulated protein from proteolytic degradation, demonstrating its potential as a delivery system in the chronic wounds. SPR biosensor is proposed as an analytical tool to study the interactions between polymers and proteins in association with encapsulation efficiency in complex coacervation. The results of EGF studies suggested that GA was not a suitable polymer for EGF encapsulation and therefore, further investigation would be needed to find suitable polymer systems for improved encapsulation efficiency.</P>

Coacervate-mediated novel pancreatic cancer drug Aleuria Aurantia lectin delivery for augmented anticancer therapy

Sungjun Kim,Yunyoung Choi,김교범 한국생체재료학회 2022 생체재료학회지 Vol.26 No.3

Background: Pancreatic cancer, one of the cancers with the highest mortality rate, has very limited clinical treatment. Cancer cells express abnormal glycans on the surface, and some lectins with a high affinity for the glycans induce apoptosis in cancer. In this study, the efficacy of Aleuria Aurantia lectin (AAL) for the treatment of pancreatic cancer was evaluated and the efficacy improvement through AAL delivery with mPEGylated coacervate (mPEG-Coa) was investigated. Methods: AAL was treated with pancreatic cancer cells, PANC-1, and the expression level of caspase-3 and subsequent apoptosis was analyzed. In particular, the anticancer efficacy of AAL was compared with that of concanavalin A, one of the representative anticancer lectins. Then, methoxypolyethylene glycol-poly(ethylene arginylaspartate diglyceride), a polycation, was synthesized, and an mPEG-Coa complex was prepared with polyanion heparin. The AAL was incorporated into the mPEG-Coa and the release kinetics of the AAL from the mPEG-Coa and the cargo protection capacity of the mPEG-Coa were evaluated. Finally, improved anticancer ability through Coamediated AAL delivery was assessed. Results: These results indicated that AAL is a potential effective pancreatic cancer treatment. Moreover, mPEGCoa rapidly released AAL at pH 6.5, an acidic condition in the cancer microenvironment. The initial rapid release of AAL effectively suppressed pancreatic cancer cells, and the continuous supply of AAL through the Coa transporter effectively inhibited proliferation recurrence of cancer cells. Conclusion: AAL is a potential novel drug for the treatment of pancreatic cancer therapeutic agent. In addition, a continuous supply of drugs above the therapeutic threshold using mPEG-Coa could improve therapeutic efficacy.

Controllable and Localized Co-Delivery of TRAIL and 6-MP Using Coacervate-Embedded Composite Hydrogels for Enhanced Anticancer Therapy

좌예림,김교범 한국공업화학회 2021 한국공업화학회 연구논문 초록집 Vol.2021 No.-

Coacervate-mediated tumor cell lysate delivery for immune cell activation and cancer vaccination

성지현,김교범 한국공업화학회 2021 한국공업화학회 연구논문 초록집 Vol.2021 No.0