흰쥐에서 신장 부분 절제 후 Oxidized Regenerated Cellulose 및 돼지 Small Intestine Submucosa를 삽입 후 생체 적합성 비교

한웅규,김세훈,박종필,양승철 대한비뇨의학회 2008 Investigative and Clinical Urology Vol.49 No.1

Purpose: The objective of this study was to evaluate the biological properties of a variety of materials that could be used in partial nephrectomy. Materials and Methods: 54 Sprague-Dawley rats(9 for each group) divided into an experimental period of 4 and 12 weeks, respectively. 2 other groups were the negative control for this experiment. We inserted oxidized regenerated cellulose and porcine small intestine submucosa into the defected renal parenchyme in the experimental groups. At the end of the observation periods, the animals were killed and their specimens prepared for histological examination to evaluate the different materials biocompatibility. Results: The reaction of the tissue to the materials diminished with time. After analyzing both periods, the inflammatory reactions to oxidized regenerated cellulose and porcine small intestine submucosa were considered slight. There were no significant differences between oxidized regenerated cellulose and porcine small intestine submucosa. Oxidized regenerated cellulose and porcine small intestine submucosa showed biocompatibility in this test model at both time periods. Conclusions: It was concluded that there was no difference of biocompatibility between oxidized regenerated cellulose and porcine small intestine submucosa. Both materials are safe, biocompatible materials that can be inserted into the renal parenchyma. (Korean J Urol 2008;49:43-48) Purpose: The objective of this study was to evaluate the biological properties of a variety of materials that could be used in partial nephrectomy. Materials and Methods: 54 Sprague-Dawley rats(9 for each group) divided into an experimental period of 4 and 12 weeks, respectively. 2 other groups were the negative control for this experiment. We inserted oxidized regenerated cellulose and porcine small intestine submucosa into the defected renal parenchyme in the experimental groups. At the end of the observation periods, the animals were killed and their specimens prepared for histological examination to evaluate the different materials biocompatibility. Results: The reaction of the tissue to the materials diminished with time. After analyzing both periods, the inflammatory reactions to oxidized regenerated cellulose and porcine small intestine submucosa were considered slight. There were no significant differences between oxidized regenerated cellulose and porcine small intestine submucosa. Oxidized regenerated cellulose and porcine small intestine submucosa showed biocompatibility in this test model at both time periods. Conclusions: It was concluded that there was no difference of biocompatibility between oxidized regenerated cellulose and porcine small intestine submucosa. Both materials are safe, biocompatible materials that can be inserted into the renal parenchyma. (Korean J Urol 2008;49:43-48)

A Comparative Biocompatibility Study of Chondrocyte-derived ECM and Silk Fibroin Scaffolds In Vitro and in Rat Acute Traumatic Brain Injury

( Kil Hwan Kim ),( Moon Hang Kim ),( Yoon Hee Lim ),( So Ra Park ),( Byung Hyune Choi ),( Hyung Chun Park ),( Seung Hwan Yoon ),( Byoung Hyun Min ),( Hyeon Seon Park ) 한국조직공학·재생의학회 2009 조직공학과 재생의학 Vol.6 No.14

Traumatic brain injury (TBI) often leads to critical tissue damage and loss. Brain tissue engineering is a promising technique for cellular replacement and neurological functional recovery in animals. However, useful biomaterial scaffolds have not been found so far. In order to explore the feasibility of stem cell based brain tissue engineering, it is first necessary to evaluate the biocompatibility of biomaterials with stem cells and brain tissues. In the present study, two biomaterials, chondrocyte derived ECM (ECM) and silk fibroin (SF) scaffolds, were investigated for their biocompatibility in vitro and in acute TBI. We found that when the ECM scaffold was cultured with rat mesenchymal stem cells (rMSCs) or implanted directly into brain injury, had a good biocompatibility. rMSCs, seeded on the ECM scaffold, were evenly distributed with a spindle shape and attached well, while on SF scaffold attached with a round shape relatively weakly. In the TBI model, the SF scaffold resulted in a significant increase in the cavity volume at 6 weeks postimplantation, but not the ECM scaffold. In addition, the ECM scaffold induced a lower inflammatory response, with active microglia/macrophages at 1 week, than the SF scaffold and control. Interestingly, both scaffolds reduced glial fibrillary acidic protein (GFAP) and neurocan expression in the cavity boundary throughout the experimental periods. These results indicate that the scaffolds tested inhibit the glial scar formation in injured brain tissue. Moreover, there were no significant differences in the neurological outcome and weight body gain, when compared with the brain injury alone. Taken together, these findings reveal that the ECM scaffold showed good biocompatibility to the donor rMSCs and host brain tissue. Thus, the ECM scaffold seems to be beneficial as a biomaterial for brain tissue engineering.

The biocompatibility and mechanical properties of plasma sprayed zirconia coated abutment

Huang, Zhengfei,Wang, Zhifeng,Yin, Kaifeng,Li, Chuanhua,Guo, Meihua,Lan, Jing The Korean Academy of Prosthodonitics 2020 The Journal of Advanced Prosthodontics Vol.12 No.3

PURPOSE. The aim of this study was to evaluate the clinical performance and reliability of plasma sprayed nanostructured zirconia (NSZ) coating. MATERIALS AND METHODS. This study consisted of three areas of analysis: (1) Mechanical property: surface roughness of NSZ coating and bond strength between NSZ coating and titanium specimens were measured, and the microstructure of bonding interface was also observed by scanning election microscope (SEM). (2) Biocompatibility: hemolysis tests, cell proliferation tests, and rat subcutaneous implant test were conducted to evaluate the biocompatibility of NSZ coating. (3) Mechanical compatibility: fracture and artificial aging tests were performed to measure the mechanical compatibility of NSZ-coated titanium abutments. RESULTS. In the mechanical study, 400 ㎛ thick NSZ coatings had the highest bond strength (71.22 ± 1.02 MPa), and a compact transition layer could be observed. In addition, NSZ coating showed excellent biocompatibility in both hemolysis tests and cell proliferation tests. In subcutaneous implant test, NSZ-coated plates showed similar inflammation elimination and fibrous tissue formation processes with that of titanium specimens. Regarding fatigue tests, all NSZ-coated abutments survived in the five-year fatigue test and showed sufficient fracture strength (407.65-663.7 N) for incisor teeth. CONCLUSION. In this study, the plasmasprayed NSZ-coated titanium abutments presented sufficient fracture strength and biocompatibility, and it was demonstrated that plasma spray was a reliable method to prepare high-quality zirconia coating.

연속 다공질 생체세라믹에 대한 생체친화성 연구

송호연,고광균 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

Continuously Al_(2)O_(3) porous bodies were fabricated by multi-extrusion process. Their biocompatibility was evaluated through in vitro and in-vivo study. Behavior of osteoblast adhesion and proliferation grown on Al_(2)O_(3) porous body was observed. Human osteoblast-like MG-63 cells were grown well on top, bottom, and outside as well as inside surface of porous body. Al_(2)O_(3) the ideal pore size was 150-200 um. The 3- dimensional continuously porous Al_(2)O_(3) body was implanted in subcutaneous tissue of rat for in-vivo study. The porous body was covered with well-developed fibrous tissues and showed the formation of new capillaries in it. No inflammatory response around porous body was found. From the results, the bio-ceramic materials used in the research was proved to have excellent biocompatibility.

Biocompatible PEG Grafting on DLC-coated Nitinol Alloy for Vascular Stents

Shin, Hong-Sub,Park, Kwideok,Ji Heung Kim,,Kim, Jae-Jin,Dong Keun Han,,Moon, Myoung-Woon,Lee, Kwang-Ryeol,Ji Hoon Shin, SAGE Publications 2009 Journal of bioactive and compatible polymers Vol.24 No.4

<P>The surfaces of Nitinol (TiNi), a popular metal alloy for arterial stents were thin-coated with diamond-like carbon (DLC) and then grafted with poly(ethylene glycol) (PEG) to increase biocompatibility. The TiNi control, DLC-coated TiNi (TiNi—DLC), and the PEG-grafted TiNi—DLC (TiNi—DLC—PEG) surface characteristics and biocompatibility were evaluated. The hydrophilicity of the TiNi—DLC—PEG significantly increased and the amount of both oxygen and nitrogen on the TiNi—DLC—PEG also increased compared to the TiNi control and TiNi—DLC due to the grafted PEG. The ratio between albumin and fibrinogen was higher on the PEG-grafted surface than the other surfaces when tested with human blood components; the platelet adhesion decreased the most on the TiNi—DLC—PEG surface, indicating improved blood compatibility. For in vivo tests using a rat model, the samples that were implanted for 6 weeks formed fibrous tissue; the tissue layer was much thinner on the PEG-grafted sample than the other two groups. The present results indicate that PEG-grafted TiNi—DLC surface may be effective in enhancing biocompatibility of blood-contacting biomaterials including vascular stents.</P>

Biocompatibility evaluation of peo-treated magnesium alloy implants placed in rabbit femur condyle notches and paravertebral muscles

Seong Ryoung Kim,Keon-Mo Lee,Jin Hong Kim,Young Jin Choi,Han Ick Park,Hwa Chul Jung,Hyung Jin Roh,Jee Hye Lo Han,Joon Rae Kim,이부규 한국생체재료학회 2022 생체재료학회지 Vol.26 No.3

Background: Magnesium alloys have been receiving much attention for use in biodegradable metal implants because of their excellent mechanical properties and biocompatibility. However, their rapid breakdown and low bioactivity can cause the implant to lose mechanical integrity before the bone is completely healed. Moreover, hydrogen gas released during degradation can significantly delay the tissue regeneration process. To solve the instability of magnesium alloys, Zn and Ca can be added to improve the mechanical properties and biocompatibility. One other way to improve the mechanical properties of Mg is plasma electrolytic oxidation (PEO), which provides a dense, thick ceramic-like coating on the Mg surface. In this study, high-purity Mg was selected as the control, and Mg-1wt%Zn0.1wt%Ca alloy and PEO-treated Mg-1wt%Zn-0.1wt%Ca alloy were selected as the test materials; the results of radiographic and histological analyses of their biocompatibility are reported herein. Materials and method: Nineteen New Zealand white rabbits were used in the study. Rod-bars (Ø2.7 × 13.6 mm) were placed on both paravertebral muscles, and cannulated screws (Ø2.7x10mm) were placed on both femur condyle notches. Each animal was implanted in all four sites. X-rays were taken at 0, 2, 4, 8, and 12 weeks, micro-CT, and live-CT were taken at 4, 8, and 12 weeks. At weeks 4, 8, and 12, individuals representing each group were selected and sacrificed to prepare specimens for histopathological examination. Result: The results confirm that in vivo, Mg-1wt%Zn-0.1wt%Ca alloy had higher corrosion resistance than high-purity Mg and safely degraded over time without causing possible side effects (foreign body or inflammatory reactions, etc.). In addition, PEO treatment of Mg-1wt%Zn-0.1wt%Ca alloy had a positive effect on fracture recovery by increasing the bonding area with bone. Conclusion: Our results suggest that PEO treatment of Mg-1wt%Zn-0.1wt%Ca alloy can be a promising biomaterials in the field of various clinical situations such as orthopedic and maxillofacial surgerys.

A green chemistry approach for synthesizing biocompatible gold nanoparticles

Gurunathan, Sangiliyandi,Han, JaeWoong,Park, Jung Hyun,Kim, Jin-Hoi Springer 2014 NANOSCALE RESEARCH LETTERS Vol.9 No.1

<P>Gold nanoparticles (AuNPs) are a fascinating class of nanomaterial that can be used for a wide range of biomedical applications, including bio-imaging, lateral flow assays, environmental detection and purification, data storage, drug delivery, biomarkers, catalysis, chemical sensors, and DNA detection. Biological synthesis of nanoparticles appears to be simple, cost-effective, non-toxic, and easy to use for controlling size, shape, and stability, which is unlike the chemically synthesized nanoparticles. The aim of this study was to synthesize homogeneous AuNPs using pharmaceutically important <I>Ganoderma</I> spp<I>.</I> We developed a simple, non-toxic, and green method for water-soluble AuNP synthesis by treating gold (III) chloride trihydrate (HAuCl<SUB>4</SUB>) with a hot aqueous extract of the <I>Ganoderma</I> spp<I>.</I> mycelia. The formation of biologically synthesized AuNPs (bio-AuNPs) was characterized by ultraviolet (UV)-visible absorption spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), energy dispersive X-ray (EDX), dynamic light scattering (DLS), and transmission electron microscopy (TEM). Furthermore, the biocompatibility of as-prepared AuNPs was evaluated using a series of assays, such as cell viability, lactate dehydrogenase leakage, and reactive oxygen species generation (ROS) in human breast cancer cells (MDA-MB-231). The color change of the solution from yellow to reddish pink and strong surface plasmon resonance were observed at 520 nm using UV-visible spectroscopy, and that indicated the formation of AuNPs. DLS analysis revealed the size distribution of AuNPs in liquid solution, and the average size of AuNPs was 20 nm. The size and morphology of AuNPs were investigated using TEM. The biocompatibility effect of as-prepared AuNPs was investigated in MDA-MB-231 breast cancer cells by using various concentrations of AuNPs (10 to 100 μM) for 24 h. Our findings suggest that AuNPs are non-cytotoxic and biocompatible. To the best of our knowledge, this is the first report to describe the synthesis of monodispersed, biocompatible, and soluble AuNPs with an average size of 20 nm using <I>Ganoderma</I> spp. This study opens up new possibilities of using an inexpensive and non-toxic mushroom extract as a reducing and stabilizing agent for the synthesis of size-controlled, large-scale, biocompatible, and monodispersed AuNPs, which may have future diagnostic and therapeutic applications.</P>

Development, characterisation and biocompatibility testing of a cobalt-containing titanium phosphate-based glass for engineering of vascularized hard tissues

Lee, I.H.,Yu, H.s.,Lakhkar, N.J.,Kim, H.W.,Gong, M.S.,Knowles, J.C.,Wall, I.B. Elsevier 2013 Materials science & engineering. C, Materials for Vol.33 No.4

There is a continuing need to develop scaffold materials that can promote vascularisation throughout the tissue engineered construct. This study investigated the effect of cobalt oxide (CoO) doped into titanium phosphate glasses on material properties, biocompatibility and vascular endothelial growth factor (VEGF) secretion by osteoblastic MG63 cells. Glasses composed of (P<SUB>2</SUB>O<SUB>5</SUB>)<SUB>45</SUB>(Na<SUB>2</SUB>O)<SUB>20</SUB>(TiO<SUB>2</SUB>)<SUB>05</SUB>(CaO)<SUB>30-x</SUB>(CoO)<SUB>x</SUB>(x=0, 5, 10, and 15mol%) were fabricated and the effect of Co on physicochemical properties including density, glass transition temperature (T<SUB>g</SUB>), degradation rate, ion release, and pH changes was assessed. The results showed that incorporation of CoO into the glass system produced an increase in density with little change in T<SUB>g</SUB>. It was then confirmed that the pH did not change significantly when CoO was incorporated in the glass, and stayed constant at around 6.5-7.0 throughout the dissolution study period of 336h. Ion release results followed a specific pattern with increasing amounts of CoO. In general, although incorporation of CoO into a titanium phosphate glass increased its density, other bulk and surface properties of the glass did not show any significant changes. Cell culture studies performed using MG63 cells over a 7-day period indicated that the glasses provide a stable surface for cell attachment and are biocompatible. Furthermore, VEGF secretion was significantly enhanced on all glasses compared with standard tissue culture plastic and Co doping enhanced this effect further. In conclusion, the developed Co-doped glasses are stable and biocompatible and thus offer enhanced potential for engineering vascularized tissue.

Synthesis and Biocompatibility Characterizations of in Situ Chondroitin Sulfate–Gelatin Hydrogel for Tissue Engineering

방수미,정의원,노인섭 한국조직공학과 재생의학회 2018 조직공학과 재생의학 Vol.15 No.1

Novel hydrogel composed of both chondroitin sulfate (CS) and gelatin was developed for better cellular interaction through two step double crosslinking of N-(3-diethylpropyl)-N-ethylcarbodiimide hydrochloride (EDC) chemistries and then click chemistry. EDC chemistry was proceeded during grafting of amino acid dihydrazide (ADH) to carboxylic groups in CS and gelatin network in separate reactions, thus obtaining CS–ADH and gelatin–ADH, respectively. CS–acrylate and gelatin–TCEP was obtained through a second EDC chemistry of the unreacted free amines of CS–ADH and gelatin–ADH with acrylic acid and tri(carboxyethyl)phosphine (TCEP), respectively. In situ CS–gelatin hydrogel was obtained via click chemistry by simple mixing of aqueous solutions of both CS–acrylate and gelatin–TCEP. ATR-FTIR spectroscopy showed formation of the new chemical bonds between CS and gelatin in CS–gelatin hydrogel network. SEM demonstrated microporous structure of the hydrogel. Within serial precursor concentrations of the CS–gelatin hydrogels studied, they showed trends of the reaction rates of gelation, where the higher concentration, the quicker the gelation occurred. In vitro studies, including assessment of cell viability (live and dead assay), cytotoxicity, biocompatibility via direct contacts of the hydrogels with cells, as well as measurement of inflammatory responses, showed their excellent biocompatibility. Eventually, the test results verified a promising potency for further application of CS–gelatin hydrogel in many biomedical fields, including drug delivery and tissue engineering by mimicking extracellular matrix components of tissues such as collagen and CS in cartilage.

Performances and Biocompatibility of Prepared pH-Sensitive Cotton/Polyamide 6 Medical Dressings Dyed with Halochromic Dyes

Roberta Peila,Alessio Varesano,Claudia Vineis,Roberta Cavalli,Monica Argenziano,Barbara Cravello,Cinzia Tonetti 한국섬유공학회 2022 Fibers and polymers Vol.23 No.8

A natural pH-sensitive dye, curcumin, and a synthetic pH-sensitive dye, Bromothymol blue, were successfullyapplied on cotton/polyamide 6 medical dressings. The work addresses two different topics: on the one hand the dyeingprocess of the medical devices; on the other hand, the evaluation of the dyed medical dressing biocompatibility. Differentdye concentrations were tested to find the best dye exhaustion and dye fastness to washing and perspiration. The halochromictest evidenced a pH sensitivity in a range of pH from 5.5 to 8.0 and 8.5. These properties confirmed their possible usage aspotential pH textile sensors in the control of skin diseases. The last sections examine the biocompatibility of the preparedmedical devices with human skin. MTT assay on in vitro cultures of human epithelial cells (HaCaT keratinocytes) wasperformed to test the cytotoxicity. In vitro permeation studies with Franz cells were performed as well to evaluate the dyepotential accumulation in the skin layers. No cytotoxicity was detected as well as no dye permeation through the skin layers.